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Genetic Association Among Morpho-Physiological Attributes Against Heat Tolerance in Rice Genotypes 水稻基因型的形态-生理特征与耐热性之间的遗传关联
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-08-02 DOI: 10.1007/s10343-024-00998-6
Hafiz Ghulam Muhu-Din Ahmed, Noor Fatima, Yawen Zeng, Muddassir Hussain, Muhammad Moaaz Mushtaq, Ghulam Sabir Hussain, Muhammad Irfan Akram, Amjad Saeed, Adnan Noor Shah, Hayssam M. Ali, Mohamed M. A. Abdelhamid, Sezai Ercisli

Asia produces the majority of the world’s rice (Oryza sativa L.), one of the utmost vital fundamental food crops, with 90% of worldwide production. Because of increases in average temperature, climate change has a negative impact on rice yield. It is essential to develop such verities of rice that have potential to meet challenges of environmental changes like heat stress etc. This research was approved to investigate heat tolerance ability of rice genotypes on the basis of morpho-physiological attributes at the experimental field area of Islamia University of Bahawalpur. Using a randomized complete blocked design (RCBD) and two sets of plots (one in early sowing rice, other plot goes under late sowing rice condition) with five replications was applied to study 102 genotypes of rice under heat stress. The days to heading, days to maturity, no. of panicles, flag leaf area, grain weight per panicle, no. of grains per panicle, 1000 grain weight and grain yield per plant were positively correlated among themselves under both early sowing and late sowing conditions. Results revealed that, out of 14 principal components (PCs), the first five PCs were showed significant genetic variation under both conditions. The first five PCs displayed 71.1% and 68.9% total variance in the examined germplasm under early and late sowing locations respectively. Based on PCA, 05 heat-tolerant and five heat-susceptible genotypes were identified. There is a significant difference in all the studied rice morphological and physiological traits. Effect of high temperature (heat stress) was observed in both early (normal) and late sowing (heat stress) environments. As a result of these findings selection based on these qualities is not appropriate for heat tolerance. The best performing germplasm under heat stress can be a desirable genotype to generate high yield and used for future breeding programs and early sowing of rice genotypes recommended in this study will be effective for developing heat tolerant rice varieties.

水稻(Oryza sativa L.)是最重要的基本粮食作物之一,亚洲生产了世界上大部分的水稻,占全球产量的 90%。由于平均气温升高,气候变化对水稻产量产生了负面影响。因此,必须开发出有潜力应对热胁迫等环境变化挑战的水稻品种。这项研究的目的是在巴哈瓦尔布尔伊斯兰大学的试验田里,根据形态-生理特性调查水稻基因型的耐热能力。研究采用随机完全区组设计(RCBD)和两组小区(一组在早稻播种条件下,另一组在晚稻播种条件下)五次重复的方法,对 102 个水稻基因型进行了热胁迫下的研究。在早播和晚播条件下,水稻的打顶天数、成熟天数、圆锥花序数、旗叶面积、每圆锥花序粒重、每圆锥花序粒数、千粒重和单株谷物产量之间均呈正相关。结果显示,在 14 个主成分(PCs)中,前 5 个 PCs 在两种条件下均表现出显著的遗传变异。在早播和晚播条件下,前五个主成分分别显示了 71.1%和 68.9%的总方差。根据 PCA,确定了 05 个耐热基因型和 5 个感热基因型。所研究的所有水稻形态和生理性状均存在显著差异。在早播(正常)和晚播(热胁迫)环境中都观察到了高温(热胁迫)的影响。因此,根据这些性状进行选择并不适合耐热性。在热胁迫下表现最好的种质可以成为产生高产的理想基因型,并可用于未来的育种计划,而本研究推荐的早播水稻基因型将有效开发耐热水稻品种。
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引用次数: 0
Efficacy of Bioinsecticides, Synthetic Insecticides, Macro and Micronutrients for the Management of Onion Thrips, Thrips tabaci in Onion: a Field Trial 生物杀虫剂、合成杀虫剂、宏观和微观营养元素对防治洋葱蓟马的功效:田间试验
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-17 DOI: 10.1007/s10343-024-01001-y
El-Said Mohamed Hassan, Mohamed F. El-Sheikh, Fatma H. Hegazy, Fathi A. Ali, Ibrahim Ibrahim Mesbah

Onion thrips, Thrips tabaci Lind. (Thysanoptera: Thripidae) is a major pest of onion worldwide. It damages onion, Allium cepa L., both directly and indirectly, which leads to significant output losses. In 2022, research was conducted in a private onion field in the Delta region, Egypt, to evaluate the efficacy of bioinsecticides and synthetic insecticides and to evaluate the efficacy of macro- and micronutrient in determining treatment effects on onion thrips populations.

