Esra Aksoy, Nilgün Güler, Ibrahim Sözdutmaz, Serkan Kökkaya, Engin Berber, Ayşe Gençay Göksu
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引用次数: 0
Abstract
Bovine rotaviruses (BRVs) are significant causative agents of severe diarrhea in newborn calves, resulting in substantial economic losses in the livestock industry. Inhibition of bovine rotavirus using extracts prepared from a Cucurbitaceae plant, which contains trypsin protease inhibitors, might offer a potential anti-rotaviral effect in vitro. Ecballium elaterium (E. elaterium) belongs to the Cucurbitaceae family, indigenous to the Mediterranean, contains E. elaterium trypsin isoinhibitors (EETIso), and has been used in traditional medicine. This study aimed to evaluate the in vitro efficacy of E. elaterium extract against bovine rotavirus infections. Ethanol extracts were prepared from E. elaterium seeds and fruit juice, and their non-toxic concentrations were determined using MA-104 cells. The cells were infected with bovine rotavirus in the presence of E. elaterium extract. The results demonstrated a significant decrease in the rotavirus titer in vitro upon treatment with the E. elaterium extract, suggesting its potential as a therapeutic agent against bovine rotavirus-induced diarrhea in calves. The utilization of E. elaterium extract may contribute to reduced calf mortality, lower medication costs, and improved economic value in cattle farming.
牛轮状病毒(brv)是新生牛犊严重腹泻的重要病原体,给畜牧业造成巨大的经济损失。从葫芦科植物中提取的含有胰蛋白酶抑制剂的提取物抑制牛轮状病毒,可能在体外提供潜在的抗轮状病毒作用。elballium elaterium (E. elaterium)属于葫芦科,原产于地中海,含有elballium elaterium胰蛋白酶同工抑制剂(etiso),已被用于传统医学。本研究旨在评价白莲叶提取物对牛轮状病毒感染的体外抑制作用。采用MA-104细胞法对莲蓬种子和果汁的乙醇提取物进行无毒浓度测定。在牛乳提取物存在的情况下,用牛轮状病毒感染细胞。结果表明,经牛乳提取物处理后,体外轮状病毒滴度显著降低,表明其有潜力作为治疗小牛轮状病毒引起的腹泻的药物。利用牛乳提取物可以降低小牛死亡率,降低药物成本,提高养牛业的经济价值。
期刊介绍:
Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.