Probiotic Features of Three Lactic Acid Bacteria Strains Isolated from Foods and Characterization of Their Bioactive Molecules

IF 1.3 4区生物学 Q4 MICROBIOLOGY Microbiology Pub Date : 2023-11-28 DOI:10.1134/s0026261722603554

S. El oirdi, T. Lakhlifi, V. Fraberger, S. D’Amico, A. C. Kaddouri, A. Bouymajane, M. Yatim, A. Belhaj

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Abstract

New probiotic strains of lactic acid bacteria (LAB) are needed to meet the increasing consumer demand for probiotics. Hence, the aim of the present study was to investigate the probiotic properties of three LAB strains, namely Lactiplantibacillus plantarum 4F, Lactiplantibacillus plantarum 4JC, and Lacticaseibacillus paracasei 3MI3 using several in vitro probiotic tests. Results revealed that all strains showed high survival rate (≥60%) under extreme conditions of the gastrointestinal tract (lysozyme, pepsin, and bile). They were also able to auto-aggregate and co-aggregate with different pathogens. Moreover, all strains exhibited high antioxidant activity and antimicrobial ability against nine pathogens (Salmonella typhimurium, Listeria monocytogenes, Klebsiella pneumonia, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, Penicilium brasilianum, Aspergillus niger, and Fusarium oxysporum). HPLC and GC-MS analysis revealed the involvement of various organic and fatty acids in the observed antibacterial and antifungal activities. Lactic acid was the main produced compound with a significant concentration of 17.11 g/L. The biosafety assessment indicated that these strains were susceptible to most tested antibiotics, with no hemolytic and DNase activities. These findings reveal that the three studied strains of lactic acid bacteria have the potential to serve as novel probiotics in the development of functional products that promote health benefits.

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从食品中分离的三株乳酸菌的益生菌特性及其生物活性分子的鉴定

摘要为了满足消费者对益生菌日益增长的需求，需要新的乳酸菌(LAB)菌株。因此，本研究的目的是通过几种体外益生菌试验来研究三种乳酸菌菌株，即植物乳酸杆菌4F、植物乳酸杆菌4JC和副干酪乳杆菌3MI3的益生菌特性。结果显示，所有菌株在胃肠道(溶菌酶、胃蛋白酶和胆汁)的极端条件下均表现出较高的存活率(≥60%)。它们还能够与不同的病原体自动聚集或共同聚集。此外，所有菌株对9种病原菌(鼠伤寒沙门菌、单核增生李斯特菌、肺炎克雷伯菌、大肠埃希菌、鲍曼不动杆菌、铜绿假单胞菌、巴西青霉、黑曲霉和尖孢镰刀菌)均表现出较高的抗氧化活性和抗菌能力。HPLC和GC-MS分析显示多种有机酸和脂肪酸参与了抗菌和抗真菌活性。乳酸为主要产物，浓度为17.11 g/L。生物安全性评价表明，这些菌株对大多数抗生素敏感，没有溶血和dna酶活性。这些发现表明，所研究的三种乳酸菌菌株具有作为新型益生菌的潜力，可用于开发促进健康的功能性产品。

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来源期刊

Microbiology 生物-微生物学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.