Injury caused by onion thrips is often not severe enough to warrant the application of numerous pesticides to crops in the Delta region of Egypt. Spinosad (Tracer 24% SC) and spinetoram (Radiant 12% SC) were used as bioinsecticides, and Pyridalyl (Pleo 50% EC) was used as a synthetic insecticide for managing onion thrips populations. The greatest decrease in infestation was achieved by the application of the chemical insecticide (Pyridalyl) Pleo 50% EC and the bioinsecticide (Spinosad) Tracer 24% SC. The greatest decrease in infestation was achieved by the application of the micronutrient boron.

洋葱蓟马(Thrips tabaci Lind.(Thysanoptera: Thripidae)是全世界洋葱的主要害虫。它直接或间接地危害洋葱(Allium cepa L.),导致严重的产量损失。2022 年,在埃及三角洲地区的一块私人洋葱田里开展了一项研究,以评估生物杀虫剂和合成杀虫剂的功效,并评估宏观和微观营养元素在确定洋葱蓟马种群处理效果方面的功效。在埃及三角洲地区,洋葱蓟马造成的伤害通常并不严重,不足以对作物施用大量杀虫剂。Spinosad(Tracer 24% SC)和 spinetoram(Radiant 12% SC)被用作生物杀虫剂,Pyridalyl(Pleo 50% EC)被用作合成杀虫剂,用于控制洋葱蓟马的数量。使用化学杀虫剂(哒螨灵)Pleo 50%EC和生物杀虫剂(斯皮诺沙得)Tracer 24% SC能最大程度地减少虫害。施用微量营养元素硼能最大程度地减少虫害。
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引用次数: 0
Influence of Plant Growth Promoting Rhizobacteria (PGPR) on the Growth and Yield of Sunflower (Helianthus annus L.) Under Salt Stress 盐胁迫下植物生长促进根瘤菌(PGPR)对向日葵(Helianthus annus L.)生长和产量的影响
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-09 DOI: 10.1007/s10343-024-01006-7
Shafi Ullah, Muhammad Ikram, Samina Sarfaraz, Ijaz ul Haq, Atika Khan, Zaryab Murad, Fazal Munsif

Phytoremediation techniques include phyto-de-salinization, phytodegradation, phytoextraction, phyto-filtering, and phyto-volatilation. The phytoremediation technique is considered a promising method to remediate saline soils. The study was conducted with two concentrations of saline soil (50 and 100 mM) with one control, followed by treatment of two isolates (Azospirillum brasilense and Pseudomonas fluorescence). The experiment was conducted in a randomized complete block design using two hybrids (Hysun-33 & Hysun-39). The inoculation of Azospirillum brasilense increased the total biomass production, flowering characteristics, and yield of sunflowers. The study parameter’s result showed that using plant growth-promoting rhizobacteria (PGPR) gives successful vegetative growth with good flowering characteristics and induced salt tolerance in sunflowers. So, bacterial isolate Azospirillum brasilense conformed to better vegetative growth and flowering than isolates of Pseudomonas fluorescence. There was an increase in antioxidants, e.g., catalase, peroxidase, ascorbate peroxidase, and proline content, and a decrease in hydrogen peroxide content of plants by inoculation of Azospirillum brasilense and Pseudomonas fluorescence. Decreased growth at higher salinity levels causes hyper-ionic and hyperosmotic stress and can lead to plant demise. From the results obtained, it could be recommended that Azospirillum brasilense be used to induce salt tolerance in sunflower (Helianthus annus L.) cv. “Hysun-33” under high salinity levels increases vegetative growth, flowering character, yield, and quality of sunflower.

