Human breastmilk-derived Bifidobacterium longum subsp. infantis CCFM1269 regulates bone formation by the GH/IGF axis through PI3K/AKT pathway.

IF 12.2 1区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Gut Microbes Pub Date : 2024-01-01 Epub Date: 2023-12-20 DOI:10.1080/19490976.2023.2290344

Mengfan Ding, Bowen Li, Haiqin Chen, Dong Liang, R Paul Ross, Catherine Stanton, Jianxin Zhao, Wei Chen, Bo Yang

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Abstract

Bifidobacterium longum subsp. infantis is a prevalent member of the gut microbiota of breastfed infants. In this study, the effects of human breastmilk-derived B.longum subsp. infantis CCFM1269 on bone formation in developing BALB/c mice were investigated. Newborn female and male mice were assigned to control group (administered saline), CCFM11269 group (administered B. longum subsp. infantis CCFM1269, 1 × 10₉ CFU/mouse/day) and I5TI group (administered B. longum subsp. infantis I5TI, 1 × 10₉ CFU/mouse/day) from 1-week-old to 3-, 4- and 5-week old. B. longum subsp. infantis I5TI served as a negative control in this study. The results demonstrated that B. longum subsp. infantis CCFM1269 promoted bone formation in growing mice by modulating the composition of the gut microbiota and metabolites. The expression of genes and proteins in the PI3K/AKT pathway was stimulated by B. longum subsp. infantis CCFM1269 through the GH/IGF-1 axis in growing mice. This finding suggests B. longum subsp. infantis CCFM1269 may be useful for modulating bone metabolism during growth.

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人母乳来源的长双歧杆菌亚种 CCFM1269 通过 PI3K/AKT 通路调节 GH/IGF 轴的骨形成。

长双歧杆菌亚种（Bifidobacterium longum subsp. infantis）是母乳喂养婴儿肠道微生物群中的常见成员。在这项研究中，研究人员调查了母乳中长双歧杆菌亚种 CCFM1269 对发育中的 BALB/c 小鼠骨骼形成的影响。新生雌性和雄性小鼠从 1 周龄到 3、4 和 5 周龄分别被分配到对照组（给予生理盐水）、CCFM11269 组（给予婴儿长球菌亚种 CCFM1269，1 × 109 CFU/小鼠/天）和 I5TI 组（给予婴儿长球菌亚种 I5TI，1 × 109 CFU/小鼠/天）。本研究中的阴性对照为长球菌婴儿亚种 I5TI。结果表明，B. longum subsp. infantis CCFM1269 可通过调节肠道微生物群的组成和代谢物来促进生长期小鼠的骨形成。在生长期小鼠体内，婴儿龙球菌亚种 CCFM1269 通过 GH/IGF-1 轴刺激了 PI3K/AKT 通路中基因和蛋白质的表达。这一发现表明，婴儿龙球菌亚种 CCFM1269 可能有助于调节生长过程中的骨代谢。

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来源期刊

Gut Microbes Medicine-Microbiology (medical)

CiteScore

18.20

自引率

3.30%

发文量

196

审稿时长

10 weeks

期刊介绍： The intestinal microbiota plays a crucial role in human physiology, influencing various aspects of health and disease such as nutrition, obesity, brain function, allergic responses, immunity, inflammatory bowel disease, irritable bowel syndrome, cancer development, cardiac disease, liver disease, and more. Gut Microbes serves as a platform for showcasing and discussing state-of-the-art research related to the microorganisms present in the intestine. The journal emphasizes mechanistic and cause-and-effect studies. Additionally, it has a counterpart, Gut Microbes Reports, which places a greater focus on emerging topics and comparative and incremental studies.