Tae-Young Gil, Junkyu Park, Yea-Jin Park, Hyo-Jung Kim, Divina C Cominguez, Hyo-Jin An
{"title":"Drynaria rhizome water extract alleviates high‑fat diet‑induced obesity in mice.","authors":"Tae-Young Gil, Junkyu Park, Yea-Jin Park, Hyo-Jung Kim, Divina C Cominguez, Hyo-Jin An","doi":"10.3892/mmr.2023.13153","DOIUrl":null,"url":null,"abstract":"<p><p>Drynaria rhizome is a herbal medicine used for strengthening bones and treating bone diseases in East Asia. Although obesity is considered to benefit bone formation, it has been revealed that visceral fat accumulation can promote osteoporosis. Given the complex relationship between bone metabolism and obesity, bone‑strengthening medicines should be evaluated while considering the effects of obesity. The present study investigated the effects of Drynaria rhizome extract (DRE) on high‑fat diet (HFD)‑induced obese mice. DRE was supplemented with the HFD. Body weight, food intake, the expression levels of lipogenesis transcription factors, including sterol regulatory element binding protein (SREBP)‑1, peroxisome proliferator‑activated receptor (PPAR)‑γ and adenosine monophosphate‑activated protein kinase (AMPK)‑α, and AMPK activation were evaluated. Mice fed DRE and a HFD exhibited reduced body weight without differences in food intake compared with those in the HFD group. Furthermore, DRE; upregulated AMPK‑α of epididymal one; down‑regulated SREBP‑1 and PPAR‑γ, as determined using western blotting and quantitative polymerase chain reaction, respectively. Decreased lipid accumulation were observed in both fat pad and liver of HFD‑fed mice, which were suppressed by DRE treatment. These results demonstrated the potential of DRE as a dietary natural product for strengthening bones and managing obesity.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"29 2","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10784730/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular medicine reports","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3892/mmr.2023.13153","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/22 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
Abstract
Drynaria rhizome is a herbal medicine used for strengthening bones and treating bone diseases in East Asia. Although obesity is considered to benefit bone formation, it has been revealed that visceral fat accumulation can promote osteoporosis. Given the complex relationship between bone metabolism and obesity, bone‑strengthening medicines should be evaluated while considering the effects of obesity. The present study investigated the effects of Drynaria rhizome extract (DRE) on high‑fat diet (HFD)‑induced obese mice. DRE was supplemented with the HFD. Body weight, food intake, the expression levels of lipogenesis transcription factors, including sterol regulatory element binding protein (SREBP)‑1, peroxisome proliferator‑activated receptor (PPAR)‑γ and adenosine monophosphate‑activated protein kinase (AMPK)‑α, and AMPK activation were evaluated. Mice fed DRE and a HFD exhibited reduced body weight without differences in food intake compared with those in the HFD group. Furthermore, DRE; upregulated AMPK‑α of epididymal one; down‑regulated SREBP‑1 and PPAR‑γ, as determined using western blotting and quantitative polymerase chain reaction, respectively. Decreased lipid accumulation were observed in both fat pad and liver of HFD‑fed mice, which were suppressed by DRE treatment. These results demonstrated the potential of DRE as a dietary natural product for strengthening bones and managing obesity.
旱金莲根茎在东亚是一种用于强化骨骼和治疗骨病的草药。虽然肥胖被认为有利于骨骼的形成,但研究发现内脏脂肪堆积会促进骨质疏松。鉴于骨代谢与肥胖之间的复杂关系,在评估健骨药物时应同时考虑肥胖的影响。本研究调查了旱莲草根茎提取物(DRE)对高脂饮食(HFD)诱导的肥胖小鼠的影响。在高脂饮食中添加 DRE。对体重、食物摄入量、脂肪生成转录因子(包括固醇调节元件结合蛋白(SREBP)-1、过氧化物酶体增殖激活受体(PPAR)-γ和单磷酸腺苷激活蛋白激酶(AMPK)-α)的表达水平以及AMPK激活情况进行了评估。与高饱和脂肪酸组的小鼠相比,喂食 DRE 和高饱和脂肪酸组的小鼠体重下降,但食物摄入量没有差异。此外,使用 Western 印迹和定量聚合酶链反应分别测定,DRE 上调了附睾中的 AMPK-α,下调了 SREBP-1 和 PPAR-γ。高密度脂蛋白胆固醇喂养小鼠的脂肪垫和肝脏中均观察到脂质积累减少,而 DRE 治疗可抑制脂质积累。这些结果证明了 DRE 作为一种膳食天然产品在强化骨骼和控制肥胖方面的潜力。
期刊介绍:
Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.