Reference genome of Townsend’s big-eared bat, Corynorhinus townsendii

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2023-12-12 DOI:10.1093/jhered/esad078
Samantha L R Capel, Natalie M Hamilton, Devaughn Fraser, Merly Escalona, Oanh Nguyen, Samuel Sacco, Ruta Sahasrabudhe, William Seligmann, Juan M Vazquez, Peter H Sudmant, Michael L Morrison, Robert K Wayne, Michael R Buchalski
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Abstract

Townsend's big-eared bat, Corynorhinus townsendii, is a cave- and mine-roosting species found largely in western North American. Considered a species of conservation concern throughout much of its range, protection efforts would greatly benefit from understanding patterns of population structure, genetic diversity, and local adaptation. To facilitate such research, we present the first de novo genome assembly of C. townsendii as part of the California Conservation Genomics Project (CCGP). Pacific Biosciences HiFi long reads and Omni-C chromatin-proximity sequencing technology were used to produce a de novo genome assembly, consistent with the standard CCGP reference genome protocol. This assembly comprises 391 scaffolds spanning 2.1 Gb, represented by a scaffold N50 of 174.6 Mb, a contig N50 of 23.4 Mb, and a BUSCO completeness score of 96.6%. This high-quality genome will be a key tool for informed conservation and management of this vulnerable species in California and across its range.
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汤森氏大耳蝠(Corynorhinus townsendii)的参考基因组
汤森氏大耳蝠(Corynorhinus townsendii)是一种主要分布在北美西部的洞穴和矿井栖息物种。汤森氏大耳蝠在其分布的大部分地区都被认为是受保护的物种,了解其种群结构、遗传多样性和当地适应性的模式对保护工作大有裨益。为了促进此类研究,我们展示了作为加利福尼亚保护基因组学项目(CCGP)一部分的 C. Townsendii 的首个全新基因组组装。我们利用太平洋生物科学公司的 HiFi 长读数和 Omni-C 染色质邻近测序技术,按照标准的 CCGP 参考基因组方案,完成了从头基因组组装。该组装包括 391 个支架,跨度为 2.1 Gb,支架 N50 为 174.6 Mb,序列 N50 为 23.4 Mb,BUSCO 完整性得分为 96.6%。这一高质量的基因组将成为加利福尼亚州及其整个分布区保护和管理这一脆弱物种的重要工具。
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CiteScore
7.20
自引率
4.30%
发文量
567
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