Transcriptomic-based roadmap to the healthy and ozone-exposed lung

Philip Moos, Jenna Cheminant, Ujjwal Adhikari, Alessandro Venosa
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Abstract

The lung is constantly exposed to a myriad of exogenous stressors. Ground-level ozone represents a ubiquitous and extremely reactive anthropogenic toxicant, impacting the health of millions across the globe. While abundant, epidemiological, in vivo, and in vitro data focuses the ozone toxicity in individual cell types (e.g. epithelial type II, alveolar macrophages) or signaling pathways involved in the injury (e.g. akt, glutathione). When appropriately used, bulk and single-cell RNA sequencing techniques have the potential to provide complete, and in certain cases unbiased, information of the molecular events taking place in the steady-state and injured lung, and even capture the phenotypic diversity of neighboring cells. To this end, this review compiles information pertaining to the latest understanding of lung cell identity and activation in the steady-state and ozone-exposed lung. In addition, it discusses the value and benefits of multi-omics approaches and other tools developed to predict cell–cell communication and dissect spatial heterogeneity.

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基于转录组学的健康和臭氧暴露肺路线图
肺经常暴露在无数的外源压力下。地面臭氧是一种普遍存在的、极具活性的人为毒物,影响着全球数百万人的健康。虽然流行病学、体内和体外数据丰富,但臭氧毒性主要集中在单个细胞类型(如上皮II型、肺泡巨噬细胞)或与损伤相关的信号通路(如akt、谷胱甘肽)中。如果使用得当,大量和单细胞RNA测序技术有可能提供完整的,在某些情况下无偏倚的,在稳态和损伤肺中发生的分子事件的信息,甚至捕获邻近细胞的表型多样性。为此,本综述汇编了有关稳态和臭氧暴露肺细胞身份和激活的最新了解的信息。此外,它还讨论了多组学方法和其他用于预测细胞-细胞通信和解剖空间异质性的工具的价值和益处。
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来源期刊
Current opinion in toxicology
Current opinion in toxicology Toxicology, Biochemistry
CiteScore
8.50
自引率
0.00%
发文量
0
审稿时长
64 days
期刊最新文献
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