Methoprene-tolerant and Krüppel-homologue 1 are involved in the fecundity improvement of Diaphorina citri induced by Cordyceps fumosorosea

IF 5.6 1区农林科学 Q1 ENTOMOLOGY Entomologia Generalis Pub Date : 2023-12-11 DOI:10.1127/entomologia/2023/2101

Shujie Wu, Jiayun Li, Shijian Tan, Jielan He, Desen Wang, Yijing Cen, Yurong He, Xiaoge Nian

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Abstract

Bacterial or virus can increase fecundity and other fitness-related traits of insect hosts for their own advantage, however reports on fungal induction of such effects are scarce. In a previous study, we found that low dose fungal treatment (Cordyceps fumosorosea) improved fecundity of Asian citrus psyllid (ACP), however underlying molecular mechanism(s) of such improvement remained unclear. Here, exposure of five-instar nymphs to low lethal concentration of C. fumosorosea (LC20) significantly lowered the adult emergence rate, shortened female adult longevity, accelerated the ovarian development, and improved the fecundity compared to untreated individuals. Consistently, exposure of ACP to LC20 C. fumosorosea significantly increased the juvenile hormone (JH) titer in the ovaries as well as the expression levels of JH receptor, methoprene-tolerant (DcMet) and downstream key transcription factor Krüppel homolog 1 (DcKr-h1). DcMet and DcKr-h1 knockdown markedly blocked ovarian development, reduced female fecundity and extended female longevity in females treated by LC20 C. fumosorosea. Moreover, JH analogue (methoprene) application partially rescued reproductive defects induced by DcMet and DcKr-h1 depletion. These results provided insights into the mechanisms underlying the effects of low concentrations of fungi on insect pests, and had applied implications for the control of ACP.

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耐甲氧苄啶和 Krüppel 同源体 1 参与了冬虫夏草诱导的枸杞多糖的繁殖力提高过程

细菌或病毒可提高昆虫宿主的繁殖力和其他与体质相关的性状，以获得自身的优势，但有关真菌诱导此类效应的报道却很少。在之前的一项研究中，我们发现低剂量真菌处理（冬虫夏草）可提高亚洲柑橘蚜（ACP）的繁殖力，但这种提高的潜在分子机制仍不清楚。与未处理的个体相比，将五龄若虫暴露于低致死浓度（LC20）的烟曲霉中可显著降低成虫出现率、缩短雌成虫寿命、加速卵巢发育并提高繁殖力。同样，将 ACP 暴露于 LC20 C. fumosorosea 能显著提高卵巢中幼年激素（JH）的滴度以及 JH 受体、耐甲氧丙芘（DcMet）和下游关键转录因子 Krüppel homolog 1（DcKr-h1）的表达水平。DcMet 和 DcKr-h1 的敲除明显阻碍了 LC20 C. fumosorosea 处理的雌虫的卵巢发育、降低了雌虫的繁殖力并延长了雌虫的寿命。此外，施用 JH 类似物（甲氧苄啶）可部分缓解 DcMet 和 DcKr-h1 缺失引起的生殖缺陷。这些结果有助于深入了解低浓度真菌对害虫的影响机制，并对防治 ACP 具有应用意义。

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来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.