De novo transcriptome assembly reveals characteristics of flower sex determination of Excoecaria agallocha

IF 2.5 3区农林科学 Q1 FORESTRY Annals of Forest Science Pub Date : 2022-08-24 DOI:10.1186/s13595-022-01156-6

Yan Zhou, Lulu Hao, Lexiang Huang, Xiaoming Tang, Danting Zhuo, Li Yun Wang, Ying Zhang

{"title":"De novo transcriptome assembly reveals characteristics of flower sex determination of Excoecaria agallocha","authors":"Yan Zhou, Lulu Hao, Lexiang Huang, Xiaoming Tang, Danting Zhuo, Li Yun Wang, Ying Zhang","doi":"10.1186/s13595-022-01156-6","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Key message</h3><p>MADS-box genes family may play important roles in the flower sex determination in <i>Excoecaria agallocha</i>. A total of 73 MADS-box genes were identified in <i>E. agallocha</i>. De novo transcriptome assembly analysis suggested that <i>AP1/FUL</i>, <i>AP3/PI</i>, <i>AGL104</i>, and <i>SOC1</i> plays potential roles in <i>E. agallocha</i> flower sex determination.</p><h3 data-test=\"abstract-sub-heading\">Context</h3><p><i>Excoecaria agallocha</i> is a dioecious species containing both male and female individuals producing unisexual flowers. The underlying mechanisms regulating inflorescence differentiation in these species remains poorly understood.</p><h3 data-test=\"abstract-sub-heading\">Aims</h3><p>Flower development influences reproduction and breeding in <i>E.agallocha</i>, which contributes to ecological restoration in the intertidal zone.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>We performed de novo transcriptome assembly analysis on male and female flowers and leaves from <i>E. agallocha</i>.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>We identified a total of 73 MADS-box genes in the <i>E. agallocha</i> genome, which we grouped into five distinct classes (MIKC<sup>c</sup>, Mα, Mβ, Mγ, MIKC*) after phylogenetic comparisons with <i>J. curcas</i> homologs. We analyzed expression profiles and suggested <i>AP1/FUL</i>, <i>AP3/PI</i>, <i>AGL104</i>, and <i>SOC1</i> as candidate regulators of sex determination. In addition, several <i>EaMADS</i> genes were significantly upregulated in flowers compared to leaves.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>Our study represents the first detailed, comprehensive evaluation of the regulation of MADS-box genes associated with sex determination in <i>E. agallocha</i>. The assembled transcriptomic profiles increase the genetic information available for this species and constitute an important contribution to functional studies of inflorescence genes. In turn, this will help exploring the molecular mechanisms underlying the evolution of <i>E. agallocha</i>.</p>","PeriodicalId":7994,"journal":{"name":"Annals of Forest Science","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Forest Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s13595-022-01156-6","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}

引用次数: 0

Abstract

Key message

MADS-box genes family may play important roles in the flower sex determination in Excoecaria agallocha. A total of 73 MADS-box genes were identified in E. agallocha. De novo transcriptome assembly analysis suggested that AP1/FUL, AP3/PI, AGL104, and SOC1 plays potential roles in E. agallocha flower sex determination.

Context

Excoecaria agallocha is a dioecious species containing both male and female individuals producing unisexual flowers. The underlying mechanisms regulating inflorescence differentiation in these species remains poorly understood.

Aims

Flower development influences reproduction and breeding in E.agallocha, which contributes to ecological restoration in the intertidal zone.

Methods

We performed de novo transcriptome assembly analysis on male and female flowers and leaves from E. agallocha.

Results

We identified a total of 73 MADS-box genes in the E. agallocha genome, which we grouped into five distinct classes (MIKC^c, Mα, Mβ, Mγ, MIKC*) after phylogenetic comparisons with J. curcas homologs. We analyzed expression profiles and suggested AP1/FUL, AP3/PI, AGL104, and SOC1 as candidate regulators of sex determination. In addition, several EaMADS genes were significantly upregulated in flowers compared to leaves.

Conclusion

Our study represents the first detailed, comprehensive evaluation of the regulation of MADS-box genes associated with sex determination in E. agallocha. The assembled transcriptomic profiles increase the genetic information available for this species and constitute an important contribution to functional studies of inflorescence genes. In turn, this will help exploring the molecular mechanisms underlying the evolution of E. agallocha.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

从头转录组组装揭示了花性决定的特征

关键信息emads -box基因家族可能在花性别决定中起重要作用。鉴定出73个MADS-box基因。从头转录组组装分析表明，AP1/FUL、AP3/PI、AGL104和SOC1可能在沉香花性别决定中发挥作用。沉香果是雌雄异株种，含有雄性和雌性个体，产生单性花。调控这些物种花序分化的潜在机制仍然知之甚少。目的花的发育影响着潮间带的生殖和繁殖，为潮间带生态恢复做出贡献。方法对沉香花雌雄花和叶片进行从头转录组组装分析。结果通过与麻瓜同源基因的系统发育比较，共鉴定出73个MADS-box基因，并将其分为MIKCc、Mα、Mβ、Mγ、MIKC* 5类。我们分析了表达谱，并建议AP1/FUL、AP3/PI、AGL104和SOC1作为性别决定的候选调节因子。此外，与叶片相比，几个EaMADS基因在花中显著上调。结论本研究首次详细、全面地评价了与性别决定相关的MADS-box基因的调控。组装的转录组谱增加了该物种的遗传信息，并对花序基因的功能研究做出了重要贡献。反过来，这将有助于探索E. agallocha进化的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Annals of Forest Science 农林科学-林学

CiteScore

6.70

自引率

3.30%

发文量

审稿时长

12-24 weeks

期刊介绍： Annals of Forest Science is an official publication of the French National Institute for Agriculture, Food and Environment (INRAE) -Up-to-date coverage of current developments and trends in forest research and forestry Topics include ecology and ecophysiology, genetics and improvement, tree physiology, wood quality, and silviculture -Formerly known as Annales des Sciences Forestières -Biology of trees and associated organisms (symbionts, pathogens, pests) -Forest dynamics and ecosystem processes under environmental or management drivers (ecology, genetics) -Risks and disturbances affecting forest ecosystems (biology, ecology, economics) -Forestry wood chain (tree breeding, forest management and productivity, ecosystem services, silviculture and plantation management) -Wood sciences (relationships between wood structure and tree functions, and between forest management or environment and wood properties)