首页 > 最新文献

Bioengineering & Translational Medicine最新文献

英文 中文
Correction to “Self-assembly of PEG–PPS polymers and LL-37 peptide nanomicelles improves the oxidative microenvironment and promotes angiogenesis to facilitate chronic wound healing” 更正 "PEG-PPS 聚合物和 LL-37 肽纳米微孔的自组装可改善氧化微环境并促进血管生成,从而促进慢性伤口愈合"
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-09 DOI: 10.1002/btm2.10718

Shi R, Qiao J, Sun Q, Hou B, Li B, Zheng J, Zhang Z, Peng Z, Zhou J, Shen B, Deng J, Zhang X. Self-assembly of PEG–PPS polymers and LL-37 peptide nanomicelles improves the oxidative microenvironment and promotes angiogenesis to facilitate chronic wound healing. Bioeng Transl Med. 2023;9(2):e10619. doi:10.1002/btm2.10619

The authors regret some errors have been found in Figure 5, Figure S12, and Figure S15.

In Figure 5, due to the misuse of wound images of the LL-37@PEG–PPS group on day 9, there was a duplication with the wound images of the PEG–PPS group on day 11.

In Figure S12a, due to misuse of images, there was partial overlap of the 0 h images between the control group and PEG–PPS group.

In Figure S15a, unintentional misuse of the in vivo biodistribution image of FITC-LL-37@PEG–PPS in before injection group, which leads to an overlapped with that on day 4.

Shi R、Qiao J、Sun Q、Hou B、Li B、Zheng J、Zhang Z、Peng Z、Zhou J、Shen B、Deng J、Zhang X. PEG-PPS 聚合物和 LL-37 肽纳米小室的自组装改善了氧化微环境并促进了血管生成,从而促进了慢性伤口愈合。Bioeng Transl Med.2023;9(2):e10619. doi:10.1002/btm2.10619作者对图 5、图 S12 和图 S15 中发现的一些错误表示遗憾。在图 5 中,由于误用了第 9 天 LL-37@PEG-PPS 组的伤口图像,导致与第 11 天 PEG-PPS 组的伤口图像重复。图 S12a 中,由于误用图像,对照组和 PEG-PPS 组的 0 h 图像有部分重叠。图 S15a 中,注射前组的 FITC-LL-37@PEG-PPS 体内生物分布图像被无意误用,导致与第 4 天的图像重叠。
{"title":"Correction to “Self-assembly of PEG–PPS polymers and LL-37 peptide nanomicelles improves the oxidative microenvironment and promotes angiogenesis to facilitate chronic wound healing”","authors":"","doi":"10.1002/btm2.10718","DOIUrl":"10.1002/btm2.10718","url":null,"abstract":"<p>Shi R, Qiao J, Sun Q, Hou B, Li B, Zheng J, Zhang Z, Peng Z, Zhou J, Shen B, Deng J, Zhang X. Self-assembly of PEG–PPS polymers and LL-37 peptide nanomicelles improves the oxidative microenvironment and promotes angiogenesis to facilitate chronic wound healing. <i>Bioeng Transl Med</i>. 2023;9(2):e10619. doi:10.1002/btm2.10619</p><p>The authors regret some errors have been found in Figure 5, Figure S12, and Figure S15.</p><p>In Figure 5, due to the misuse of wound images of the LL-37@PEG–PPS group on day 9, there was a duplication with the wound images of the PEG–PPS group on day 11.</p><p>In Figure S12a, due to misuse of images, there was partial overlap of the 0 h images between the control group and PEG–PPS group.</p><p>In Figure S15a, unintentional misuse of the in vivo biodistribution image of FITC-LL-37@PEG–PPS in before injection group, which leads to an overlapped with that on day 4.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Doxorubicin-loaded nanoparticle coated with endothelial cells-derived exosomes for immunogenic chemotherapy of glioblastoma” 对 "涂有内皮细胞衍生外泌体的多柔比星负载纳米粒子用于胶质母细胞瘤的免疫原性化疗 "的更正
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-05 DOI: 10.1002/btm2.10719

Zhang C, Song J, Lou L, et al. Doxorubicin-loaded nanoparticle coated with endothelial cells-derived exosomes for immunogenic chemotherapy of glioblastoma. Bioeng Transl Med 2020;6(3):e10203.

We apologize for this error.

Zhang C, Song J, Lou L, et al. 内皮细胞衍生的外泌体包被的多柔比星纳米颗粒用于胶质母细胞瘤的免疫原性化疗。Bioeng Transl Med 2020;6(3):e10203.We apologize for this error.
{"title":"Correction to “Doxorubicin-loaded nanoparticle coated with endothelial cells-derived exosomes for immunogenic chemotherapy of glioblastoma”","authors":"","doi":"10.1002/btm2.10719","DOIUrl":"10.1002/btm2.10719","url":null,"abstract":"<p>Zhang C, Song J, Lou L, et al. Doxorubicin-loaded nanoparticle coated with endothelial cells-derived exosomes for immunogenic chemotherapy of glioblastoma. Bioeng Transl Med 2020;6(3):e10203.</p><p>We apologize for this error.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In situ-crosslinked Zippersomes enhance cardiac repair by increasing accumulation and retention 原位交联的 Zippersomes 可通过增加积累和保留来促进心脏修复。
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-08-20 DOI: 10.1002/btm2.10697
Natalie E. Jasiewicz, Kuo-Ching Mei, Hannah M. Oh, Emily E. Bonacquisti, Ameya Chaudhari, Camryn Byrum, Brian C. Jensen, Juliane Nguyen

Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are a promising treatment for myocardial infarction (MI), but their therapeutic efficacy is limited by inefficient accumulation at the target site. A minimally invasive MSC EV therapy that enhances EV accumulation at the disease site and extends EV retention could significantly improve post-infarct cardiac regeneration. Here, we show that EVs decorated with the next-generation of high-affinity (HiA) heterodimerizing leucine zippers, termed HiA Zippersomes, amplify targetable surface areas through in situ crosslinking and exhibited ~7-fold enhanced accumulation within the infarcted myocardium in mice after 3 days and continued to be retained up to Day 21, surpassing the performance of unmodified EVs. After MI in mice, HiA Zippersomes increase the ejection fraction by 53% and 100% compared with unmodified EVs and phosphate-buffered saline (PBS), respectively. This notable improvement in cardiac function played a crucial role in restoring healthy heart performance. HiA Zippersomes also robustly decrease infarct size by 52% and 60% compared with unmodified EVs and PBS, respectively, thus representing a promising platform for minimally invasive vesicle delivery to the infarcted heart compared to intramyocardial injections.

间充质干细胞(MSC)衍生的细胞外囊泡(EVs)是治疗心肌梗死(MI)的一种有前景的方法,但其疗效因在目标部位的低效积累而受到限制。微创间充质干细胞EV疗法能增强EV在疾病部位的积累并延长EV的保留时间,从而显著改善梗死后的心脏再生。在这里,我们展示了用新一代高亲和力(HiA)异二聚体亮氨酸拉链(称为 HiA Zippersomes)装饰的 EVs,它们通过原位交联扩大了可靶向的表面区域,3 天后在小鼠梗死心肌内的蓄积增强了约 7 倍,并持续保留到第 21 天,超过了未修饰 EVs 的表现。小鼠发生心肌梗死后,与未修饰的 EVs 和磷酸盐缓冲盐水(PBS)相比,HiA Zippersomes 可使射血分数分别提高 53% 和 100%。心脏功能的显著改善在恢复健康心脏性能方面发挥了关键作用。与未修饰的EVs和磷酸盐缓冲盐水(PBS)相比,HiA Zippersomes还能使梗死面积分别缩小52%和60%,因此与心肌内注射相比,HiA Zippersomes是向梗死心脏提供微创囊泡的理想平台。
{"title":"In situ-crosslinked Zippersomes enhance cardiac repair by increasing accumulation and retention","authors":"Natalie E. Jasiewicz,&nbsp;Kuo-Ching Mei,&nbsp;Hannah M. Oh,&nbsp;Emily E. Bonacquisti,&nbsp;Ameya Chaudhari,&nbsp;Camryn Byrum,&nbsp;Brian C. Jensen,&nbsp;Juliane Nguyen","doi":"10.1002/btm2.10697","DOIUrl":"10.1002/btm2.10697","url":null,"abstract":"<p>Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are a promising treatment for myocardial infarction (MI), but their therapeutic efficacy is limited by inefficient accumulation at the target site. A minimally invasive MSC EV therapy that enhances EV accumulation at the disease site and extends EV retention could significantly improve post-infarct cardiac regeneration. Here, we show that EVs decorated with the next-generation of high-affinity (HiA) heterodimerizing leucine zippers, termed HiA Zippersomes, amplify targetable surface areas through in situ crosslinking and exhibited ~7-fold enhanced accumulation within the infarcted myocardium in mice after 3 days and continued to be retained up to Day 21, surpassing the performance of unmodified EVs. After MI in mice, HiA Zippersomes increase the ejection fraction by 53% and 100% compared with unmodified EVs and phosphate-buffered saline (PBS), respectively. This notable improvement in cardiac function played a crucial role in restoring healthy heart performance. HiA Zippersomes also robustly decrease infarct size by 52% and 60% compared with unmodified EVs and PBS, respectively, thus representing a promising platform for minimally invasive vesicle delivery to the infarcted heart compared to intramyocardial injections.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11558206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Activation of NR1H3 attenuates the severity of septic myocardial injury by inhibiting NLRP3 inflammasome” 对 "通过抑制 NLRP3 炎症小体激活 NR1H3 减轻脓毒症心肌损伤的严重程度 "的更正
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-08-15 DOI: 10.1002/btm2.10707

Chao Deng, Qiong Liu, Huadong Zhao, Lu Qian, Wagnrui Lei, Wenwen Yang, Zhenxing Liang, Ye Tian, Shaofei Zhang, Changyu Wang, Ying Chen, Yang Yang. Activation of NR1H3 attenuates the severity of septic myocardial injury by inhibiting NLRP3 inflammasome. Bioeng Transl Med. 2023; 8(3):e10517.

An inaccuracy has been found in the statistical graph of ABCA1 in Figure 2c of the published article. The corrected version of Figure 2 is shown below.

We apologize for this error.

