Tetrahedral serial multiview microscopy and image fusion for improved resolution and extent in stained zebrafish embryos

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY Developmental Dynamics Pub Date : 2023-12-22 DOI:10.1002/dvdy.683
Johanna B. Kroll, Anna Cha, Alon Oyler-Yaniv, Talley Lambert, Ian A. Swinburne, Andrew Murphy, Sean G. Megason
{"title":"Tetrahedral serial multiview microscopy and image fusion for improved resolution and extent in stained zebrafish embryos","authors":"Johanna B. Kroll,&nbsp;Anna Cha,&nbsp;Alon Oyler-Yaniv,&nbsp;Talley Lambert,&nbsp;Ian A. Swinburne,&nbsp;Andrew Murphy,&nbsp;Sean G. Megason","doi":"10.1002/dvdy.683","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Spatial mapping on the single-cell level over the whole organism can uncover roles of molecular players involved in vertebrate development. Custom microscopes have been developed that use multiple objectives to view a sample from multiple views at the same time. Such multiview imaging approaches can improve resolution and uniformity of image quality as well as allow whole embryos to be imaged (Swoger et al., <i>Opt Express</i>, 2007;15(13):8029). However, multiview imaging is highly restricted to specialized equipment requiring multiple objectives or sample rotation with automated hardware.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Our approach uses a standard single-objective confocal microscope to perform serial multiview imaging. Multiple views are imaged sequentially by mounting the fixed sample in an agarose tetrahedron that is manually rotated in between imaging each face. Computational image fusion allows for a joint 3D image to be created from multiple tiled Z-stacks acquired from different angles. The resulting fused image has improved resolution and imaging extent.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>With this technique, multiview imaging can be performed on a variety of common single-objective microscopes to allow for whole-embryo, high-resolution imaging.</p>\n </section>\n </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 7","pages":"690-704"},"PeriodicalIF":2.0000,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Dynamics","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.683","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background

Spatial mapping on the single-cell level over the whole organism can uncover roles of molecular players involved in vertebrate development. Custom microscopes have been developed that use multiple objectives to view a sample from multiple views at the same time. Such multiview imaging approaches can improve resolution and uniformity of image quality as well as allow whole embryos to be imaged (Swoger et al., Opt Express, 2007;15(13):8029). However, multiview imaging is highly restricted to specialized equipment requiring multiple objectives or sample rotation with automated hardware.

Results

Our approach uses a standard single-objective confocal microscope to perform serial multiview imaging. Multiple views are imaged sequentially by mounting the fixed sample in an agarose tetrahedron that is manually rotated in between imaging each face. Computational image fusion allows for a joint 3D image to be created from multiple tiled Z-stacks acquired from different angles. The resulting fused image has improved resolution and imaging extent.

Conclusion

With this technique, multiview imaging can be performed on a variety of common single-objective microscopes to allow for whole-embryo, high-resolution imaging.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
四面体序列多视图显微镜和图像融合技术提高了染色斑马鱼胚胎的分辨率和范围。
背景:在单细胞水平上绘制整个生物体的空间图谱可以发现参与脊椎动物发育的分子角色。目前已开发出定制显微镜,可使用多个物镜同时从多个视角观察样本。这种多视角成像方法可提高图像质量的分辨率和均匀性,并可对整个胚胎进行成像(Swoger 等人,《光学快报》,2007 年;15(13):8029)。然而,多视角成像在很大程度上受限于需要多个物镜的专用设备或自动硬件的样本旋转:结果:我们的方法使用标准的单目标共聚焦显微镜来执行序列多视图成像。通过将固定样本安装在琼脂糖四面体中,在每个面成像之间手动旋转四面体,对多个视图进行顺序成像。通过计算图像融合,可以从不同角度获取的多个平铺 Z 叠加图像创建一个联合三维图像。融合后的图像具有更高的分辨率和成像范围:利用这项技术,可以在各种常见的单目标显微镜上进行多视角成像,从而实现全胚胎高分辨率成像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
期刊最新文献
Review on pathogenesis and treatment of Alzheimer's disease. Cover Image Issue Information Editorial Editorial highlights
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1