Rapid and sensitive screening system of Mycoplasma hyorhinis based on Sandwich-DNA hybridization with nano-amplifiers

IF 8.1 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Materials Today Advances Pub Date : 2023-11-07 DOI:10.1016/j.mtadv.2023.100437

Audrey Wang, Xindan Zhang, Haoran Shi, Youngkwan Lee, Zhenqi Jiang, Soochan Kim, Jieling Qin

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Abstract

Mycoplasma contamination in cell/tissue cultures significantly affects cell characteristics, susceptibility to infectious pathogens, and drug reactions without obvious morphological changes and noticeable changes in cell growth rates. Although the technology for screening mycoplasma is essential, conventional technologies have limitations in the selection of a quick and easy manner. Here, we introduce an innovative screening system for mycoplasma based on electrochemical methods. In the electrochemical screening, a newly designed electrode, consisting of a sandwich-DNA hybridization for high selectivity and a nano-amplifier to improve the sensitivity, has implemented a realistic screening system by presenting detection capability at extremely low (ng/μL) levels and detection potential in real samples. Therefore, this system offers rapid screening, data accuracy, temporal effectiveness, and affordable-and-portable installation, enabling timely elimination of Mycoplasma contamination and facilitating progress in biomedical and pharmaceutical research.

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基于纳米扩增夹心dna杂交的缩支原体快速灵敏筛选系统

在细胞/组织培养中，支原体污染显著影响细胞特性、对感染性病原体的易感性和药物反应，但没有明显的形态学改变和细胞生长速率的明显变化。虽然支原体筛选技术是必不可少的，但传统技术在选择快速简便的方法方面存在局限性。本文介绍了一种新型的基于电化学方法的支原体筛选系统。在电化学筛选中，新设计的电极由高选择性的三明治- dna杂交和提高灵敏度的纳米放大器组成，具有极低(ng/μL)水平的检测能力和对实际样品的检测潜力，实现了一个现实的筛选系统。因此，该系统具有快速筛选、数据准确性、时效性以及价格合理、便于携带的特点，能够及时消除支原体污染，促进生物医学和制药研究的进展。

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来源期刊

Materials Today Advances MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

14.30

自引率

2.00%

发文量

116

审稿时长

32 days

期刊介绍： Materials Today Advances is a multi-disciplinary, open access journal that aims to connect different communities within materials science. It covers all aspects of materials science and related disciplines, including fundamental and applied research. The focus is on studies with broad impact that can cross traditional subject boundaries. The journal welcomes the submissions of articles at the forefront of materials science, advancing the field. It is part of the Materials Today family and offers authors rigorous peer review, rapid decisions, and high visibility.