邓超、刘琼、赵华东、钱璐、雷万瑞、杨雯雯、梁振兴、田野、张少飞、王长宇、陈颖、杨洋 通过抑制NLRP3炎性体激活NR1H3减轻脓毒症心肌损伤的严重程度Bioeng Transl Med. 2023;8(3):e10517.在已发表文章的图 2c 中发现 ABCA1 的统计图表有误。我们对这一错误表示歉意。
{"title":"Correction to “Activation of NR1H3 attenuates the severity of septic myocardial injury by inhibiting NLRP3 inflammasome”","authors":"","doi":"10.1002/btm2.10707","DOIUrl":"10.1002/btm2.10707","url":null,"abstract":"<p>\u0000 <span>Chao Deng</span>, <span>Qiong Liu</span>, <span>Huadong Zhao, Lu</span> <span>Qian, Wagnrui Lei</span>, <span>Wenwen Yang</span>, <span>Zhenxing Liang</span>, <span>Ye Tian</span>, <span>Shaofei Zhang</span>, <span>Changyu Wang</span>, <span>Ying Chen</span>, <span>Yang Yang</span>. <span>Activation of NR1H3 attenuates the severity of septic myocardial injury by inhibiting NLRP3 inflammasome</span>. <i>Bioeng Transl Med</i>. <span>2023</span>; <span>8</span>(<span>3</span>):e10517.</p><p>An inaccuracy has been found in the statistical graph of ABCA1 in Figure 2c of the published article. The corrected version of Figure 2 is shown below.</p><p>We apologize for this error.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141986248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sperm quality analyzer: A portable LED array microscope with dark-field imaging 精子质量分析仪具有暗视野成像功能的便携式 LED 阵列显微镜
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-08-02 DOI: 10.1002/btm2.10703
Meng Shao, Changxu Li, Xiaohao Ma, Haoyu Pan, Zeyu Ke, Rui Liu, Zhiguo Zhang, Min-Cheng Zhong, Yi Wang, Zhensheng Zhong, Fengya Lu, Xunbin Wei, Jinhua Zhou

Sperm quality analysis plays an important role in diagnosing infertility, which is widely implemented by computer-assisted sperm analysis (CASA) of sperm-swimming imaging from commercial phase-contrast microscopy. A well-equipped microscope comes with a high cost, increasing the burden of assessment, and it also occupies a large volume. For point-of-care testing (POCT) of sperm quality, these factors are confronted with the challenges of low-cost and portable instruments. In this study, an encoded light-emitting diode (LED) array illumination is employed to achieve a portable microscope with multicontrast imaging for sperm quality analysis. This microscopy has dimensions of 16.5 × 14.0 × 25.0 cm, and its dark-field (DF) imaging provides high-contrast sperm image data which is suitable for CASA. According to DF imaging, we developed a software of LabCASA, which can used to assess the motility characteristics of sperm. Compared with TrackMate, the difference in motility parameters from our software was less than 10% in the coefficient of variation (CV). The sperm motility parameters vary with the chamber temperature, which further confirms the reliability of our system with DF imaging. The DF imaging provides strong robustness for tracking sperm's motion under different microscopes. For assessment of the motility parameters, our system can work at a lower cost with a plastic structure. This system with DF imaging is suitable for portable POCT of sperm quality analysis, which is highly cost-effective in resource-constrained circumstances.

精子质量分析在不孕症诊断中起着重要作用,目前广泛采用的是商用相差显微镜的精子游动成像计算机辅助精子分析(CASA)。设备齐全的显微镜价格昂贵,增加了评估负担,而且体积庞大。对于精子质量的护理点检测(POCT)来说,这些因素都面临着低成本和便携式仪器的挑战。在这项研究中,采用了编码发光二极管(LED)阵列照明技术,实现了用于精子质量分析的便携式多对比度成像显微镜。该显微镜的尺寸为 16.5 × 14.0 × 25.0 厘米,其暗视野(DF)成像可提供适合 CASA 的高对比度精子图像数据。根据暗视野成像技术,我们开发了 LabCASA 软件,用于评估精子的运动特性。与 TrackMate 相比,我们软件的精子活力参数变异系数(CV)相差不到 10%。精子运动参数随腔室温度的变化而变化,这进一步证实了我们的 DF 成像系统的可靠性。DF 成像技术为在不同显微镜下跟踪精子运动提供了强大的稳健性。在运动参数评估方面,我们的系统采用塑料结构,成本较低。这种带 DF 成像的系统适用于精子质量分析的便携式 POCT,在资源有限的情况下极具成本效益。
{"title":"Sperm quality analyzer: A portable LED array microscope with dark-field imaging","authors":"Meng Shao,&nbsp;Changxu Li,&nbsp;Xiaohao Ma,&nbsp;Haoyu Pan,&nbsp;Zeyu Ke,&nbsp;Rui Liu,&nbsp;Zhiguo Zhang,&nbsp;Min-Cheng Zhong,&nbsp;Yi Wang,&nbsp;Zhensheng Zhong,&nbsp;Fengya Lu,&nbsp;Xunbin Wei,&nbsp;Jinhua Zhou","doi":"10.1002/btm2.10703","DOIUrl":"10.1002/btm2.10703","url":null,"abstract":"<p>Sperm quality analysis plays an important role in diagnosing infertility, which is widely implemented by computer-assisted sperm analysis (CASA) of sperm-swimming imaging from commercial phase-contrast microscopy. A well-equipped microscope comes with a high cost, increasing the burden of assessment, and it also occupies a large volume. For point-of-care testing (POCT) of sperm quality, these factors are confronted with the challenges of low-cost and portable instruments. In this study, an encoded light-emitting diode (LED) array illumination is employed to achieve a portable microscope with multicontrast imaging for sperm quality analysis. This microscopy has dimensions of 16.5 × 14.0 × 25.0 cm, and its dark-field (DF) imaging provides high-contrast sperm image data which is suitable for CASA. According to DF imaging, we developed a software of LabCASA, which can used to assess the motility characteristics of sperm. Compared with TrackMate, the difference in motility parameters from our software was less than 10% in the coefficient of variation (CV). The sperm motility parameters vary with the chamber temperature, which further confirms the reliability of our system with DF imaging. The DF imaging provides strong robustness for tracking sperm's motion under different microscopes. For assessment of the motility parameters, our system can work at a lower cost with a plastic structure. This system with DF imaging is suitable for portable POCT of sperm quality analysis, which is highly cost-effective in resource-constrained circumstances.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141880163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a biocompatible 3D hydrogel scaffold using continuous liquid interface production for the delivery of cell therapies to treat recurrent glioblastoma 利用连续液体界面生产技术开发生物相容性三维水凝胶支架,用于递送细胞疗法治疗复发性胶质母细胞瘤
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-30 DOI: 10.1002/btm2.10676
Lauren Kass, Morrent Thang, Yu Zhang, Cathleen DeVane, Julia Logan, Addis Tessema, Jillian Perry, Shawn Hingtgen

