Identification of distinct vascular mural cell populations during zebrafish embryonic development

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY Developmental Dynamics Pub Date : 2023-12-19 DOI:10.1002/dvdy.681
Sarah Colijn, Miku Nambara, Gracie Malin, Elena A. Sacchetti, Amber N. Stratman
{"title":"Identification of distinct vascular mural cell populations during zebrafish embryonic development","authors":"Sarah Colijn,&nbsp;Miku Nambara,&nbsp;Gracie Malin,&nbsp;Elena A. Sacchetti,&nbsp;Amber N. Stratman","doi":"10.1002/dvdy.681","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Mural cells are an essential perivascular cell population that associate with blood vessels and contribute to vascular stabilization and tone. In the embryonic zebrafish vasculature, <i>pdgfrb</i> and <i>tagln</i> are commonly used as markers for identifying pericytes and vascular smooth muscle cells. However, the overlapping and distinct expression patterns of these markers in tandem have not been fully described.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Here, we used the <i>Tg(pdgfrb:Gal4FF</i>; <i>UAS:RFP)</i> and <i>Tg(tagln:NLS-EGFP)</i> transgenic lines to identify single- and double-positive perivascular cell populations on the cranial, axial, and intersegmental vessels between 1 and 5 days postfertilization. From this comparative analysis, we discovered two novel regions of <i>tagln</i>-positive cell populations that have the potential to function as mural cell precursors. Specifically, we found that the hypochord—a reportedly transient structure—contributes to <i>tagln</i>-positive cells along the dorsal aorta. We also identified a unique mural cell progenitor population that resides along the midline between the neural tube and notochord and contributes to intersegmental vessel mural cell coverage.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Together, our findings highlight the variability and versatility of tracking both <i>pdgfrb</i> and <i>tagln</i> expression in mural cells of the developing zebrafish embryo and reveal unexpected embryonic cell populations that express <i>pdgfrb</i> and <i>tagln</i>.</p>\n </section>\n </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 5","pages":"519-541"},"PeriodicalIF":2.0000,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.681","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Dynamics","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.681","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background

Mural cells are an essential perivascular cell population that associate with blood vessels and contribute to vascular stabilization and tone. In the embryonic zebrafish vasculature, pdgfrb and tagln are commonly used as markers for identifying pericytes and vascular smooth muscle cells. However, the overlapping and distinct expression patterns of these markers in tandem have not been fully described.

Results

Here, we used the Tg(pdgfrb:Gal4FF; UAS:RFP) and Tg(tagln:NLS-EGFP) transgenic lines to identify single- and double-positive perivascular cell populations on the cranial, axial, and intersegmental vessels between 1 and 5 days postfertilization. From this comparative analysis, we discovered two novel regions of tagln-positive cell populations that have the potential to function as mural cell precursors. Specifically, we found that the hypochord—a reportedly transient structure—contributes to tagln-positive cells along the dorsal aorta. We also identified a unique mural cell progenitor population that resides along the midline between the neural tube and notochord and contributes to intersegmental vessel mural cell coverage.

Conclusion

Together, our findings highlight the variability and versatility of tracking both pdgfrb and tagln expression in mural cells of the developing zebrafish embryo and reveal unexpected embryonic cell populations that express pdgfrb and tagln.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
斑马鱼胚胎发育过程中不同血管壁细胞群的鉴定。
背景:壁细胞是一种重要的血管周围细胞群,它们与血管联系在一起,有助于血管的稳定和张力。在胚胎斑马鱼血管中,pdgfrb 和 tagln 通常被用作识别周细胞和血管平滑肌细胞的标记。然而,这些标记的重叠和不同的串联表达模式尚未得到充分描述:结果:在此,我们使用 Tg(pdgfrb:Gal4FF; UAS:RFP)和 Tg(tagln:NLS-EGFP)转基因品系鉴定了受精后 1 到 5 天内头颅、轴和节间血管上的单阳性和双阳性血管周围细胞群。通过比较分析,我们发现了两个标签ln阳性细胞群的新区域,它们有可能发挥壁细胞前体的功能。具体来说,我们发现背主动脉沿线的tagln阳性细胞主要来自下腹部--据报道这是一种瞬时结构。我们还发现了一个独特的壁细胞祖细胞群,它位于神经管和脊索之间的中线,有助于节间血管壁细胞的覆盖:总之,我们的研究结果突显了在发育中的斑马鱼胚胎壁细胞中追踪 pdgfrb 和 tagln 表达的可变性和多样性,并揭示了表达 pdgfrb 和 tagln 的意想不到的胚胎细胞群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
期刊最新文献
Review on pathogenesis and treatment of Alzheimer's disease. Cover Image Issue Information Editorial Editorial highlights
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1