植物修复技术包括植物脱盐、植物降解、植物萃取、植物过滤和植物挥发。植物修复技术被认为是一种很有前景的修复盐碱土壤的方法。研究使用了两种浓度的盐碱土(50 毫摩尔和 100 毫摩尔),其中一种为对照组,其次是两种分离菌(巴西绿氮菌和荧光假单胞菌)。实验采用随机完全区组设计,使用两种杂交种(Hysun-33 & Hysun-39)。接种 Azospirillum brasilense 增加了向日葵的总生物量产量、开花特性和产量。研究参数结果表明,使用植物生长促进根瘤菌(PGPR)能成功地促进向日葵的无性生长和良好的开花特性,并诱导向日葵的耐盐性。因此,细菌分离物 Azospirillum brasilense 比荧光假单胞菌分离物具有更好的无性生长和开花特性。接种 Azospirillum brasilense 和荧光假单胞菌后,植物的抗氧化剂,如过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和脯氨酸含量增加,过氧化氢含量减少。在盐度较高的情况下,生长量的减少会造成高离子和高渗透胁迫,并可能导致植物死亡。从所获得的结果来看,可以建议使用巴西鹅膏菌(Azospirillum brasilense)诱导向日葵(Helianthus annus L. )"Hysun-33 "变种的耐盐性。在高盐度条件下,"Hysun-33 "可提高向日葵的无性系生长、开花特性、产量和质量。
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引用次数: 0
Response of Yield, Antioxidant Enzymes Activities, and Fatty Acids in Peanut (Arachis hypogaea L.) to Bio-fertilizers and Amino Acids in Different Irrigation Regimes 不同灌溉制度下花生(Arachis hypogaea L.)的产量、抗氧化酶活性和脂肪酸对生物肥料和氨基酸的响应
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-05 DOI: 10.1007/s10343-024-00997-7
Soheila Najafalizadeh, Seyed Ali Mohammad Modarres-Sanavy, Marefat Mostafavi-Rad, Ali Mokhtassi-Bidgoli

Bio-fertilizers (BF) and amino acids (AA) improve plant growth by activating antioxidant enzymes and establishing a favorable metabolic environment to maintain physiological balance and drought stress mitigation. This experiment aimed to assess the influence of some BF and AA on peanuts (Arachis hypogaea L.) under drought stress. It was conducted during 2018–2019 as a split-split plot based on a randomized complete blocks design with three replications in the experimental field of the Agricultural and Natural Resources Research and Education Center of Guilan province, Rasht, Iran. Three irrigation regimes including irrigation after 75 (IR75), 100 (IR100), and 125 (IR125) mm evaporation from class A evaporation pan, four levels of BF including no application of bio-fertilizers as control (Z0), Arbuscular mycorrhiza (AM), Azospirillum brasilense (AB), Pseudomonas fluorescens (SF) and four levels of AA including no application of AA as control (Z0), Aminol-Forte (AF), Humi-Forte (HF) and Pro-Amin (PA) comprised experimental treatments as main plot, sub-plot and sub-sub plot, respectively. Peanut seed yield (SY) decreased under intensive drought stress. In 2018 and 2019, peanut SY increased by 43.34 and 38.08% in IR75 compared to control, under the interaction of AB×HF and SF×HF, respectively. The interaction of AB and HF increased antioxidant enzymes and improved SY under drought stress, which can be considered an innovation. Hence, the application of AB and HF could be recommended to enhance peanut SY under similar climatic conditions.

生物肥料(BF)和氨基酸(AA)通过激活抗氧化酶和建立有利的代谢环境来维持生理平衡和缓解干旱胁迫,从而改善植物生长。本实验旨在评估干旱胁迫下一些生物肥料和氨基酸对花生(Arachis hypogaea L.)的影响。该试验于2018-2019年期间在伊朗拉什特市吉兰省农业与自然资源研究和教育中心的试验田中进行,采用随机完全区组设计,三次重复,分小区进行。三种灌溉制度,包括从 A 级蒸发盘蒸发 75 毫米(IR75)、100 毫米(IR100)和 125 毫米(IR125)后灌溉,四种生物肥料水平,包括不施用生物肥料作为对照(Z0)、丛枝菌根(AM)、Azospirillum brasil、Azospirillum brasil、Azospirillum brasil、Azospirillum brasil、Azospirillum brasil)和 BF、Azospirillum brasilense (AB)、荧光假单胞菌 (SF) 和四级 AA(包括不施用 AA 作为对照 (Z0))、Aminol-Forte (AF)、Himi-Forte (HF) 和 Pro-Amin (PA))分别组成主小区、副小区和子小区的实验处理。在密集干旱胁迫下,花生种子产量(SY)下降。2018年和2019年,在AB×HF和SF×HF的交互作用下,IR75的花生SY与对照相比分别增加了43.34%和38.08%。AB和HF的交互作用提高了抗氧化酶,改善了干旱胁迫下的SY,可以说是一种创新。因此,建议在类似气候条件下应用 AB 和 HF 提高花生 SY。
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引用次数: 0
Emergence Pattern of Argemone mexicana, Brassica tournefortii, and Rapistrum rugosum in Eastern Australia 澳大利亚东部 Argemone mexicana、Brassica tournefortii 和 Rapistrum rugosum 的出现模式
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-04 DOI: 10.1007/s10343-024-01003-w
Gulshan Mahajan, Bhagirath Singh Chauhan

