In Situ Green Synthesis of Co3O4@ZnO Core-Shell Nanoparticles Using Datura stramonium Leaf Extract: Antibacterial and Antioxidant Studies

IF 4.7 3区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioinorganic Chemistry and Applications Pub Date : 2023-11-29 DOI:10.1155/2023/5019838
Gezahegn Tadesse, H. C. Ananda Murthy, C. R. Ravikumar, T. Naveen Kumar, Lema Teshome, Tegene Desalegn
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In this study, Co<sub>3</sub>O<sub>4</sub>@ZnO core-shell nanoparticles (CZCS NPs) have been synthesized using three different core to shell materials ratios of Co<sub>3</sub>O<sub>4</sub> to ZnO (0.5 : 0.25 CZCS (1), 0.5 : 0.5 CZCS (2), and 0.5 : 0.75 M CZCS (3)) by employing <i>Datura stramonium</i> leaf extract. The polycrystalline nature of Co<sub>3</sub>O<sub>4</sub>@ZnO core-shell nanoparticles was investigated using the XRD and SAED characterization techniques. The investigated nanostructure of Co<sub>3</sub>O<sub>4</sub>@ZnO core-shell nanoparticles appeared with Co<sub>3</sub>O<sub>4</sub> as the core and ZnO as an outer shell. Additionally, a variety of physicochemical properties of the nanoparticles were determined using various characterization techniques. The average crystallite sizes of CZCS (1), CZCS (2), and CZCS (3) were found to be <span><svg height=\"8.69875pt\" style=\"vertical-align:-0.3499298pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 23.017 8.69875\" width=\"23.017pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,6.24,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,15.386,0)\"></path></g></svg><span></span><span><svg height=\"8.69875pt\" style=\"vertical-align:-0.3499298pt\" version=\"1.1\" viewbox=\"25.872183800000002 -8.34882 15.677 8.69875\" width=\"15.677pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,25.922,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,32.162,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,35.126,0)\"><use xlink:href=\"#g113-53\"></use></g></svg>,</span></span> <span><svg height=\"8.69875pt\" style=\"vertical-align:-0.3499298pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 23.017 8.69875\" width=\"23.017pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-51\"></use></g><g transform=\"matrix(.013,0,0,-0.013,6.24,0)\"><use xlink:href=\"#g113-51\"></use></g><g transform=\"matrix(.013,0,0,-0.013,15.386,0)\"><use xlink:href=\"#g117-37\"></use></g></svg><span></span><span><svg height=\"8.69875pt\" style=\"vertical-align:-0.3499298pt\" version=\"1.1\" viewbox=\"25.872183800000002 -8.34882 15.677 8.69875\" width=\"15.677pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,25.922,0)\"><use xlink:href=\"#g113-50\"></use></g><g transform=\"matrix(.013,0,0,-0.013,32.162,0)\"><use xlink:href=\"#g113-47\"></use></g><g transform=\"matrix(.013,0,0,-0.013,35.126,0)\"></path></g></svg>,</span></span> and <span><svg height=\"8.69875pt\" style=\"vertical-align:-0.3499298pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 23.017 8.69875\" width=\"23.017pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-51\"></use></g><g transform=\"matrix(.013,0,0,-0.013,6.24,0)\"><use xlink:href=\"#g113-54\"></use></g><g transform=\"matrix(.013,0,0,-0.013,15.386,0)\"><use xlink:href=\"#g117-37\"></use></g></svg><span></span><svg height=\"8.69875pt\" style=\"vertical-align:-0.3499298pt\" version=\"1.1\" viewbox=\"25.872183800000002 -8.34882 15.677 8.69875\" width=\"15.677pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,25.922,0)\"><use xlink:href=\"#g113-50\"></use></g><g transform=\"matrix(.013,0,0,-0.013,32.162,0)\"><use xlink:href=\"#g113-47\"></use></g><g transform=\"matrix(.013,0,0,-0.013,35.126,0)\"><use xlink:href=\"#g113-54\"></use></g></svg></span> nm, respectively. The band gap energy values for CZCS (1), CZCS (2), and CZCS (3) determined from the UV-DRS data were found to be 2.75, 2.76, and 2.73 eV, respectively. The high inhibition activities against <i>S. aureus</i>, <i>S. pyogenes</i>, <i>E. coli</i>, and <i>P. aeruginosa</i> bacterial strains were obtained for the small size CZCS (2) nanoparticles at the concentration of 100 mg/mL with 22 ± 0.34, 19 ± <span><svg height=\"8.55521pt\" style=\"vertical-align:-0.2063904pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 9.204 8.55521\" width=\"9.204pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,6.24,0)\"><use xlink:href=\"#g113-47\"></use></g></svg><span></span><span><svg height=\"8.55521pt\" style=\"vertical-align:-0.2063904pt\" version=\"1.1\" viewbox=\"9.1541838 -8.34882 12.661 8.55521\" width=\"12.661pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,9.204,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,15.444,0)\"><use xlink:href=\"#g113-51\"></use></g></svg>,</span></span> 18 ± <span><svg height=\"8.55521pt\" style=\"vertical-align:-0.2063904pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 21.8649 8.55521\" width=\"21.8649pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,6.24,0)\"><use xlink:href=\"#g113-47\"></use></g><g transform=\"matrix(.013,0,0,-0.013,9.204,0)\"><use xlink:href=\"#g113-53\"></use></g><g transform=\"matrix(.013,0,0,-0.013,15.444,0)\"><use xlink:href=\"#g113-54\"></use></g></svg>,</span> and 17 ± <span><svg height=\"8.55521pt\" style=\"vertical-align:-0.2063904pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.34882 6.36303 8.55521\" width=\"6.36303pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-49\"></use></g></svg>.