3D-printed cassette integrated with paper-based aptasensor for the construction of next-generation sensing tool to detect dengue virus towards plaspertronix-commercialization

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology Biosensors and Bioelectronics: X Pub Date : 2023-12-21 DOI:10.1016/j.biosx.2023.100431

MohdRahil Hasan, Pradakshina Sharma, Saumitra Singh, Annu Mishra, Zaira Azmi, Jagriti Narang

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Abstract

The present study describes the creation of a 3D printed cassette named “3DP-PAC”, integrated to an electrochemical-aptasensor for the detection of dengue virus. It consists of an electrode cassette printed from a PLA non-conductive filament that provides a sophisticated design & support system to the delicate conductive paper-electrode. Chemically synthesized GO/ZnO-NC was used, which increases the sensor's sensitivity by accelerating the flow of electrons transferring. DENV DNA-Aptamer specifically binds to its target antigen of DENV, confirming its selectivity and demonstrating no cross reactivity with chikungunya virus antigen (CHIKV–Ag). The current study developed a 3D-based aptasensor for detecting dengue virus at a low level of detection (0.1 μg/ml). In human serum, the established platform performed well. This study paves the way for the manufacture of next-generation electrochemical biosensors utilizing 3D printing technology, with possible consequences for healthcare applications on the edge of commercialization.

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三维打印盒与纸质灵敏度传感器集成，用于构建检测登革热病毒的下一代传感工具，实现 plaspertronix 商品化

本研究描述了一种名为 "3DP-PAC "的三维打印盒的制作过程，它与用于检测登革热病毒的电化学传感器集成在一起。它由聚乳酸（PLA）非导电长丝打印而成的电极盒组成，为精致的导电纸电极提供了复杂的设计和支持系统。它使用了化学合成的 GO/ZnO-NC，通过加速电子转移流来提高传感器的灵敏度。DENV DNA-吻合器能特异性地与 DENV 目标抗原结合，证实了其选择性，并且与基孔肯雅病毒抗原（CHIKV-Ag）没有交叉反应。目前的研究开发了一种基于三维的适配传感器，可在低检测水平（0.1 μg/ml）下检测登革热病毒。在人类血清中，所建立的平台表现良好。这项研究为利用三维打印技术制造下一代电化学生物传感器铺平了道路，可能会对处于商业化边缘的医疗保健应用产生影响。

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.