A study assessed the potential for using cumulative growing degree days (CGDD) to predict the weed emergence periodicity of three weed species: Argemone mexicana, Brassica tournefortii, and Rapistrum rugosum. Weed emergence was monitored regularly by placing 200 fresh seeds of each weed species on the soil surface. Weed emergence data was fit using a three-parameter sigmoidal Gompertz model. The CGDD required for 50% emergence of A. mexicana ranged from 3380 to 5302, depending upon the seasonal variation in temperature and rainfall. The majority of emergence appeared from March to June. The seeds of A. mexicana exhibited dormancy, as the majority of seeds germinated in the second season. The CGDD required for 50% emergence of B. tournefortii ranged from 824 to 2311, depending upon the seasonal variation in temperature and intensity of rainfall. Most cohorts of B. tournefortii appeared in the first season from February to June, indicating little dormancy in seeds. The CGDD required for 50% emergence of R. rugosum ranged from 2242 to 2699, depending upon weather parameters (temperature and rainfall). The main cohorts of R. rugosum appeared from February to June, and 60% of seeds germinated in the first season, while 40% germinated in the second season, indicating dormancy in seeds. The coefficients of determination for the model verification on the emergence pattern of three weeds were > 85%, suggesting that CGDD are good predictors for the emergence of these weeds. These results suggest that forecasting the emergence of three weed species on the basis of CGDD and rainfall patterns will help growers to make better weed management decisions.

一项研究评估了利用累积生长度日(CGDD)预测三种杂草出苗周期的潜力:Argemone mexicana、Brassica tournefortii 和 Rapistrum rugosum。通过在土壤表面放置 200 颗每种杂草的新鲜种子,定期监测杂草的出苗情况。杂草萌发数据采用三参数半正方形贡珀兹模型进行拟合。根据气温和降雨量的季节性变化,50% 的墨西哥豚草出苗所需的 CGDD 为 3380 至 5302。大部分萌发出现在 3 月至 6 月。A. mexicana 的种子有休眠现象,大多数种子在第二季萌发。根据温度和降雨强度的季节性变化,B. tournefortii 50%萌发所需的 CGDD 为 824 至 2311。大多数 B. tournefortii 在第一季(2 月至 6 月)萌发,表明种子几乎没有休眠。R. rugosum 50%萌发所需的 CGDD 为 2242 至 2699,取决于天气参数(温度和降雨量)。R. rugosum 的主要出苗期为 2 月至 6 月,60% 的种子在第一季发芽,40% 的种子在第二季发芽,这表明种子存在休眠。对三种杂草出苗模式的模型验证的决定系数为 85%,表明 CGDD 可以很好地预测这些杂草的出苗。这些结果表明,根据 CGDD 和降雨模式预测三种杂草的出苗情况将有助于种植者做出更好的杂草管理决策。
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引用次数: 0
Exogenous Potassium Silicate Improves Drought Tolerance in Cotton Genotypes by Modulating Growth, Gas Exchange and Antioxidant Metabolism 外源硅酸钾通过调节生长、气体交换和抗氧化代谢提高棉花基因型的抗旱能力
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-01 DOI: 10.1007/s10343-024-01005-8
Muhammad Nazim, Xiangyi Li, Akash Tariq, Khurram Shahzad, Murad Muhammad, Zhang Dawei, Fanjiang Zeng

