IDENTIFICATION OF VIRAL PATHOGENS BY SEROLOGICAL TECHNIQUES AND MOLECULAR DIAGNOSTIC TECHNIQUES IN APRICOT, PEACH AND ALMOND

Abstract

In order to obtain plants of the biological category Pre-basic, plants of the genotypes ꞌOlimpꞌ and ꞌDe Valuꞌ in apricot, ꞌRalucaꞌ, ꞌMonicaꞌ, ꞌTomis 1ꞌ in peach, ꞌMirelaꞌ and ꞌVeronicaꞌ in almond were evaluated for the presence of virus-type harmful organisms. Diagnosis was made by the serological DAS-ELISA method for ACLSV, ApMV, PPV, PDV, PNRSV and SLRSV and by the molecular RT-PCR test for ESFY, ApLV and PLMVd. No positive samples were found in apricot. In peach the viruses were identified in ꞌMonicaꞌ: ApLV in 57.15% of the plants tested, PNRSV in 14.29% and PLMVd in 7.15% of the plants. Two of the positive plants had mixed infections produced by PNRSV +ApLV and PNRSV +PLMVd. In peach genotype ꞌTomis 1ꞌ, 23.08 % plants were found infected with ApMV, 7.7 % with ACLSV and 7.7 % with PPV. ACLSV and PPV infections were identified in the same plant. In almond the diagnosis revealed a mixed infection with ACLSV + ApMV (7.7 % of plants from ꞌMirelaꞌ variety).