Biosensors and Bioelectronics: X最新文献

Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-16 DOI: 10.1016/j.biosx.2025.100621

Ahmed A. Shalaby , Asmaa Salah , Akihiko Ishida , Masatoshi Maeki , Manabu Tokeshi

Breast cancer is the most common cancer type in women and it has the highest probability of developing into invasive cancer. Early detection of breast cancer is crucial to reduce the disease burden and decrease the mortality rate. Detection of cancer biomarkers is an attractive non-invasive way to implement early diagnosis and follow-up. Colorimetric enzyme-linked immunosorbent assay (ELISA) is one of the most common techniques used for the detection of cancer biomarkers. However, it requires a long incubation time and a large reagent volume, and it has low sensitivity. Here, we propose use of a paper disc in a 3D printed microplate hybrid microfluidic device for ultrasensitive paper-based bioluminescence ELISA for detection of HER2 breast cancer biomarker. Chromatographic paper discs are good substrates for fast immobilization of capture antibody without making any surface modification and they can be replaced with new discs to reuse the 3D printed microplate. The 3D printed microplate has microvalves in the bottom of the wells, so it can stop flow of the reagents for the desired incubation time and it allows the washing solution to flow vertically and drain onto an adsorption pad which increases the washing efficiency. NanoLuc luciferase was used as a label for the detection antibody to achieve the highest sensitivity. Bioluminescence sandwich ELISA for HER2 detection was performed using the hybrid device in just 20 min and the limit of detection was 1.3 fg/mL which is more than 10⁴-fold better than commercial ELISA kits for HER2.

{"title":"Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker","authors":"Ahmed A. Shalaby , Asmaa Salah , Akihiko Ishida , Masatoshi Maeki , Manabu Tokeshi","doi":"10.1016/j.biosx.2025.100621","DOIUrl":"10.1016/j.biosx.2025.100621","url":null,"abstract":"<div><div>Breast cancer is the most common cancer type in women and it has the highest probability of developing into invasive cancer. Early detection of breast cancer is crucial to reduce the disease burden and decrease the mortality rate. Detection of cancer biomarkers is an attractive non-invasive way to implement early diagnosis and follow-up. Colorimetric enzyme-linked immunosorbent assay (ELISA) is one of the most common techniques used for the detection of cancer biomarkers. However, it requires a long incubation time and a large reagent volume, and it has low sensitivity. Here, we propose use of a paper disc in a 3D printed microplate hybrid microfluidic device for ultrasensitive paper-based bioluminescence ELISA for detection of HER2 breast cancer biomarker. Chromatographic paper discs are good substrates for fast immobilization of capture antibody without making any surface modification and they can be replaced with new discs to reuse the 3D printed microplate. The 3D printed microplate has microvalves in the bottom of the wells, so it can stop flow of the reagents for the desired incubation time and it allows the washing solution to flow vertically and drain onto an adsorption pad which increases the washing efficiency. NanoLuc luciferase was used as a label for the detection antibody to achieve the highest sensitivity. Bioluminescence sandwich ELISA for HER2 detection was performed using the hybrid device in just 20 min and the limit of detection was 1.3 fg/mL which is more than 10<sup>4</sup>-fold better than commercial ELISA kits for HER2.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100621"},"PeriodicalIF":10.61,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longitudinal tracking of chronic inflammation through Calprotectin and Interleukin-6 using a sweat wearable device

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-05 DOI: 10.1016/j.biosx.2025.100622

Sarah Shahub , Annapoorna Ramasubramanya , Preeti Singh , Ruchita Mahesh Kumar , Kai-Chun Lin , Sriram Muthukumar , Shalini Prasad

This work demonstrates a continuous, noninvasive two-plex electrochemical biosensor for the measurement of inflammation in sweat via disease-specific and general markers Calprotectin and Interleukin-6 (IL-6), respectively. Sensor functionalization and sensor stability was characterized through Fourier Transform Infrared (FTIR) spectroscopy. Sensor stability was characterized through open circuit potential and electrochemical impedance spectroscopy (EIS). On-body stability was demonstrated through relative humidity and temperature measurements of the sensor-skin interface.

