Redescription, molecular characterisation and Wolbachia endosymbionts of Mansonella (Tupainema) dunni (Mullin & Orihel, 1972) (Spirurida: Onchocercidae) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia
Ahmad Syihan Mat Udin , Shigehiko Uni , Jules Rodrigues , Coralie Martin , Kerstin Junker , Takeshi Agatsuma , Van Lun Low , Weerachai Saijuntha , Hasmahzaiti Omar , Nur Afiqah Zainuri , Masako Fukuda , Makoto Matsubayashi , Daisuke Kimura , Hiroyuki Takaoka , Rosli Ramli
{"title":"Redescription, molecular characterisation and Wolbachia endosymbionts of Mansonella (Tupainema) dunni (Mullin & Orihel, 1972) (Spirurida: Onchocercidae) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia","authors":"Ahmad Syihan Mat Udin , Shigehiko Uni , Jules Rodrigues , Coralie Martin , Kerstin Junker , Takeshi Agatsuma , Van Lun Low , Weerachai Saijuntha , Hasmahzaiti Omar , Nur Afiqah Zainuri , Masako Fukuda , Makoto Matsubayashi , Daisuke Kimura , Hiroyuki Takaoka , Rosli Ramli","doi":"10.1016/j.crpvbd.2023.100154","DOIUrl":null,"url":null,"abstract":"<div><p>The genus <em>Mansonella</em> Faust, 1929 includes 29 species, mainly parasites of platyrrhine monkeys in South America and anthropoid apes in Africa. In Malaysia, <em>Mansonella</em> (<em>Tupainema</em>) <em>dunni</em> (Mullin & Orihel, 1972) was described from the common treeshrew <em>Tupaia glis</em> Diard & Duvaucel (Scandentia). In a recent classification of the genus <em>Mansonella</em>, seven subgenera were proposed, with <em>M</em>. (<em>Tup</em>.) <em>dunni</em> as a monotypic species in the subgenus <em>Tupainema</em>. In this study, we collected new material of <em>M</em>. (<em>Tup</em>.) <em>dunni</em> from common treeshrews in Peninsular Malaysia and redescribed the morphological features of this species. We found that <em>M</em>. (<em>Tup</em>.) <em>dunni</em> differs from <em>M</em>. (<em>Cutifilaria</em>) <em>perforata</em> Uni et al., 2004 from sika deer <em>Cervus nippon</em> (Cetartiodactyla) in Japan, with regards to morphological features and predilection sites in their respective hosts. Based on multi-locus sequence analyses, we examined the molecular phylogeny of <em>M</em>. (<em>Tup</em>.) <em>dunni</em> and its <em>Wolbachia</em> genotype. Species of the genus <em>Mansonella</em> grouped monophyletically in clade ONC5 and <em>M</em>. (<em>Tup</em>.) <em>dunni</em> was placed in the most derived position within this genus. <em>Mansonella</em> (<em>Tup</em>.) <em>dunni</em> was closely related to <em>M</em>. (<em>M</em>.) <em>ozzardi</em> (Manson, 1897) from humans in Central and South America, and most distant from <em>M</em>. (<em>C</em>.) <em>perforata</em>. The calculated p-distances between the <em>cox</em>1 gene sequences for <em>M</em>. (<em>Tup</em>.) <em>dunni</em> and its congeners were 13.09% for <em>M</em>. (<em>M.</em>) <em>ozzardi</em> and 15.6–16.15% for <em>M</em>. (<em>C</em>.) <em>perforata</em>. The molecular phylogeny of <em>Mansonella</em> spp. thus corroborates their morphological differences. We determined that <em>M</em>. (<em>Tup</em>.) <em>dunni</em> harbours <em>Wolbachia</em> endosymbionts of the supergroup F genotype, in keeping with all other <em>Mansonella</em> species screened to date.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667114X23000420/pdfft?md5=1ce8aff825d5351c592fe5979160d946&pid=1-s2.0-S2667114X23000420-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current research in parasitology & vector-borne diseases","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667114X23000420","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The genus Mansonella Faust, 1929 includes 29 species, mainly parasites of platyrrhine monkeys in South America and anthropoid apes in Africa. In Malaysia, Mansonella (Tupainema) dunni (Mullin & Orihel, 1972) was described from the common treeshrew Tupaia glis Diard & Duvaucel (Scandentia). In a recent classification of the genus Mansonella, seven subgenera were proposed, with M. (Tup.) dunni as a monotypic species in the subgenus Tupainema. In this study, we collected new material of M. (Tup.) dunni from common treeshrews in Peninsular Malaysia and redescribed the morphological features of this species. We found that M. (Tup.) dunni differs from M. (Cutifilaria) perforata Uni et al., 2004 from sika deer Cervus nippon (Cetartiodactyla) in Japan, with regards to morphological features and predilection sites in their respective hosts. Based on multi-locus sequence analyses, we examined the molecular phylogeny of M. (Tup.) dunni and its Wolbachia genotype. Species of the genus Mansonella grouped monophyletically in clade ONC5 and M. (Tup.) dunni was placed in the most derived position within this genus. Mansonella (Tup.) dunni was closely related to M. (M.) ozzardi (Manson, 1897) from humans in Central and South America, and most distant from M. (C.) perforata. The calculated p-distances between the cox1 gene sequences for M. (Tup.) dunni and its congeners were 13.09% for M. (M.) ozzardi and 15.6–16.15% for M. (C.) perforata. The molecular phylogeny of Mansonella spp. thus corroborates their morphological differences. We determined that M. (Tup.) dunni harbours Wolbachia endosymbionts of the supergroup F genotype, in keeping with all other Mansonella species screened to date.