Cotton is a highly sensitive crop to drought stress. Consequently, it is crucial to devise strategies that optimize crop production in conditions of limited water availability. While potassium silicate has demonstrated effectiveness in mitigating drought stress in various crops, its specific impact on different cotton cultivars under drought conditions remains not fully clarified. This research aimed to assess the efficacy of six potassium silicate levels (0, 100, 200, 300, 400 and 500 mg L−1) on four cotton genotypes (Zong main-113, Xin Nong-525, Xin lu Zhong-55, and Xin lu Zhong-66) under two field capacity levels (80% and 50% FC) in a sand culture. Foliar applied potassium silicate significantly improved photosynthetic efficiency, shoot biomass, root biomass, and leaf area under water stress (50% FC). The most substantial reduction in H2O2, malondialdehyde levels, and electrolyte leakage was recorded with potassium silicate applied at a rate of 400 mg L−1. This concentration effectively mitigated reactive oxygen species accumulation, safeguarding plants against oxidative damage at 50% FC. Furthermore, potassium silicate contributed to maintaining water status, resulting in increased leaf water content and elevated water-soluble proteins in cotton plants. The order of drought resistance (50% FC) with the application of potassium silicate at 400 mg L−1 was Zong Mian-113, Xin Nong-525, Xin lu Zhong-55, and Xin lu Zhong-66. The findings could help in selection of drought resistance cultivars of cotton in water limited conditions.

棉花是一种对干旱胁迫高度敏感的作物。因此,在水资源有限的条件下,制定优化作物生产的策略至关重要。虽然硅酸钾在缓解各种作物的干旱胁迫方面已被证明有效,但它在干旱条件下对不同棉花品种的具体影响仍未完全阐明。本研究旨在评估在两种田间能力水平(80% FC 和 50% FC)下,在沙培条件下六种硅酸钾水平(0、100、200、300、400 和 500 mg L-1)对四种棉花基因型(宗主-113、新农-525、新鲁中-55 和新鲁中-66)的功效。在水分胁迫(50% FC)条件下,叶面喷施硅酸钾能显著提高光合效率、增加芽生物量、根生物量和叶面积。硅酸钾施用量为 400 mg L-1 时,H2O2、丙二醛水平和电解质渗漏的降低幅度最大。这一浓度能有效缓解活性氧的积累,保护植物在 50% FC 条件下免受氧化损伤。此外,硅酸钾还有助于维持水分状态,使棉花植株叶片含水量增加,水溶性蛋白质升高。施用 400 mg L-1 的硅酸钾后,棉株的抗旱性(50% FC)依次为宗棉-113、新农-525、新鲁中-55 和新鲁中-66。这些发现有助于在限水条件下选育抗旱棉花品种。
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引用次数: 0
Effects of Newly Isolated Rhizobacteria on the Physiological Characteristics and Nutrient Uptake of Watermelon Plants Grafted onto Different Rootstocks Under Water Stress 新分离的根瘤菌对水分胁迫下不同砧木上嫁接西瓜植株的生理特征和养分吸收的影响
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-27 DOI: 10.1007/s10343-024-01004-9
Duran Yavuz, Fatma Gökmen Yılmaz, Musa Seymen, Ayşegül Korkmaz, Kubilay Kurtuluş Baştaş

