Electroacupuncture protects against the striatum of ischemia stroke by inhibiting the HMGB1/RAGE/p-JNK signaling pathways

IF 2.7 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of chemical neuroanatomy Pub Date : 2023-12-19 DOI:10.1016/j.jchemneu.2023.102376

Zeyin Nie, Chenying Hu, Huachun Miao, Feng Wu

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Abstract

The striatum (Str) is injured 20 min after permanent ischemic stroke, leading to neurological deficits. Here, we aimed to explore the effect of electroacupuncture (EA) on ischemic stroke and elucidate the possible underlying mechanism. Rat permanent middle cerebral artery occlusion (pMCAO) model, EA treatment, sham-EA (SEA) treatment, beam-balance test, hematoxylin and eosin (HE) staining, Nissl staining, immunofluorescence staining, and Western blot were used to investigate the role of EA in pMCAO. The results showed that balance ability and motor coordination were obviously injured after pMCAO. EA improved balance ability and motor coordination in pMCAO rats. EA reduced striatal injury by reducing the expression of high-mobility group box 1(HMGB1)/receptor for advanced glycation end products (RAGE)/phosphorylated C-Jun N-terminal kinase (p-JNK), whereas SEA did not. Thus, EA plays a neuroprotective role during pMCAO injury, which may be related to the inhibition of HMGB1/RAGE/p-JNK expression.

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电针通过抑制 HMGB1/RAGE/p-JNK 信号通路保护缺血性脑卒中的纹状体

永久性缺血性脑卒中发生后20分钟，脑纹状体（Str）就会受到损伤，导致神经功能缺损。在此，我们旨在探讨电针（EA）对缺血性脑卒中的影响，并阐明其可能的内在机制。实验采用大鼠永久性大脑中动脉闭塞（pMCAO）模型、EA治疗、假EA（SEA）治疗、横梁平衡试验、苏木精和伊红（HE）染色、Nissl染色、免疫荧光染色、Western blot等方法研究EA在pMCAO中的作用。结果显示，pMCAO后平衡能力和运动协调能力明显受损。EA改善了pMCAO大鼠的平衡能力和运动协调能力。EA通过减少高迁移率组盒1（HMGB1）/高级糖化终产物受体（RAGE）/磷酸化C-Jun N-末端激酶（p-JNK）的表达来减轻纹状体损伤，而SEA则没有。因此，EA在pMCAO损伤中起到神经保护作用，这可能与抑制HMGB1/RAGE/p-JNK的表达有关。

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来源期刊

Journal of chemical neuroanatomy 医学-神经科学

CiteScore

4.50

自引率

3.60%

发文量

审稿时长

62 days

期刊介绍： The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.