Glioblastoma (GBM) is the most common primary malignant brain tumor diagnosed in adults, carrying with it an extremely poor prognosis and limited options for effective treatment. Various cell therapies have emerged as promising candidates for GBM treatment but fail in the clinic due to poor tumor trafficking, poor transplantation efficiency, and high systemic toxicity. In this study, we design, characterize, and test a 3D-printed cell delivery platform that can enhance the survival of therapeutic cells implanted in the GBM resection cavity. Using continuous liquid interface production (CLIP) to generate a biocompatible 3D hydrogel, we demonstrate that we can effectively seed neural stem cells (NSCs) onto the surface of the hydrogel, and that the cells can proliferate to high densities when cultured for 14 days in vitro. We show that NSCs seeded on CLIP scaffolds persist longer than freely injected cells in vivo, proliferating to 20% higher than their original density in 6 days after implantation. Finally, we demonstrate that therapeutic fibroblasts seeded on CLIP more effectively suppress tumor growth and extend survival in a mouse model of LN229 GBM resection compared to the scaffold or therapeutic cells alone. These promising results demonstrate the potential to leverage CLIP to design hydrogels with various features to control the delivery of different types of cell therapies. Future work will include a more thorough evaluation of the immunological response to the material and improvement of the printing resolution for biocompatible aqueous resins.

胶质母细胞瘤(GBM)是成人中最常见的原发性恶性脑肿瘤,预后极差,有效治疗方案有限。各种细胞疗法已成为治疗 GBM 的有希望的候选疗法,但由于肿瘤贩运能力差、移植效率低和全身毒性大等原因,这些疗法在临床上均告失败。在本研究中,我们设计、表征并测试了一种三维打印细胞递送平台,该平台可提高植入 GBM 切除腔的治疗细胞的存活率。我们利用连续液态界面生产(CLIP)技术生成了一种生物相容性三维水凝胶,并证明我们能有效地将神经干细胞(NSCs)播种到水凝胶表面,而且细胞在体外培养 14 天后能高密度增殖。我们的研究表明,在 CLIP 支架上播种的神经干细胞比自由注射的细胞在体内存活时间更长,植入 6 天后,其增殖密度比原来高出 20%。最后,我们证明,在小鼠 LN229 GBM 切除模型中,与单独使用支架或治疗细胞相比,CLIP 上播种的治疗成纤维细胞能更有效地抑制肿瘤生长并延长存活时间。这些充满希望的结果表明,利用 CLIP 设计具有各种特性的水凝胶,以控制不同类型细胞疗法的递送是很有潜力的。未来的工作将包括对材料的免疫反应进行更全面的评估,以及提高生物相容性水性树脂的打印分辨率。
{"title":"Development of a biocompatible 3D hydrogel scaffold using continuous liquid interface production for the delivery of cell therapies to treat recurrent glioblastoma","authors":"Lauren Kass,&nbsp;Morrent Thang,&nbsp;Yu Zhang,&nbsp;Cathleen DeVane,&nbsp;Julia Logan,&nbsp;Addis Tessema,&nbsp;Jillian Perry,&nbsp;Shawn Hingtgen","doi":"10.1002/btm2.10676","DOIUrl":"10.1002/btm2.10676","url":null,"abstract":"<p>Glioblastoma (GBM) is the most common primary malignant brain tumor diagnosed in adults, carrying with it an extremely poor prognosis and limited options for effective treatment. Various cell therapies have emerged as promising candidates for GBM treatment but fail in the clinic due to poor tumor trafficking, poor transplantation efficiency, and high systemic toxicity. In this study, we design, characterize, and test a 3D-printed cell delivery platform that can enhance the survival of therapeutic cells implanted in the GBM resection cavity. Using continuous liquid interface production (CLIP) to generate a biocompatible 3D hydrogel, we demonstrate that we can effectively seed neural stem cells (NSCs) onto the surface of the hydrogel, and that the cells can proliferate to high densities when cultured for 14 days <i>in vitro</i>. We show that NSCs seeded on CLIP scaffolds persist longer than freely injected cells in vivo, proliferating to 20% higher than their original density in 6 days after implantation. Finally, we demonstrate that therapeutic fibroblasts seeded on CLIP more effectively suppress tumor growth and extend survival in a mouse model of LN229 GBM resection compared to the scaffold or therapeutic cells alone. These promising results demonstrate the potential to leverage CLIP to design hydrogels with various features to control the delivery of different types of cell therapies. Future work will include a more thorough evaluation of the immunological response to the material and improvement of the printing resolution for biocompatible aqueous resins.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141862171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The search for an optimal tissue-engineered urethra model for clinical application based on preclinical trials in male animals: A systematic review and meta-analysis 基于雄性动物的临床前试验,为临床应用寻找最佳组织工程尿道模型:系统回顾与荟萃分析
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-23 DOI: 10.1002/btm2.10700
Natalia Chepelova, Guzel Sagitova, Daniel Munblit, Aleksandr Suvorov, Andrey Morozov, Anastasia Shpichka, Peter Glybochko, Peter Timashev, Denis Butnaru