</span>32 mm values, respectively. The high inhibition performance of CZCS (2) nanoparticles against Gram-positive and Gram-negative bacteria which is even above the control drug ampicillin is because of its small size and synergistic effect. The percentage scavenging activity of Co<sub>3</sub>O<sub>4</sub>@ZnO core-shell nanoparticles was also studied and CZCS (2) nanoparticles showed a good scavenging capacity (86.87%) at 500 <i>μ</i>g/mL with IC50 of 209.26 <i>μ</i>g/mL.","PeriodicalId":8914,"journal":{"name":"Bioinorganic Chemistry and Applications","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioinorganic Chemistry and Applications","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1155/2023/5019838","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Investigating and synthesizing potent antibacterial NPs using biological methods is highly preferred, and it involves nontoxic, cost-effective, and environmentally friendly chemicals and methods. Antibiotic drug resistance and oxidative stress have become a serious public health issue worldwide. Hence, the key objective of this study was to biologically synthesize and characterize the potent antibacterial Co3O4@ZnO core-shell nanoparticles for the antibacterial application. The radical scavenging ability of green synthesized Co3O4@ZnO core-shell nanoparticles was also determined. In this study, Co3O4@ZnO core-shell nanoparticles (CZCS NPs) have been synthesized using three different core to shell materials ratios of Co3O4 to ZnO (0.5 : 0.25 CZCS (1), 0.5 : 0.5 CZCS (2), and 0.5 : 0.75 M CZCS (3)) by employing Datura stramonium leaf extract. The polycrystalline nature of Co3O4@ZnO core-shell nanoparticles was investigated using the XRD and SAED characterization techniques. The investigated nanostructure of Co3O4@ZnO core-shell nanoparticles appeared with Co3O4 as the core and ZnO as an outer shell. Additionally, a variety of physicochemical properties of the nanoparticles were determined using various characterization techniques. The average crystallite sizes of CZCS (1), CZCS (2), and CZCS (3) were found to be , , and  nm, respectively. The band gap energy values for CZCS (1), CZCS (2), and CZCS (3) determined from the UV-DRS data were found to be 2.75, 2.76, and 2.73 eV, respectively. The high inhibition activities against S. aureus, S. pyogenes, E. coli, and P. aeruginosa bacterial strains were obtained for the small size CZCS (2) nanoparticles at the concentration of 100 mg/mL with 22 ± 0.34, 19 ± , 18 ± , and 17 ± .32 mm values, respectively. The high inhibition performance of CZCS (2) nanoparticles against Gram-positive and Gram-negative bacteria which is even above the control drug ampicillin is because of its small size and synergistic effect. The percentage scavenging activity of Co3O4@ZnO core-shell nanoparticles was also studied and CZCS (2) nanoparticles showed a good scavenging capacity (86.87%) at 500 μg/mL with IC50 of 209.26 μg/mL.
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利用曼陀罗叶提取物原位绿色合成Co3O4@ZnO核壳纳米颗粒:抗菌和抗氧化研究
使用生物方法研究和合成有效的抗菌NPs是高度优选的,它涉及无毒,成本效益和环境友好的化学品和方法。抗生素耐药和氧化应激已成为世界范围内严重的公共卫生问题。因此,本研究的主要目的是生物学合成和表征高效抗菌Co3O4@ZnO核壳纳米颗粒,用于抗菌应用。同时测定了绿色合成Co3O4@ZnO核壳纳米粒子的自由基清除能力。本研究以曼陀罗叶提取物为原料,采用Co3O4与ZnO三种不同芯壳比(0.5:0.25 CZCS(1)、0.5:0.5 CZCS(2)和0.5:0.75 M CZCS(3))制备了Co3O4@ZnO核壳纳米粒子(CZCS NPs)。利用XRD和SAED表征技术研究了Co3O4@ZnO核壳纳米颗粒的多晶性质。研究得到了以Co3O4为核心,ZnO为外壳的Co3O4@ZnO核壳纳米粒子结构。此外,使用各种表征技术确定了纳米颗粒的各种物理化学性质。CZCS(1)、CZCS(2)和CZCS(3)的平均晶粒尺寸分别为、和nm。通过UV-DRS数据测定CZCS(1)、CZCS(2)和CZCS(3)的带隙能值分别为2.75、2.76和2.73 eV。小粒径CZCS(2)纳米颗粒在浓度为100 mg/mL时,分别为22±0.34、19±、18±和17±,对金黄色葡萄球菌、化脓性葡萄球菌、大肠杆菌和铜绿假单胞菌具有较高的抑制活性。分别为32mm值。CZCS(2)纳米颗粒对革兰氏阳性菌和革兰氏阴性菌的抑制性能甚至高于对照药物氨苄西林,这是由于其体积小且具有协同作用。研究了Co3O4@ZnO核壳纳米粒子的百分比清除率,结果表明,CZCS(2)纳米粒子在500 μg/mL时清除率为86.87%,IC50为209.26 μg/mL。
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来源期刊
Bioinorganic Chemistry and Applications
Bioinorganic Chemistry and Applications 化学-生化与分子生物学
CiteScore
7.00
自引率
5.30%
发文量
105
审稿时长
>12 weeks
期刊介绍: Bioinorganic Chemistry and Applications is primarily devoted to original research papers, but also publishes review articles, editorials, and letter to the editor in the general field of bioinorganic chemistry and its applications. Its scope includes all aspects of bioinorganic chemistry, including bioorganometallic chemistry and applied bioinorganic chemistry. The journal welcomes papers relating to metalloenzymes and model compounds, metal-based drugs, biomaterials, biocatalysis and bioelectronics, metals in biology and medicine, metals toxicology and metals in the environment, metal interactions with biomolecules and spectroscopic applications.
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