Gluten/chitosan Nanofiber-based Films Activated by Cationic Inulin-coated Betanin and Carvone Co-loaded Nanoliposomes: Preparation and Characterization

IF 2.8 4区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food Biophysics Pub Date : 2023-12-21 DOI:10.1007/s11483-023-09820-8
Sajed Amjadi, Hadi Almasi, Hamed Hamishehkar, Mohammad Alizadeh Khaledabad, Loong-Tak Lim, Sara Gholizadeh
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Abstract

In this research, dual-activated wheat gluten-based nanocomposite active films reinforced by chitosan nanofibers (CHNF) was developed, as well as co-loaded with betanin (BET) and carvone (CAR) for application in food packaging. Free and cationic inulin-coated nanoliposomal forms of BET/CAR were incorporated into the gluten films at 0, 5, and 10% (w/w) gluten concentrations. Fourier-transform infrared spectroscopy was used to detect the formation of new hydrogen bonds between gluten, CHNF, and nanoliposomes (NLPs). Differential scanning calorimetry analysis revealed that the addition of free BET/CAR reduced the endothermic transition temperature of films as compared to the unfortified film; however, the addition of NLPs had no effect on the thermal profile. Similar changes were observed in the crystallinity of films as determined by X-ray powder diffraction analysis. FE-SEM results showed that the incorporation of CHNF and BET/CAR NLPs did not alter the morphology of films but the free BET/CAR induced aggregates formation on the films' surface. CHNF addition enhanced the mechanical, water barrier, and wettability of films. Among the films tested, those containing BET/CAR NLPs had the highest antioxidant potential. However, the encapsulated BET/CAR in inulin-coated NLPs had an improved antimicrobial activity. The films containing the nanoliposomal form of BET/CAR indicated a controlled release behavior.

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阳离子菊粉包被的贝塔宁和卡文酮共负载纳米脂质体活化的面筋/壳聚糖纳米纤维基薄膜:制备与表征
本研究开发了由壳聚糖纳米纤维(CHNF)增强的双活性小麦面筋基纳米复合活性薄膜,并共同负载了甜菜宁(BET)和卡沃酮(CAR),用于食品包装。BET/CAR的游离型和阳离子菊粉包被型纳米脂质体以0、5和10%(重量比)的麸质浓度被加入到麸质薄膜中。傅立叶变换红外光谱用于检测麸质、CHNF 和纳米脂质体 (NLP) 之间新氢键的形成。差示扫描量热分析表明,与未强化的薄膜相比,添加游离 BET/CAR 降低了薄膜的内热转变温度;然而,添加 NLPs 对热曲线没有影响。通过 X 射线粉末衍射分析确定的薄膜结晶度也发生了类似的变化。FE-SEM 结果表明,加入 CHNF 和 BET/CAR NLPs 不会改变薄膜的形态,但游离的 BET/CAR 会在薄膜表面形成聚集体。CHNF 的添加增强了薄膜的机械性能、水阻隔性和润湿性。在测试的薄膜中,含有 BET/CAR NLPs 的薄膜具有最高的抗氧化潜力。然而,在菊粉包覆的 NLP 中封装 BET/CAR 的抗菌活性更高。含有 BET/CAR 纳米脂质体形式的薄膜具有控释特性。
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来源期刊
Food Biophysics
Food Biophysics 工程技术-食品科技
CiteScore
5.80
自引率
3.30%
发文量
58
审稿时长
1 months
期刊介绍: Biophysical studies of foods and agricultural products involve research at the interface of chemistry, biology, and engineering, as well as the new interdisciplinary areas of materials science and nanotechnology. Such studies include but are certainly not limited to research in the following areas: the structure of food molecules, biopolymers, and biomaterials on the molecular, microscopic, and mesoscopic scales; the molecular basis of structure generation and maintenance in specific foods, feeds, food processing operations, and agricultural products; the mechanisms of microbial growth, death and antimicrobial action; structure/function relationships in food and agricultural biopolymers; novel biophysical techniques (spectroscopic, microscopic, thermal, rheological, etc.) for structural and dynamical characterization of food and agricultural materials and products; the properties of amorphous biomaterials and their influence on chemical reaction rate, microbial growth, or sensory properties; and molecular mechanisms of taste and smell. A hallmark of such research is a dependence on various methods of instrumental analysis that provide information on the molecular level, on various physical and chemical theories used to understand the interrelations among biological molecules, and an attempt to relate macroscopic chemical and physical properties and biological functions to the molecular structure and microscopic organization of the biological material.
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