Calprotectin and IL-6 were measured in sweat over 2 days from 2 chronically inflamed subjects and 10 healthy subjects to characterize dual sweat expression of the markers and investigate diurnal patterns of expression across the two groups. Sweat Calprotectin was continuously tracked over a 40-h period for chronically inflamed and healthy subjects with different inflammatory activity and treatments. Sensor measurements were recorded continuously with a sampling rate of 1–1.5 min.

Significantly higher sweat Calprotectin and higher median sweat Calprotectin expression was observed in the morning-afternoon than in the evening among inflamed and healthy subjects, respectively. Higher median sweat IL-6 was observed in inflamed individuals in the morning-afternoon, while higher median sweat IL-6 was observed in healthy individuals in the evening. Temporal results of sweat Calprotectin tracking demonstrate higher basal Calprotectin in an unmedicated over a medicated inflamed subject, and higher basal Calprotectin of inflamed subjects over a healthy subject.

Calprotectin and IL-6 demonstrated a strong positive linear relationship in sweat. Diurnal patterns were observed in the sweat of inflamed and healthy individuals, and continuous tracking of disease-specific inflammation through sweat Calprotectin was demonstrated.

{"title":"Longitudinal tracking of chronic inflammation through Calprotectin and Interleukin-6 using a sweat wearable device","authors":"Sarah Shahub , Annapoorna Ramasubramanya , Preeti Singh , Ruchita Mahesh Kumar , Kai-Chun Lin , Sriram Muthukumar , Shalini Prasad","doi":"10.1016/j.biosx.2025.100622","DOIUrl":"10.1016/j.biosx.2025.100622","url":null,"abstract":"<div><div>This work demonstrates a continuous, noninvasive two-plex electrochemical biosensor for the measurement of inflammation in sweat via disease-specific and general markers Calprotectin and Interleukin-6 (IL-6), respectively. Sensor functionalization and sensor stability was characterized through Fourier Transform Infrared (FTIR) spectroscopy. Sensor stability was characterized through open circuit potential and electrochemical impedance spectroscopy (EIS). On-body stability was demonstrated through relative humidity and temperature measurements of the sensor-skin interface.</div><div>Calprotectin and IL-6 were measured in sweat over 2 days from 2 chronically inflamed subjects and 10 healthy subjects to characterize dual sweat expression of the markers and investigate diurnal patterns of expression across the two groups. Sweat Calprotectin was continuously tracked over a 40-h period for chronically inflamed and healthy subjects with different inflammatory activity and treatments. Sensor measurements were recorded continuously with a sampling rate of 1–1.5 min.</div><div>Significantly higher sweat Calprotectin and higher median sweat Calprotectin expression was observed in the morning-afternoon than in the evening among inflamed and healthy subjects, respectively. Higher median sweat IL-6 was observed in inflamed individuals in the morning-afternoon, while higher median sweat IL-6 was observed in healthy individuals in the evening. Temporal results of sweat Calprotectin tracking demonstrate higher basal Calprotectin in an unmedicated over a medicated inflamed subject, and higher basal Calprotectin of inflamed subjects over a healthy subject.</div><div>Calprotectin and IL-6 demonstrated a strong positive linear relationship in sweat. Diurnal patterns were observed in the sweat of inflamed and healthy individuals, and continuous tracking of disease-specific inflammation through sweat Calprotectin was demonstrated.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100622"},"PeriodicalIF":10.61,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic Cell Microscopy: A new modality for visualizing cells using microfluidic impedance cytometry and generative artificial intelligence

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-04-05 DOI: 10.1016/j.biosx.2025.100619

Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard

This study introduces a novel approach to cancer cell imaging by integrating microfluidic sensor technology with artificial intelligence (AI). We developed a custom microfluidic device with polydimethylsiloxane (PDMS) microchannels and integrated electrodes to capture electrical impedance data. The device was fabricated using photolithography, electron beam evaporation, and lift-off techniques. Instead of traditional imaging methods, electrical impedance signals were used to reconstruct cell images. A generative AI model with eight hidden layers processed 191 impedance values to accurately reconstruct the shapes of cancer cells and control beads. Our approach successfully reconstructed images of MDA-MB-231 breast cancer cells, HeLa cells, and beads, achieving 91 % accuracy on the test dataset. Validation using the Structural Similarity Index (SSI) and Mean Structural Similarity Index (MSSIM) produced scores of 0.97 for breast cancer cells and 0.93 for beads, confirming the high precision of this method. This label-free, impedance-based imaging offers a promising solution for cancer diagnostics by accurately reconstructing cell shapes and distinguishing cell types, particularly in point-of-care applications.

{"title":"Ionic Cell Microscopy: A new modality for visualizing cells using microfluidic impedance cytometry and generative artificial intelligence","authors":"Mahtab Kokabi , Gulam M. Rather , Mehdi Javanmard","doi":"10.1016/j.biosx.2025.100619","DOIUrl":"10.1016/j.biosx.2025.100619","url":null,"abstract":"<div><div>This study introduces a novel approach to cancer cell imaging by integrating microfluidic sensor technology with artificial intelligence (AI). We developed a custom microfluidic device with polydimethylsiloxane (PDMS) microchannels and integrated electrodes to capture electrical impedance data. The device was fabricated using photolithography, electron beam evaporation, and lift-off techniques. Instead of traditional imaging methods, electrical impedance signals were used to reconstruct cell images. A generative AI model with eight hidden layers processed 191 impedance values to accurately reconstruct the shapes of cancer cells and control beads. Our approach successfully reconstructed images of MDA-MB-231 breast cancer cells, HeLa cells, and beads, achieving 91 % accuracy on the test dataset. Validation using the Structural Similarity Index (SSI) and Mean Structural Similarity Index (MSSIM) produced scores of 0.97 for breast cancer cells and 0.93 for beads, confirming the high precision of this method. This label-free, impedance-based imaging offers a promising solution for cancer diagnostics by accurately reconstructing cell shapes and distinguishing cell types, particularly in point-of-care applications.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100619"},"PeriodicalIF":10.61,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced cardiovascular diagnostics using wearable ECG and bioimpedance monitoring with LightGBM classifier

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-26 DOI: 10.1016/j.biosx.2025.100617

Prince Jain , Ramji Gupta , Anand Joshi , Andrey Kuzmin

Cardiovascular diseases (CVDs) are the leading cause of death worldwide, necessitating advanced diagnostic and monitoring tools. Traditional methods of cardiac monitoring face challenges such as limited availability, high costs, and continuous physician oversight. Recent advancements in mobile health (mHealth) technologies, including wearable devices and mobile applications, offer promising solutions for continuous and real-time monitoring of vital signs such as ECG, bioimpedance, and physical activity. This study focuses on integrating these monitoring modalities to enhance the accuracy and reliability of cardiovascular diagnostics. Specifically, we explore the use of the MAX30001 device for precise ECG and bioimpedance measurements in wearable applications. Machine learning techniques, particularly LightGBM, are employed to classify cardiac conditions based on the collected data. The LightGBM classifier achieved a test set accuracy of 94.49 %, with precision, recall, and F1-scores above 0.95 for all classes. The model's performance was further validated through cross-validation (CV), yielding a 5-fold CV accuracy of 95.86 % and a 10-fold CV accuracy of 96.16 %. The ROC curve analysis showed excellent discriminatory ability with AUC values close to 1. These findings highlight the potential applications of advanced mHealth solutions in providing continuous, accurate, and real-time monitoring of cardiovascular health, which can lead to better patient management and outcomes through timely and informed interventions.