Currently, many techniques to alleviate the negative effects of water stress, specifically drought, on plants are frequently the subject of research. In this study, the effects of two different plant growth-promoting rhizobacterial (PGPR) species isolated from arid and semiarid areas whose activities under water stress were determined in a preliminary study on several physiological properties and nutrient uptake of watermelons grafted onto different rootstocks were investigated under deficit irrigation. In this study, the performance of two PGPRs (B1: Paenarthrobacter aurescens and B2: Pseudarthrobacter polychromogenes) inoculated into ungrafted watermelon (R0 (Citrullus lanatus (Thumb.) Matsum and Nakai cv. Crimson Tide, CT)) and grafted watermelons (R1: CT grafted onto citron watermelon rootstock (Citrullus lanatus var. Citroides) and R2: CT grafted onto the hybrid rootstock TZ-148 (Cucurbita maxima Duchesne×Cucurbita moschata Duchesne)) were studied under different irrigation levels. Severe water stress negatively affected the physiological characteristics, such as stomatal conductance (gs) and photosynthesis efficiency (QPSII), of watermelon plants. Moreover, the contents of leaf mineral nutrients such as N, P, and K decreased significantly with increasing water stress. On the other hand, rootstocks improved the performance of sweet watermelon in terms of macronutrients such as N, K, and Mg and micronutrients such as Fe and Cu. Moreover, the drought-tolerant rootstocks (R1 and R2) used in this study protected watermelon plants against the negative effects of water stress by reducing gs compared to that of ungrafted plants. Moreover, although rhizobacteria did not have a significant effect on the gs, QPSII, or leaf water potential (LWP) of watermelon, they enhanced the uptake of minerals such as macro- and micronutrients from the soil by plants. Under full irrigation and particularly deficit irrigation, P. polychromogenes (B2) increased the contents of macronutrients such as Mg and K, while P. aurescens (B1) increased the contents of micronutrients such as Fe, Cu, Mn, and B in watermelons. Our results revealed that these two rhizobacterial species, which were isolated from arid and semiarid areas, contribute to the nutrient uptake of watermelon plants grown under water stress.

目前,许多缓解水胁迫(特别是干旱)对植物负面影响的技术经常成为研究的主题。在本研究中,通过初步研究确定了从干旱和半干旱地区分离出来的两种不同的植物生长促进根瘤菌(PGPR)在缺水灌溉条件下对嫁接到不同砧木上的西瓜的几种生理特性和养分吸收的影响。在这项研究中,将两种 PGPRs(B1:Paenarthrobacter aurescens 和 B2:Pseudarthrobacter polychromogenes)接种到未嫁接的西瓜(R0(Citrullus lanatus (Thumb.) Matsum and Nakai cv.Crimson Tide,CT)和嫁接西瓜(R1:CT 嫁接到柠檬西瓜砧木(Citrullus lanatus var:在不同灌溉水平下,对嫁接到杂交砧木 TZ-148 (Cucurbita maxima Duchesne × Cucurbita moschata Duchesne)上的 CT 进行了研究。严重的水分胁迫对西瓜植株的气孔导度(gs)和光合作用效率(QPSII)等生理特性产生了负面影响。此外,随着水分胁迫的加剧,叶片矿质养分(如氮、磷和钾)的含量也显著下降。另一方面,砧木提高了甜西瓜在氮、钾、镁等宏量营养元素和铁、铜等微量营养元素方面的表现。此外,与未嫁接的植株相比,本研究中使用的耐旱砧木(R1 和 R2)可降低 gs,从而保护西瓜植株免受水分胁迫的负面影响。此外,虽然根瘤菌对西瓜的 gs、QPSII 或叶片水势(LWP)没有显著影响,但它们提高了植物从土壤中吸收矿物质(如大分子和微量元素)的能力。在充分灌溉尤其是亏缺灌溉条件下,多色根瘤菌(B2)增加了西瓜中镁和钾等宏量营养元素的含量,而金色根瘤菌(B1)则增加了西瓜中铁、铜、锰和硼等微量营养元素的含量。我们的研究结果表明,这两种从干旱和半干旱地区分离出来的根瘤菌有助于西瓜植株在水分胁迫下吸收养分。
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引用次数: 0
Restricted Irrigation Regimes and Rapeseed High-Yielding Genotypes Can Be Applied to Cope With the Water Shortage Crisis and More Stable Oil Production 限制灌溉制度和油菜籽高产基因型可用于应对缺水危机和更稳定的石油生产
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-26 DOI: 10.1007/s10343-024-00995-9
Saber Saif Amiri, Mehrdad Yarnia, Bahram Mirshekari, Farhad Farahvash, Varahram Rashidi

