Intercropping and Co-Inoculation of Beneficial Microorganisms of Soils Improve Drought Tolerance in Barley and Alfalfa Plants

IF 2.4 3区 农林科学 Q1 AGRONOMY Gesunde Pflanzen Pub Date : 2023-11-17 DOI:10.1007/s10343-023-00949-7
Aiman Slimani, Khalid Oufdou, Abdelilah Meddich
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Abstract

Drought is one of the main devastating environmental factors limiting crops’ development and productivity. This study investigated the role of combining intercropping and co-inoculation of arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) to protect barley and alfalfa against drought damage. The experiment design consisted of four inoculation treatments: (1) non-inoculated plants (C), (2) plants inoculated with AMF consortium (AMF), (3) plants inoculated with the bacterial consortium (PGPR), and (4) plants co-inoculated with AMF + PGPR (AMF + PGPR), and two irrigation regimes: (i) well-watered, equivalent to 75% field capacity (FC), and (ii) drought, where watering was maintained at 35% FC. For each treatment (inoculated or not inoculated and stressed or not stressed), the plants of barley and alfalfa were monocropped and intercropped. Growth (shoots and roots dry weight), physiological (stomatal conductance and chlorophyll fluorescence), and biochemical (stress markers, osmolytes contents, and antioxidant enzyme activities) parameters were all measured. The results showed that applying intercropping and microbial inoculation AMF or/and PGPR enhanced the tolerance of plants to drought stress. The most pronounced effect was displayed by combining intercropping system and co-inoculation of AMF + PGPR, which improved shoot and root dry weight by 141 and 280% in barley and by 512 and 533% in alfalfa, respectively, compared to their respective uninoculated monocultures. Similarly, combining intercropping and co-inoculation with AMF + PGPR enhanced acid phosphatase, superoxide dismutase, and catalase activities by 125%, 161%, and 58% in barley and by 114%, 311%, and 112% in alfalfa, respectively, compared to their respective uninoculated monocultures. Furthermore, the thousand-seed weight was increased by 73% in barley intercropped and inoculated with AMF +PGPR. These findings revealed that intercropping barley and alfalfa and co-inoculation of AMF +PGPR may provide a sustainable approach to enhance drought tolerance, increase crop productivity, and promote food security.

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间作和共接种土壤有益微生物可提高大麦和苜蓿的耐旱性
干旱是制约作物生长发育和生产力的主要破坏性环境因素之一。研究了丛枝菌根真菌(AMF)与植物促生根瘤菌(PGPR)套作联合接种对大麦和苜蓿抗旱的保护作用。试验设计包括四种接种处理:(1)未接种植株(C), (2) AMF联合体接种植株(AMF),(3)细菌联合体接种植株(PGPR), (4) AMF + PGPR共接种植株(AMF + PGPR),以及两种灌溉方式:(i)水分充足,相当于75%的田间容量(FC), (ii)干旱,其中水分保持在35% FC。各处理(接种或不接种、胁迫或不胁迫)大麦和苜蓿采用单作和间作。测定生长(茎和根干重)、生理(气孔导度和叶绿素荧光)和生化(胁迫标志物、渗透物含量和抗氧化酶活性)参数。结果表明,间作和微生物接种AMF / /和PGPR提高了植株对干旱胁迫的耐受性。间作组合与AMF + PGPR共接种效果最显著,大麦和苜蓿的茎部和根干重分别比未接种的单株提高了141和280%,512和533%。同样,与单独接种相比,间作与AMF + PGPR共接种可使大麦的酸性磷酸酶、超氧化物歧化酶和过氧化氢酶活性分别提高125%、161%和58%,苜蓿的酸性磷酸酶、超氧化物歧化酶和过氧化氢酶活性分别提高114%、311%和112%。间作接种AMF +PGPR的大麦千粒重提高了73%。这些结果表明,大麦和苜蓿间作和AMF +PGPR共接种可能是提高作物耐旱性、提高作物生产力和促进粮食安全的可持续途径。
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来源期刊
Gesunde Pflanzen
Gesunde Pflanzen 农林科学-农艺学
CiteScore
3.50
自引率
25.80%
发文量
152
审稿时长
>12 weeks
期刊介绍: Gesunde Pflanzen publiziert praxisbezogene Beiträge zum Pflanzenschutz in Landwirtschaft, Forstwirtschaft, Gartenbau und öffentlichem Grün und seinen Bezügen zum Umwelt- und Verbraucherschutz sowie zu Rechtsfragen. Das Themenspektrum reicht von der Bestimmung der Schadorganismen über Maßnahmen und Verfahren zur Minderung des Befallsrisikos bis hin zur Entwicklung und Anwendung nicht-chemischer und chemischer Bekämpfungsstrategien und -verfahren, aber auch zu Fragen der Auswirkungen des Pflanzenschutzes auf die Umwelt, die Sicherung der Ernährung sowie zu allgemeinen Fragen wie Nutzen und Risiken und zur Entwicklung neuer Technologien. Jedes Heft enthält Originalbeiträge renommierter Wissenschaftler, aktuelle Informationen von Verbänden sowie aus der Industrie, Pressemitteilungen und Personalia. Damit bietet die Zeitschrift vor allem Behörden und Anwendern im Agrarsektor und Verbraucherschutz fundierte Praxisunterstützung auf wissenschaftlichem Niveau.
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