Tissue engineering has emerged as a promising avenue for reconstructive urology, though only a limited number of tissue-engineered urethral constructs have advanced to clinical testing. Presently, there exists a dearth of agreement regarding the most promising constructs deserving of implementation in clinical practice. The objective of this review was to provide a comprehensive analysis of preclinical trials findings of a tissue-engineered urethra and to identify the most promising constructs for future translation into clinical practice. A systematic search of the Pubmed, Scopus, and PMC databases was conducted in accordance with the PRISMA statement. Manuscripts published in English between 2015 and 2022, reporting on the methodology for creating a tissue-engineered urethra, assessing the regenerative potential of the scaffold in a male animal model, and evaluating the clinical and histological outcomes of treatment, were included. A total of 48 manuscripts met the inclusion criteria, with 12 being eligible for meta-analysis. Meta-analysis revealed no significant benefit of any matrix type in terms of complication rates. However, acellular matrices demonstrated significant advantage over cellular matrices in case of no postoperative stricture formation (odds ratio = 0.06 [95% CI 0.01; 0.23], p < 0.01). Among all subgroups (animal models and scaffold types), the usage of acellular matrices resulted in advantageous effects. The meta-regression analysis did not show a significant impact of defect length (β1 = −0.02 [−0.28; 0.23], p = 0.86). We found that decellularized materials may carry less relevance for urethral reconstruction due to unfavorable preclinical outcomes. Natural polymers, used independently or with synthetic materials, resulted in better postoperative outcomes in animals compared to purely synthetic constructs. Acellular scaffolds showed promising outcomes, matching or exceeding cellular constructs. However, more studies are needed to confirm their clinical effectiveness.

组织工程学已成为泌尿外科重建的一条前景广阔的途径,但只有少数组织工程尿道构建物已进入临床试验阶段。目前,关于最有希望应用于临床实践的构建物还缺乏一致意见。本综述的目的是对组织工程尿道的临床前试验结果进行全面分析,并确定最有希望在未来应用于临床实践的结构。根据 PRISMA 声明,对 Pubmed、Scopus 和 PMC 数据库进行了系统检索。纳入了 2015 年至 2022 年间发表的英文稿件,这些稿件报道了创建组织工程尿道的方法、评估支架在雄性动物模型中的再生潜力以及评估治疗的临床和组织学结果。共有 48 篇手稿符合纳入标准,其中 12 篇符合荟萃分析条件。荟萃分析表明,就并发症发生率而言,任何基质类型都没有明显的优势。不过,在术后不形成狭窄的情况下,无细胞基质比细胞基质有明显优势(几率比=0.06 [95% CI 0.01; 0.23],p <0.01)。在所有亚组(动物模型和支架类型)中,使用无细胞基质都会产生有利影响。元回归分析并未显示缺损长度的显著影响(β1 = -0.02 [-0.28; 0.23], p = 0.86)。我们发现,由于临床前研究结果不理想,脱细胞材料对尿道重建的意义可能较小。与纯合成材料相比,独立使用或与合成材料一起使用的天然聚合物可为动物带来更好的术后效果。细胞支架显示出良好的效果,可与细胞构建物媲美或超越细胞构建物。不过,还需要更多的研究来证实其临床效果。
{"title":"The search for an optimal tissue-engineered urethra model for clinical application based on preclinical trials in male animals: A systematic review and meta-analysis","authors":"Natalia Chepelova,&nbsp;Guzel Sagitova,&nbsp;Daniel Munblit,&nbsp;Aleksandr Suvorov,&nbsp;Andrey Morozov,&nbsp;Anastasia Shpichka,&nbsp;Peter Glybochko,&nbsp;Peter Timashev,&nbsp;Denis Butnaru","doi":"10.1002/btm2.10700","DOIUrl":"10.1002/btm2.10700","url":null,"abstract":"<p>Tissue engineering has emerged as a promising avenue for reconstructive urology, though only a limited number of tissue-engineered urethral constructs have advanced to clinical testing. Presently, there exists a dearth of agreement regarding the most promising constructs deserving of implementation in clinical practice. The objective of this review was to provide a comprehensive analysis of preclinical trials findings of a tissue-engineered urethra and to identify the most promising constructs for future translation into clinical practice. A systematic search of the Pubmed, Scopus, and PMC databases was conducted in accordance with the PRISMA statement. Manuscripts published in English between 2015 and 2022, reporting on the methodology for creating a tissue-engineered urethra, assessing the regenerative potential of the scaffold in a male animal model, and evaluating the clinical and histological outcomes of treatment, were included. A total of 48 manuscripts met the inclusion criteria, with 12 being eligible for meta-analysis. Meta-analysis revealed no significant benefit of any matrix type in terms of complication rates. However, acellular matrices demonstrated significant advantage over cellular matrices in case of no postoperative stricture formation (odds ratio = 0.06 [95% CI 0.01; 0.23], <i>p</i> &lt; 0.01). Among all subgroups (animal models and scaffold types), the usage of acellular matrices resulted in advantageous effects. The meta-regression analysis did not show a significant impact of defect length (β1 = −0.02 [−0.28; 0.23], <i>p</i> = 0.86). We found that decellularized materials may carry less relevance for urethral reconstruction due to unfavorable preclinical outcomes. Natural polymers, used independently or with synthetic materials, resulted in better postoperative outcomes in animals compared to purely synthetic constructs. Acellular scaffolds showed promising outcomes, matching or exceeding cellular constructs. However, more studies are needed to confirm their clinical effectiveness.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10700","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141755275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intra-lymph node crosslinking of antigen-bearing polymers enhances humoral immunity and dendritic cell activation 含抗原聚合物的淋巴结内交联可增强体液免疫和树突状细胞活化功能
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-17 DOI: 10.1002/btm2.10705
Erin M. Euliano, Anushka Agrawal, Marina H. Yu, Tyler P. Graf, Emily M. Henrich, Alyssa A. Kunkel, Chia-Chien Hsu, Tsvetelina Baryakova, Kevin J. McHugh

