Farklı Malathion Konsantrasyonlarının Kültürel Koşullarda Alg Büyümesi Üzerine Etkileri

E. Soylu, Bengü Temizel
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Abstract

Malathion is one of the insecticides commonly used to control hazelnut pests in hazelnut orchards in Giresun region. This insecticide pollutes lakes, rivers and sea waters by drifting from the soil with rain, flood and snow waters. In this study, Scenedesmus sp. cultures were prepared in BG-11 medium for use in laboratory experiments. It was aimed to determine the change in algal growth due to the increase in malathion concentration applied to these cultures. Growth of strains in cultures treated with malathion at doses of 0.05 mg/L, 0.5 mg/L, 1 mg/L, 5 mg/L and 10 mg/L were compared with those grown in non-malathion cultures. In addition, pH was measured and chlorophyll-a values were also calculated for the control group and the cultures to which malathion was added during the study. Cell number showed different changes over time according to pesticide concentrations. The highest number of cells was 3.61x106 cells/ml at 10 mg/L dose at the end of 24th hour and the lowest number of cells was 2.05x106 cells/ml at 10 mg/L dose at the end of 48th hour. pH values did not fluctuate much and generally decreased at the end of 96th hour. The lowest chlorophyll-a was calculated as 0.35 µg/L at 96th hour. As a result, it was determined that the doses studied negatively affected algal growth, although not too much.
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不同马拉硫磷浓度对培养条件下藻类生长的影响
马拉硫磷是吉雷松地区榛子果园常用的杀虫剂之一,用于控制榛子害虫。这种杀虫剂会随雨水、洪水和雪水从土壤中漂流出来,污染湖泊、河流和海水。本研究在 BG-11 培养基中制备了 Scenedesmus sp.目的是确定藻类生长因马拉硫磷浓度增加而发生的变化。与未使用马拉硫磷的培养物中的菌株生长情况进行了比较,马拉硫磷的剂量分别为 0.05 毫克/升、0.5 毫克/升、1 毫克/升、5 毫克/升和 10 毫克/升。此外,还测量了对照组和研究期间添加马拉硫磷的培养物的 pH 值,并计算了叶绿素-a 值。根据农药浓度的不同,细胞数量随时间发生了不同的变化。在第 24 小时结束时,10 毫克/升剂量的细胞数最高,为 3.61x106 个/毫升;在第 48 小时结束时,10 毫克/升剂量的细胞数最低,为 2.05x106 个/毫升。在第 96 小时,叶绿素-a 最低值为 0.35 微克/升。因此,可以确定所研究的剂量对藻类的生长有负面影响,但影响不大。
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