Honeyberry-derived carbon quantum dots ameliorate LPS-induced neuroinflammation and oxidative stress through Nrf2/HO-1 signalling in HMC3 cells

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2023-02-22 DOI:10.1080/21691401.2023.2179062

Sanjay, Anshul Sharma, Hae-Jeung Lee

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引用次数: 1

Abstract

Carbon quantum dots (CQDs) were synthesized from blue honeysuckle (Lonicera caerulea) berry fruit extracts using a well-known, cost-effective, and environmental friendly hydrothermal process. The material was characterized using UV-vis spectroscopy, photoluminescence (PL), XPS, and TEM studies. The as-synthesized carbon dots exhibit excellent PL properties, with a quantum yield of ∼35.92%. CQDs vary in size from ∼2 nm to 9 nm. This study established the neuroprotective effects of CQDs against lipopolysaccharide (LPS)-induced human microglial cell model. LPS was found to induce cytotoxicity, reactive oxygen species, and pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and tumour necrosis factor-α) and downregulated enzymatic antioxidants such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2), superoxide dismutase, catalase, haem oxygenase (HO)-1, HO-2, and glutathione peroxidase, while CQDs treatment reversed LPS induced cytotoxicity, induced anti-inflammatory cytokines (IL-4, IL-10, and transforming growth factor β) and induce enzymatic antioxidants both at transcriptional and translational levels. The study suggested the potential role of CQDs prepared from Lonicera caerulea, as anti-inflammatory and antioxidative agents in neuroinflammatory and neurodegenerative diseases. In addition, CQDs could be exploited in various biomedical applications such as biosensing, drug delivery and tissue engineering.

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蜂蜜衍生的碳量子点通过Nrf2/HO-1信号传导改善lps诱导的HMC3细胞的神经炎症和氧化应激

摘要以蓝金银花(Lonicera caerulea)浆果提取物为原料，采用水热法合成碳量子点(CQDs)。利用紫外可见光谱、光致发光(PL)、XPS和TEM对材料进行了表征。合成的碳点具有优异的PL性能，量子产率为~ 35.92%。cqd的尺寸从~ 2nm到9nm不等。本研究建立了CQDs对脂多糖(LPS)诱导的人小胶质细胞模型的神经保护作用。研究发现，LPS可诱导细胞毒性、活性氧、促炎细胞因子白介素(IL)-1β、IL-6和肿瘤坏死因子-α，并下调核因子-红因子-2相关因子2 (Nrf2)、超氧化物歧化酶、过氧化氢酶、血红素加氧酶(HO)-1、HO-2和谷胱甘肽过氧化物酶等酶促抗氧化剂，而CQDs可逆转LPS诱导的细胞毒性、诱导抗炎细胞因子(IL-4、IL-10、IL-10)。和转化生长因子β)，并在转录和翻译水平诱导酶抗氧化剂。提示金银花CQDs在神经炎症和神经退行性疾病中具有抗炎和抗氧化作用。此外，CQDs还可用于生物传感、药物传递和组织工程等多种生物医学应用。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.