Lymph node (LN)-resident dendritic cells (DCs) are a promising target for vaccination given their professional antigen-presenting capabilities and proximity to a high concentration of immune cells. Direct intra-LN injection has been shown to greatly enhance the immune response to vaccine antigens compared to traditional intramuscular injection, but it is infeasible to implement clinically in a vaccination campaign context. Employing the passive lymphatic flow of antigens to target LNs has been shown to increase total antigen uptake by DCs more than inflammatory adjuvants, which recruit peripheral DCs. Herein, we describe a novel vaccination platform in which two complementary multi-arm poly(ethylene glycol) (PEG) polymers—one covalently bound to the model antigen ovalbumin (OVA)—are injected subcutaneously into two distinct sites. These materials then drain to the same LN through different lymphatic vessels and, upon meeting in the LN, rapidly crosslink. This system improves OVA delivery to, and residence time within, the draining LN compared to all control groups. The crosslinking of the two PEG components also improves humoral immunity without the need for any pathogen-mimicking adjuvants. Further, we observed a significant increase in non-B/T lymphocytes in LNs cross-presenting the OVA peptide SIINFEKL on MHC I over a dose-matched control containing alum, the most common clinical adjuvant, as well as an increase in DC activation in the LN. These data suggest that this platform can be used to deliver antigens to LN-resident immune cells to produce a stronger humoral and cellular immune response over materials-matched controls without the use of traditional adjuvants.

淋巴结(LN)驻留的树突状细胞(DC)具有专业的抗原递呈能力,而且靠近高浓度的免疫细胞,因此是一个很有前景的疫苗接种目标。与传统的肌肉注射相比,淋巴管内直接注射已被证明能大大提高对疫苗抗原的免疫反应,但在疫苗接种活动中临床应用却不可行。与招募外周直流细胞的炎性佐剂相比,利用抗原的被动淋巴流动来靶向LN更能提高直流细胞对抗原的总摄取量。在本文中,我们描述了一种新型疫苗接种平台,将两种互补的多臂聚(乙二醇)(PEG)聚合物--其中一种与模型抗原卵清蛋白(OVA)共价结合--皮下注射到两个不同的部位。然后,这些材料通过不同的淋巴管流向同一个淋巴结,在淋巴结中相遇后迅速交联。与所有对照组相比,该系统可改善 OVA 在引流 LN 中的输送和停留时间。两种 PEG 成分的交联还能提高体液免疫,而无需使用任何病原体模拟佐剂。此外,我们还观察到,与含有明矾(最常见的临床佐剂)的剂量匹配对照组相比,交叉呈现 MHC I 上 OVA 肽 SIINFEKL 的 LN 中的非 B/T 淋巴细胞明显增加,LN 中的 DC 激活也有所增加。这些数据表明,该平台可用于向LN驻留免疫细胞递送抗原,从而产生比材料匹配对照组更强的体液和细胞免疫反应,而无需使用传统佐剂。
{"title":"Intra-lymph node crosslinking of antigen-bearing polymers enhances humoral immunity and dendritic cell activation","authors":"Erin M. Euliano,&nbsp;Anushka Agrawal,&nbsp;Marina H. Yu,&nbsp;Tyler P. Graf,&nbsp;Emily M. Henrich,&nbsp;Alyssa A. Kunkel,&nbsp;Chia-Chien Hsu,&nbsp;Tsvetelina Baryakova,&nbsp;Kevin J. McHugh","doi":"10.1002/btm2.10705","DOIUrl":"10.1002/btm2.10705","url":null,"abstract":"<p>Lymph node (LN)-resident dendritic cells (DCs) are a promising target for vaccination given their professional antigen-presenting capabilities and proximity to a high concentration of immune cells. Direct intra-LN injection has been shown to greatly enhance the immune response to vaccine antigens compared to traditional intramuscular injection, but it is infeasible to implement clinically in a vaccination campaign context. Employing the passive lymphatic flow of antigens to target LNs has been shown to increase total antigen uptake by DCs more than inflammatory adjuvants, which recruit peripheral DCs. Herein, we describe a novel vaccination platform in which two complementary multi-arm poly(ethylene glycol) (PEG) polymers—one covalently bound to the model antigen ovalbumin (OVA)—are injected subcutaneously into two distinct sites. These materials then drain to the same LN through different lymphatic vessels and, upon meeting in the LN, rapidly crosslink. This system improves OVA delivery to, and residence time within, the draining LN compared to all control groups. The crosslinking of the two PEG components also improves humoral immunity without the need for any pathogen-mimicking adjuvants. Further, we observed a significant increase in non-B/T lymphocytes in LNs cross-presenting the OVA peptide SIINFEKL on MHC I over a dose-matched control containing alum, the most common clinical adjuvant, as well as an increase in DC activation in the LN. These data suggest that this platform can be used to deliver antigens to LN-resident immune cells to produce a stronger humoral and cellular immune response over materials-matched controls without the use of traditional adjuvants.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Matrix metalloproteinase 2-responsive dual-drug-loaded self-assembling peptides suppress tumor growth and enhance breast cancer therapy 基质金属蛋白酶 2 响应型双药自组装肽可抑制肿瘤生长并提高乳腺癌治疗效果
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-17 DOI: 10.1002/btm2.10702
Jihong Ma, Haiyan Yang, Xue Tian, Fanhu Meng, Xiaoqing Zhai, Aimei Li, Chuntao Li, Min Wang, Guohui Wang, Chunbo Lu, Jingkun Bai

