Plasma lipidomics reveal systemic changes persistent throughout early life following a childhood burn injury

IF 6.3 1区 医学 Q1 DERMATOLOGY Burns & Trauma Pub Date : 2023-12-07 DOI:10.1093/burnst/tkad044
Eva Kierath, Monique Ryan, Elaine Holmes, Jeremy K Nicholson, Mark W Fear, Fiona M Wood, Luke Whiley, Nicola Gray
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Abstract

Background Non-severe paediatric burns can result in poor long-term health outcomes. This occurs even in cases with good acute burn-related outcomes, including minimal scarring. The mechanisms that underpin the transition from non-severe burn to sustained negative long-term health impacts are currently unknown. However, sustained metabolic and immune changes have been observed in paediatric burn studies, suggesting these changes may be important. The plasma lipidome consists of a rich pool of bioactive metabolites that play critical roles in systemic processes including molecular signalling and inflammation. We hypothesised that changes in the plasma lipidome may reflect underlying changes in health status and be linked to long-term health after burn trauma. Methods This study analysed the lipidome in children who had previously experienced a non-severe burn, compared to non-injured controls. Thirty-three participants were recruited between the ages of 5 and 8 years who had experienced a non-severe burn between the ages of 1 and 3 years. Plasma samples were also collected from a non-injured, healthy, age and gender matched control group (n = 21). Plasma lipids were measured using reversed-phase liquid chromatographymass spectrometery (LC-MS). Results In total 838 reproducible lipid species from 19 sub-classes passed quality control procedures and progressed to statistical analysis. Analysis of individual lipid metabolites showed significantly higher concentrations of lysophosphatidylethanolamines and phosphatidylethanolamines, and significantly lower concentrations in myristic, palmitic and palmitoleic acids in the plasma of those who had experienced burn injury compared to controls. Conclusion Long-term changes in the lipid profile may give insight into the mechanisms underlying poor long-term health subsequent to non-severe burn injury. Further work to investigate the relationship between long-term pathology and lipidomic changes may lead to a better understanding of the causes of secondary morbidity post-burn and to clinical intervention to reduce the long-term health burden of burn trauma.
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血浆脂质组学揭示了儿童烧伤后整个生命早期持续存在的系统性变化
背景非重度小儿烧伤可能导致不良的长期健康后果。即使在急性烧伤相关结果良好(包括瘢痕最小)的病例中也会出现这种情况。目前尚不清楚从非严重烧伤转变为持续的长期负面健康影响的机制。不过,在儿科烧伤研究中观察到了持续的新陈代谢和免疫变化,这表明这些变化可能很重要。血浆脂质体由丰富的生物活性代谢物组成,在包括分子信号传导和炎症在内的全身过程中发挥着关键作用。我们假设血浆脂质体的变化可能反映了健康状况的潜在变化,并与烧伤后的长期健康状况有关。方法 本研究分析了曾经历过非严重烧伤的儿童与未受伤的对照组儿童的血脂组。研究人员招募了 33 名年龄在 5 到 8 岁之间、在 1 到 3 岁之间经历过非严重烧伤的儿童。此外,还从未受损伤、健康、年龄和性别匹配的对照组(21 人)中采集了血浆样本。血浆脂质采用反相液相色谱-质谱法(LC-MS)进行测量。结果 共有 19 个亚类的 838 种可重复脂质通过了质量控制程序并进入统计分析。对单个脂质代谢物的分析表明,与对照组相比,烧伤患者血浆中溶血磷脂酰乙醇胺和磷脂酰乙醇胺的浓度明显较高,肉豆蔻酸、棕榈酸和棕榈油酸的浓度明显较低。结论 脂质谱的长期变化可能有助于了解非严重烧伤后长期健康状况不佳的机制。进一步研究长期病理变化与脂质组变化之间的关系,可能有助于更好地了解烧伤后继发性发病的原因,并有助于采取临床干预措施,减轻烧伤创伤对健康造成的长期负担。
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来源期刊
Burns & Trauma
Burns & Trauma 医学-皮肤病学
CiteScore
8.40
自引率
9.40%
发文量
186
审稿时长
6 weeks
期刊介绍: The first open access journal in the field of burns and trauma injury in the Asia-Pacific region, Burns & Trauma publishes the latest developments in basic, clinical and translational research in the field. With a special focus on prevention, clinical treatment and basic research, the journal welcomes submissions in various aspects of biomaterials, tissue engineering, stem cells, critical care, immunobiology, skin transplantation, and the prevention and regeneration of burns and trauma injuries. With an expert Editorial Board and a team of dedicated scientific editors, the journal enjoys a large readership and is supported by Southwest Hospital, which covers authors'' article processing charges.
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