Combined untargeted and targeted lipidomics approaches reveal potential biomarkers in type 2 diabetes mellitus cynomolgus monkeys

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES Journal of Medical Primatology Pub Date : 2023-12-11 DOI:10.1111/jmp.12688

Chao-Yang Tian, Qun-Hui Yang, Hai-Zhou Lv, Feng Yue, Fei-Fan Zhou

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Abstract

Background

The significantly increasing incidence of type 2 diabetes mellitus (T2DM) over the last few decades triggers the demands of T2DM animal models to explore the pathogenesis, prevention, and therapy of the disease. The altered lipid metabolism may play an important role in the pathogenesis and progression of T2DM. However, the characterization of molecular lipid species in fasting serum related to T2DM cynomolgus monkeys is still underrecognized.

Methods

Untargeted and targeted LC–mass spectrometry (MS)/MS-based lipidomics approaches were applied to characterize and compare the fasting serum lipidomic profiles of T2DM cynomolgus monkeys and the healthy controls.

Results

Multivariate analysis revealed that 196 and 64 lipid molecules differentially expressed in serum samples using untargeted and targeted lipidomics as the comparison between the disease group and healthy group, respectively. Furthermore, the comparative analysis of differential serum lipid metabolites obtained by untargeted and targeted lipidomics approaches, four common serum lipid species (phosphatidylcholine [18:0_22:4], lysophosphatidylcholine [14:0], phosphatidylethanolamine [PE] [16:1_18:2], and PE [18:0_22:4]) were identified as potential biomarkers and all of which were found to be downregulated. By analyzing the metabolic pathway, glycerophospholipid metabolism was associated with the pathogenesis of T2DM cynomolgus monkeys.

Conclusion

The study found that four downregulated serum lipid species could serve as novel potential biomarkers of T2DM cynomolgus monkeys. Glycerophospholipid metabolism was filtered out as the potential therapeutic target pathway of T2DM progression. Our results showed that the identified biomarkers may offer a novel tool for tracking disease progression and response to therapeutic interventions.

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非靶向和靶向脂质组学相结合的方法揭示了 2 型糖尿病猴的潜在生物标记物。

背景：过去几十年来，2 型糖尿病（T2DM）的发病率大幅上升，这引发了人们对 T2DM 动物模型的需求，以探索该疾病的发病机制、预防和治疗。脂质代谢的改变可能在 T2DM 的发病和进展过程中扮演重要角色。然而，与 T2DM 驴相关的空腹血清中分子脂质种类的表征仍未得到充分认识：方法：应用基于非靶向和靶向液相质谱（MS）/质谱（MS）的脂质组学方法表征和比较 T2DM 猴和健康对照组的空腹血清脂质组学特征：结果：多变量分析显示，以非靶向和靶向脂质组学作为疾病组和健康组的比较，血清样本中分别有196和64个脂质分子存在差异表达。此外，通过对非靶向和靶向脂质组学方法获得的差异血清脂质代谢物进行比较分析，发现四种常见的血清脂质（磷脂酰胆碱[18:0_22:4]、溶血磷脂酰胆碱[14:0]、磷脂酰乙醇胺[PE][16:1_18:2]和PE[18:0_22:4]）是潜在的生物标记物，且均出现下调。通过分析代谢途径，甘油磷脂代谢与 T2DM 猴的发病机制有关：结论：研究发现，四种下调的血清脂质可作为T2DM猴的新型潜在生物标志物。甘油磷脂代谢被筛选出作为 T2DM 进展的潜在治疗靶点途径。我们的研究结果表明，所发现的生物标志物可为跟踪疾病进展和对治疗干预措施的反应提供一种新的工具。

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.