Conventional chemotherapeutic agents are limited by their lack of targeting and penetration and their short retention time, and chemotherapy might induce an immune suppressive environment. Peptide self-assembly can result in a specific morphology, and the resulting morphological changes are stimuli responsive to the external environment, which is important for drug permeation and retention of encapsulated chemotherapeutic agents. In this study, a polypeptide (Pep1) containing the peptide sequences PLGLAG and RGD that is responsive to matrix metalloproteinase 2 (MMP-2) was successfully developed. Pep1 underwent a morphological transformation from a spherical structure to aggregates with a high aspect ratio in response to MMP-2 induction. This drug delivery system (DI/Pep1) can transport doxorubicin (DOX) and indomethacin (IND) simultaneously to target tumor cells for subsequent drug release while extending drug retention within tumor cells, which increases immunogenic cell death and facilitates the immunotherapeutic effect of CD4+ T cells. Ultimately, DI/Pep1 attenuated tumor-associated inflammation, enhanced the body's immune response, and inhibited breast cancer growth by combining the actions of DOX and IND. Our research offers an approach to hopefully enhance the effectiveness of cancer treatment.

传统的化疗药物由于缺乏靶向性和穿透性以及保留时间短而受到限制,而且化疗可能会诱发免疫抑制环境。多肽自组装可形成特定的形态,由此产生的形态变化是对外部环境的刺激反应,这对药物渗透和包封化疗药物的保留非常重要。本研究成功开发了一种多肽(Pep1),它含有对基质金属蛋白酶 2(MMP-2)有反应的肽序列 PLGLAG 和 RGD。在 MMP-2 诱导下,Pep1 从球形结构形态转变为高纵横比的聚集体。这种给药系统(DI/Pep1)可将多柔比星(DOX)和吲哚美辛(IND)同时运送到靶肿瘤细胞,以便随后释放药物,同时延长药物在肿瘤细胞内的保留时间,从而增加免疫原性细胞死亡,促进 CD4+ T 细胞的免疫治疗效果。最终,DI/Pep1 通过结合 DOX 和 IND 的作用,减轻了肿瘤相关炎症,增强了机体的免疫反应,抑制了乳腺癌的生长。我们的研究提供了一种有望提高癌症治疗效果的方法。
{"title":"Matrix metalloproteinase 2-responsive dual-drug-loaded self-assembling peptides suppress tumor growth and enhance breast cancer therapy","authors":"Jihong Ma,&nbsp;Haiyan Yang,&nbsp;Xue Tian,&nbsp;Fanhu Meng,&nbsp;Xiaoqing Zhai,&nbsp;Aimei Li,&nbsp;Chuntao Li,&nbsp;Min Wang,&nbsp;Guohui Wang,&nbsp;Chunbo Lu,&nbsp;Jingkun Bai","doi":"10.1002/btm2.10702","DOIUrl":"10.1002/btm2.10702","url":null,"abstract":"<p>Conventional chemotherapeutic agents are limited by their lack of targeting and penetration and their short retention time, and chemotherapy might induce an immune suppressive environment. Peptide self-assembly can result in a specific morphology, and the resulting morphological changes are stimuli responsive to the external environment, which is important for drug permeation and retention of encapsulated chemotherapeutic agents. In this study, a polypeptide (Pep1) containing the peptide sequences PLGLAG and RGD that is responsive to matrix metalloproteinase 2 (MMP-2) was successfully developed. Pep1 underwent a morphological transformation from a spherical structure to aggregates with a high aspect ratio in response to MMP-2 induction. This drug delivery system (DI/Pep1) can transport doxorubicin (DOX) and indomethacin (IND) simultaneously to target tumor cells for subsequent drug release while extending drug retention within tumor cells, which increases immunogenic cell death and facilitates the immunotherapeutic effect of CD4<sup>+</sup> T cells. Ultimately, DI/Pep1 attenuated tumor-associated inflammation, enhanced the body's immune response, and inhibited breast cancer growth by combining the actions of DOX and IND. Our research offers an approach to hopefully enhance the effectiveness of cancer treatment.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnosis of pregnancy disorder in the first-trimester patient plasma with Raman spectroscopy and protein analysis 利用拉曼光谱和蛋白质分析诊断初产妇血浆中的妊娠紊乱症
IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-16 DOI: 10.1002/btm2.10691
Ansuja P. Mathew, Gabriel Cutshaw, Olivia Appel, Meghan Funk, Lilly Synan, Joshua Waite, Saman Ghazvini, Xiaona Wen, Soumik Sarkar, Mark Santillan, Donna Santillan, Rizia Bardhan

