OsbZIP53 Negatively Regulates Immunity Response by Involving in Reactive Oxygen Species and Salicylic Acid Metabolism in Rice

IF 5.6 2区农林科学 Q1 AGRONOMY Rice Science Pub Date : 2024-03-01 DOI:10.1016/j.rsci.2023.12.002

Wu Lijuan , Han Cong , Wang Huimei , He Yuchang , Lin Hai , Wang Lei , Chen Chen , E. Zhiguo

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引用次数: 0

Abstract

The basic region/leucine zipper (bZIP) transcription factors play important roles in plant development and responses to abiotic and biotic stresses. OsbZIP53 regulates resistance to Magnaporthe oryzae in rice by analyzing APIP5-RNAi transgenic plants. To further investigate the biological functions of OsbZIP53, we generated osbzip53 mutants using CRISPR/Cas9 editing and also constructed OsbZIP53 over-expression transgenic plants. Comprehensive analysis of phenotypical, physiological, and transcriptional data showed that knocking-out OsbZIP53 not only improved disease resistance by inducing a hypersensitivity response in plants, but also regulated the immune response through the salicylic acid pathway. Specifically, disrupting OsbZIP53 increased H₂O₂ accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs (Osrboh genes) and weakened H₂O₂ degradation by directly targeting OsMYBS1. In addition, the growth of osbzip53 mutants was seriously impaired, while OsbZIP53 over-expression lines displayed a similar phenotype to the wild type, suggesting that OsbZIP53 has a balancing effect on rice immune response and growth.

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OsbZIP53 通过参与活性氧和水杨酸代谢负向调节水稻的免疫反应

碱性区/亮氨酸拉链（bZIP）转录因子在植物发育以及对非生物和生物胁迫的反应中发挥着重要作用。据报道，通过分析 APIP5-RNAi 转基因植株，OsbZIP53 可调控水稻对 Magnaporthe oryzae 的抗性。为了进一步研究OsbZIP53的生物学功能，我们利用CRISPR-Cas9编辑技术产生了osbzip53突变体，并过表达了OsbZIP53转基因植株。对表型、生理和转录数据的综合分析表明，敲除 OsbZIP53 不仅能通过诱导植物的超敏反应提高抗病性，还能通过水杨酸途径调节免疫反应。具体来说，敲除 OsbZIP53 会通过上调多个呼吸猝灭氧化酶同源基因（Osrboh 基因）促进活性氧的生成，从而增加 H2O2 的积累，并通过直接靶向 OsMYBS1 削弱 H2O2 的降解。此外，osbzip53突变体的生长受到严重影响，而OsbZIP53过表达株则表现出与野生型相似的表型，这表明OsbZIP53对水稻免疫反应和生长具有平衡作用。

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来源期刊

Rice Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

8.90

自引率

6.20%

发文量

审稿时长

40 weeks

期刊介绍： Rice Science is an international research journal sponsored by China National Rice Research Institute. It publishes original research papers, review articles, as well as short communications on all aspects of rice sciences in English language. Some of the topics that may be included in each issue are: breeding and genetics, biotechnology, germplasm resources, crop management, pest management, physiology, soil and fertilizer management, ecology, cereal chemistry and post-harvest processing.