Some biochemical characteristics of the hairy roots of Pisum sativum L. mutants

Q3 Agricultural and Biological Sciences Ecological genetics Pub Date : 2023-12-04 DOI:10.17816/ecogen568310
O. Timina, O. Timin, Anna Stepanova
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Abstract

Two high-protein root cultures of vegetable pea mutants were received [1]. In continuation a PCR analysis of the obtained root cultures genes was carried out according [2] and the amino acid composition of the cultures protein was clarified in a dry product on the AAA 339TM device [3]. Obtained results confirmed the absence of rhizobia contamination of the cultures, which grow steadily on a hormone-free media for 5 years. PCR analysis revealed that fourrolgenesA,B,C,Dwere inserted into the genome of the root culture with genotypeafaftltl, and two —rol Candrol D— in the genome of the root culture with genotypetltl. The protein composition of the obtained cultures was represented by essential and non-essential amino acids and some others. In four inserts culture, the content of essential, ketogenic, proteinogenic and sulfur-containing amino acids prevailed by 1.5–2 times. Two inserts culture has twice as much aspartic acid and proline. Both cultures lacked tryptophan. The number of inserts determines the amino acid composition most likely.
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豌豆突变体毛根的一些生化特征
获得了两个蔬菜豌豆突变体的高蛋白根培养物[1]。接着,根据[2]对获得的根培养基因进行PCR分析,并在AAA 339TM装置上用干燥剂澄清培养蛋白的氨基酸组成[3]。获得的结果证实了在无激素培养基上稳定生长5年的培养物没有根瘤菌污染。PCR分析显示,基因型为afaftll的根培养物基因组中插入了4个基因a、B、C、D,基因型为afaftll的根培养物基因组中插入了2个基因Candrol D -。所获得的培养物的蛋白质组成由必需氨基酸和非必需氨基酸以及其他一些氨基酸代表。在4个插入培养中,必需氨基酸、生酮氨基酸、蛋白质氨基酸和含硫氨基酸的含量占主导地位的1.5-2倍。两个插入培养有两倍的天冬氨酸和脯氨酸。两种培养都缺乏色氨酸。插入的数量最有可能决定氨基酸组成。
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来源期刊
Ecological genetics
Ecological genetics Environmental Science-Ecology
CiteScore
0.90
自引率
0.00%
发文量
22
期刊介绍: The journal Ecological genetics is an international journal which accepts for consideration original manuscripts that reflect the results of field and experimental studies, and fundamental research of broad conceptual and/or comparative context corresponding to the profile of the Journal. Once a year, the editorial Board reviews and, if necessary, corrects the rules for authors and the journal rubrics.
期刊最新文献
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