Impact of structurally diverse polysaccharides on colonic mucin O-glycosylation and gut microbiota

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY npj Biofilms and Microbiomes Pub Date : 2023-12-11 DOI:10.1038/s41522-023-00468-3

Tong Zhao, Yue Zhang, Linhua Nan, Qing Zhu, Shukai Wang, Yutao Xie, Xinling Dong, Cui Cao, Xiaoliang Lin, Yu Lu, Yuxia Liu, Linjuan Huang, Guiping Gong, Zhongfu Wang

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Abstract

Understanding how dietary polysaccharides affect mucin O-glycosylation and gut microbiota could provide various nutrition-based treatments. Here, the O-glycan profile of the colonic mucosa and gut microbiome were investigated in C57BL/6J mice fed six structurally diverse dietary polysaccharides and a mixture of six fibers. Dietary polysaccharides increased total O-glycans, mainly by stimulating neutral glycans. Highly branched arabinogalactan promoted terminally fucosylated core 1 O-glycans; whereas linear polysaccharides, including pectin, konjac glucomannan, inulin, and the fiber mixture, favored terminally di-fucosylated O-glycans. The last three polysaccharides also lowered the level of sulfated O-glycans and sialylated mono-fucosylated O-glycans. Varied monosaccharide composition in mixed polysaccharides had a synergistic beneficial effect, boosting fucosylated neutral glycans, decreasing acidic glycans, and stimulating microbial richness and diversity. Dietary polysaccharides containing arabinose and sulfate groups enhanced the relative abundances of Akkermansia and Muribaculaceae, respectively. The present comparison reveals the relationship between dietary polysaccharide structure, mucin O-glycan composition, and intestinal microorganisms.

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结构多样的多糖对结肠粘蛋白 O-糖基化和肠道微生物群的影响

了解膳食多糖如何影响粘蛋白 O-糖基化和肠道微生物群，可以提供各种基于营养的治疗方法。在此，研究人员对喂食六种结构不同的膳食多糖和六种纤维混合物的 C57BL/6J 小鼠的结肠粘膜和肠道微生物群的 O 型糖谱进行了研究。膳食多糖主要通过刺激中性聚糖来增加 O 型聚糖总量。高支链阿拉伯半乳聚糖能促进末端岩藻糖基化的核心 1 O-聚糖；而包括果胶、魔芋葡甘露聚糖、菊粉和纤维混合物在内的线性多糖则有利于末端二岩藻糖基化的 O-聚糖。后三种多糖还降低了硫酸化 O 型聚糖和苷元化单褐藻糖基化 O 型聚糖的水平。混合多糖中不同的单糖成分具有协同增效作用，可提高岩藻糖基化中性聚糖的含量，降低酸性聚糖的含量，并刺激微生物的丰富性和多样性。含有阿拉伯糖和硫酸基团的膳食多糖分别提高了 Akkermansia 和 Muribaculaceae 的相对丰度。本比较揭示了膳食多糖结构、粘蛋白 O 型糖组成和肠道微生物之间的关系。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.