To investigate the quantitative and qualitative yields of different rapeseed genotypes under optimal water supply and restricted irrigation regimes, a split plot experiment was arranged in a randomized complete blocks design with three replicates in the 2018–19 and 2019–20 growing seasons. The Main plots included irrigation regimes at three levels of optimal water supply regime, withholding irrigation from the beginning of flowering, and withholding irrigation from the beginning of silique setting. The sub-plots contained 11 Iranian rapeseed genotypes (Dalgan, Zafar, Sarigol, RGS003, Hyola4815, Hyola401, Zabol10, Jerom, Jerry, Julius, and Jacomo). The highest rapeseed seed yield (3463 kg ha−1), oil content (43.09%), and oil yield (1492 kg ha−1) were obtained from the optimal water supply regime. Drought stress (restricted irrigation from silique setting and flowering stages) decreased seed yield (28 and 49%), oil content (4 and 7%), and oil yield (31 and 52%). Fatty acids compositions of rapeseed genotypes differently responded to drought stress conditions. Restricted irrigation regimes enhanced the linolenic acid, erucic acid, and glucosinolate contents, while the palmitic acid, oleic acid, and linoleic acid content were reduced when the rapeseed plants were subjected to drought stress. Overall, our findings suggest that the Sarigol genotype due to acceptable seed and oil yield as well as high-quality oil under three studied irrigation regimes can be recommended for cultivation in arid and semi-arid regions like Iran.

为研究不同油菜基因型在最佳供水和限制灌溉制度下的定量和定性产量,在2018-19和2019-20生长季,采用随机完全区组设计,安排了三个重复的分小区试验。主小区包括三个层次的灌溉制度:最佳供水制度、从开花期开始停止灌溉和从坐果期开始停止灌溉。子小区包括 11 个伊朗油菜基因型(Dalgan、Zafar、Sarigol、RGS003、Hyola4815、Hyola401、Zabol10、Jerom、Jerry、Julius 和 Jacomo)。在最佳供水条件下,油菜籽产量(3463 千克/公顷)、含油量(43.09%)和出油率(1492 千克/公顷)最高。干旱胁迫(在油菜抽薹期和开花期限制灌溉)降低了油菜籽产量(28% 和 49%)、含油量(4% 和 7%)和出油率(31% 和 52%)。油菜籽基因型的脂肪酸组成对干旱胁迫条件的反应不同。当油菜植株受到干旱胁迫时,限制灌溉制度会提高亚麻酸、芥酸和葡萄糖苷酸的含量,而棕榈酸、油酸和亚油酸的含量则会降低。总之,我们的研究结果表明,在所研究的三种灌溉制度下,Sarigol 基因型具有可接受的种子和油产量以及高品质的油,可推荐在伊朗等干旱和半干旱地区种植。
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引用次数: 0
Biocontrol of Bacterial Wilt Biotic Stress in Tomato Plants by Successful Host Root Colonization and Inducing Host Resistance 通过成功的寄主根定植和诱导寄主抗性对番茄植物细菌枯萎病生物胁迫进行生物控制
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-25 DOI: 10.1007/s10343-024-01002-x
Xiang Li, Saleh Alfarraj, Mohammad Javed Ansari

Plant treatments with biocontrol agents to deal with biotic stress are widely reported, but the information regarding detailed action mechanisms of biocontrol and host response is rarely reported. This study investigated a biocontrol bacterial agent, Bacillus cereus, to manage tomato bacterial wilt (BW) disease. The in vitro antibacterial potential of B. cereus was assessed, followed by the ability of B. cereus to colonize tomato roots and induce host resistance. Additionally, we tested the application of B. cereus for managing tomato BW disease. In vitro investigations revealed the volatile mediated antibacterial activity of B. cereus, indicating that B. cereus produces antibacterial volatiles against R. solanacearum. The effectiveness of B. cereus in colonizing tomato roots was evaluated through its transgenic GFP-tagged strains and confirmed through qPCR analysis. It was found that the biocontrol bacterium successfully colonized the host root. The B. cereus concentration reached 9.37 × 107 at 48 h. The tomato plants under bacterial wilt stress, when treated with B. cereus, showed upregulation of genes linked to the plant defense system. The application of B. cereus to soil infested with R. solanacearum and planted with tomato plants reduced the pathogen population in the soil, resulting in a reduction in disease severity and improved plant growth. This study suggests the biocontrol potential of B. cereus to manage bacterial wilt disease.