Gestational diabetes mellitus (GDM) is a pregnancy disorder associated with short- and long-term adverse outcomes in both mothers and infants. The current clinical test of blood glucose levels late in the second trimester is inadequate for early detection of GDM. Here we show the utility of Raman spectroscopy (RS) for rapid and highly sensitive maternal metabolome screening for GDM in the first trimester. Key metabolites, including phospholipids, carbohydrates, and major amino acids, were identified with RS and validated with mass spectrometry, enabling insights into associated metabolic pathway enrichment. Using classical machine learning (ML) approaches, we showed the performance of the RS metabolic model (cross-validation AUC 0.97) surpassed that achieved with patients' clinical data alone (cross-validation AUC 0.59) or prior studies with single biomarkers. Further, we analyzed novel proteins and identified fetuin-A as a promising candidate for early GDM prediction. A correlation analysis showed a moderate to strong correlation between multiple metabolites and proteins, suggesting a combined protein-metabolic analysis integrated with ML would enable a powerful screening platform for first trimester diagnosis. Our study underscores RS metabolic profiling as a cost-effective tool that can be integrated into the current clinical workflow for accurate risk stratification of GDM and to improve both maternal and neonatal outcomes.

妊娠糖尿病(GDM)是一种与母婴短期和长期不良后果相关的妊娠疾病。目前临床上对妊娠后期血糖水平的检测不足以早期发现 GDM。在这里,我们展示了拉曼光谱(RS)在妊娠头三个月快速、高灵敏地筛查 GDM 的母体代谢组的实用性。利用拉曼光谱鉴定了包括磷脂、碳水化合物和主要氨基酸在内的关键代谢物,并通过质谱分析进行了验证,从而了解了相关代谢途径的丰富程度。使用经典的机器学习(ML)方法,我们发现 RS 代谢模型的性能(交叉验证 AUC 0.97)超过了仅使用患者临床数据(交叉验证 AUC 0.59)或之前使用单一生物标记物进行的研究。此外,我们还分析了新型蛋白质,发现胎蛋白-A 是预测早期 GDM 的理想候选蛋白。相关性分析表明,多种代谢物与蛋白质之间存在中度到高度的相关性,这表明蛋白质代谢分析与 ML 的结合将为孕前三个月的诊断提供一个强大的筛查平台。我们的研究强调了 RS 代谢图谱分析是一种具有成本效益的工具,可以整合到当前的临床工作流程中,对 GDM 进行准确的风险分层,并改善孕产妇和新生儿的预后。
{"title":"Diagnosis of pregnancy disorder in the first-trimester patient plasma with Raman spectroscopy and protein analysis","authors":"Ansuja P. Mathew,&nbsp;Gabriel Cutshaw,&nbsp;Olivia Appel,&nbsp;Meghan Funk,&nbsp;Lilly Synan,&nbsp;Joshua Waite,&nbsp;Saman Ghazvini,&nbsp;Xiaona Wen,&nbsp;Soumik Sarkar,&nbsp;Mark Santillan,&nbsp;Donna Santillan,&nbsp;Rizia Bardhan","doi":"10.1002/btm2.10691","DOIUrl":"10.1002/btm2.10691","url":null,"abstract":"<p>Gestational diabetes mellitus (GDM) is a pregnancy disorder associated with short- and long-term adverse outcomes in both mothers and infants. The current clinical test of blood glucose levels late in the second trimester is inadequate for early detection of GDM. Here we show the utility of Raman spectroscopy (RS) for rapid and highly sensitive maternal metabolome screening for GDM in the first trimester. Key metabolites, including phospholipids, carbohydrates, and major amino acids, were identified with RS and validated with mass spectrometry, enabling insights into associated metabolic pathway enrichment. Using classical machine learning (ML) approaches, we showed the performance of the RS metabolic model (cross-validation AUC 0.97) surpassed that achieved with patients' clinical data alone (cross-validation AUC 0.59) or prior studies with single biomarkers. Further, we analyzed novel proteins and identified fetuin-A as a promising candidate for early GDM prediction. A correlation analysis showed a moderate to strong correlation between multiple metabolites and proteins, suggesting a combined protein-metabolic analysis integrated with ML would enable a powerful screening platform for first trimester diagnosis. Our study underscores RS metabolic profiling as a cost-effective tool that can be integrated into the current clinical workflow for accurate risk stratification of GDM and to improve both maternal and neonatal outcomes.</p>","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Bioengineering & Translational Medicine
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1