用生物控制剂处理植物以应对生物胁迫的报道很多,但有关生物控制剂的详细作用机制和宿主反应的信息却很少报道。本研究调查了一种生物防治菌剂--蜡样芽孢杆菌(Bacillus cereus),用于控制番茄细菌性枯萎病(BW)。首先评估了蜡样芽孢杆菌的体外抗菌潜力,然后评估了蜡样芽孢杆菌在番茄根部定殖和诱导宿主抗性的能力。此外,我们还测试了应用 B. cereus 控制番茄枯萎病的情况。体外研究显示,蜡样芽孢杆菌具有挥发性介导的抗菌活性,表明蜡样芽孢杆菌能产生抗菌挥发物来对抗茄黄萎病菌。通过转基因 GFP 标记菌株评估了蜡样芽孢杆菌在番茄根部定殖的有效性,并通过 qPCR 分析进行了确认。结果发现,该生物防治菌成功地在寄主根部定殖。48 小时后,B. cereus 的浓度达到 9.37 × 107。细菌枯萎病胁迫下的番茄植株经 B. cereus 处理后,与植物防御系统相关的基因出现上调。在种植番茄的土壤中施用 B. cereus,可减少土壤中的病原体数量,从而降低病害严重程度,改善植物生长。这项研究表明,B. cereus 具有管理细菌枯萎病的生物防治潜力。
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引用次数: 0
Botanical Insecticide Based on Annona mucosa Agro-industrial Waste as an Alternative to Manage Lepidopteran Soybean Defoliators 基于农用工业废弃物的植物杀虫剂,作为管理鳞翅目大豆落叶害虫的替代品
IF 3.1 3区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-20 DOI: 10.1007/s10343-024-00996-8
Camila Moreira de Souza, Thaís Lohaine Braga dos Santos, Alisson da Silva Santana, José Djair Vendramim, Edson Luiz Lopes Baldin, Leandro do Prado Ribeiro

Fruit processing waste from Annona mucosa Jacq. (Annonaceae) is an interesting source of biomass for development of ecofriendly botanical insecticides. Here, we evaluated the lethal and sublethal effects of the ethanolic extract prepared from seeds of A. mucosa (ESAM; major component = acetogenin bis-tetrahydrofuran rolliniastatin-1) against Chrysodeixis includens (Walker) and Anticarsia gemmatalis Hübner, two major defoliators of soybean crops. In the initial trial, ESAM showed activity comparable or superior to our positive control (Azamax® 1.2 EC, at recommended rate). The larvae of C. includes survived at higher levels than A. gemmatalis when exposed to ESAM. To cause mortality of 50 and 90% of the population of A. gemmatalis, 66.91 and 288.36 mg kg−1 was needed, respectively, whereas, for C. includens, 452.38 and 885.70 mg kg−1 were required, respectively. In addition, 27.23 and 51.01 h were needed to cause 50% of mortality against A. gemmatalis and C. includens, respectively. Under semi-field conditions (greenhouse), the aqueous emulsion of ESAM (pre-commercial formulation) and Azamax® 1.2 EC caused larval mortality above 95% for both pest species exposed to treated soybean plants. These results show that A. mucosa processing waste can be a source of promising botanical insecticides for the management of C. includens and A. gemmatalis, which are the main economically lepidopteran defoliators of soybean crops.

粘木茴香(Annona mucosa Jacq.,茴香科)的果实加工废料是开发生态友好型植物杀虫剂的一个有趣的生物质来源。在此,我们评估了从粘花艳红种子中提取的乙醇提取物(ESAM;主要成分=乙酰甙元双四氢呋喃roliniastatin-1)对大豆作物的两种主要落叶害虫 Chrysodeixis includens (Walker) 和 Anticarsia gemmatalis Hübner 的致死和亚致死作用。在最初的试验中,ESAM 的活性与我们的阳性对照(Azamax® 1.2 EC,推荐用量)相当或更优。当暴露于 ESAM 时,C. includes 的幼虫存活率高于 A. gemmatalis。要导致 50% 和 90% 的 A. gemmatalis 群体死亡,分别需要 66.91 和 288.36 毫克/千克,而 C. includeens 则分别需要 452.38 和 885.70 毫克/千克。此外,对 A. gemmatalis 和 C. includens 造成 50%的死亡率分别需要 27.23 和 51.01 小时。在半田间条件下(温室),ESAM(商业化前制剂)和 Azamax® 1.2 EC 的水乳剂对暴露于处理过的大豆植株上的两种害虫的幼虫死亡率均超过 95%。这些结果表明,粘虫加工废料可作为一种有前景的植物杀虫剂来源,用于防治大豆作物的主要鳞翅目落叶害虫 C. includens 和 A. gemmatalis。
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