{"title":"Enhanced cardiovascular diagnostics using wearable ECG and bioimpedance monitoring with LightGBM classifier","authors":"Prince Jain , Ramji Gupta , Anand Joshi , Andrey Kuzmin","doi":"10.1016/j.biosx.2025.100617","DOIUrl":"10.1016/j.biosx.2025.100617","url":null,"abstract":"<div><div>Cardiovascular diseases (CVDs) are the leading cause of death worldwide, necessitating advanced diagnostic and monitoring tools. Traditional methods of cardiac monitoring face challenges such as limited availability, high costs, and continuous physician oversight. Recent advancements in mobile health (mHealth) technologies, including wearable devices and mobile applications, offer promising solutions for continuous and real-time monitoring of vital signs such as ECG, bioimpedance, and physical activity. This study focuses on integrating these monitoring modalities to enhance the accuracy and reliability of cardiovascular diagnostics. Specifically, we explore the use of the MAX30001 device for precise ECG and bioimpedance measurements in wearable applications. Machine learning techniques, particularly LightGBM, are employed to classify cardiac conditions based on the collected data. The LightGBM classifier achieved a test set accuracy of 94.49 %, with precision, recall, and F1-scores above 0.95 for all classes. The model's performance was further validated through cross-validation (CV), yielding a 5-fold CV accuracy of 95.86 % and a 10-fold CV accuracy of 96.16 %. The ROC curve analysis showed excellent discriminatory ability with AUC values close to 1. These findings highlight the potential applications of advanced mHealth solutions in providing continuous, accurate, and real-time monitoring of cardiovascular health, which can lead to better patient management and outcomes through timely and informed interventions.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100617"},"PeriodicalIF":10.61,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Ag@Au nanozyme-enhanced organic photoelectrochemical transistor for ultrasensitive ochratoxin A detection

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-21 DOI: 10.1016/j.biosx.2025.100612

Shusheng Wei, Yuchen Shen, zhanpeng Zhang, Juan Wang

Organic bioelectronic devices are developing as adaptable platforms for advanced biosensing applications, such as wearable sensors, neural interfaces and tissue engineering, due to their remarkable flexibility, mobility, ease of manufacture, and biocompatibility. The article presents a unique organic photoelectrochemical transistor (OPECT) sensor, combined with an Ag@Au nanozyme-mediated catalytic precipitation mechanism, creating an ultrasensitive detection platform for Ochratoxin A (OTA). The ZnO/ZnFe₂O₄ heterostructure is established as a novel gating module. The ZnFe₂O₄ layer may boost electrolyte interaction and light accessibility to the ZnO nanoarray, thereby modulating the response of the polymeric poly (3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel, which can be monitored through the channel current. In conjunction with aptamer sensing, the Ag@Au nanozyme, exhibiting peroxidase-mimicking activity, catalyzes the oxidation of 4-chloro-1-naphthol (4-CN), leading to the formation of an insoluble precipitate on the gate electrode surface, which diminishes the photocurrent and modifies the transistor response. The OPECT sensor demonstrates outstanding analytical capabilities for OTA, featuring a wide dynamic range from 10⁻⁵ ng/mL to 10 ng/mL and a detection limit of 0.0206 pg/mL. The advancement of this OPECT sensor offers potential for employing organic photoelectrochemical transistors as a high-performance platform for OTA detection.

{"title":"Innovative Ag@Au nanozyme-enhanced organic photoelectrochemical transistor for ultrasensitive ochratoxin A detection","authors":"Shusheng Wei, Yuchen Shen, zhanpeng Zhang, Juan Wang","doi":"10.1016/j.biosx.2025.100612","DOIUrl":"10.1016/j.biosx.2025.100612","url":null,"abstract":"<div><div>Organic bioelectronic devices are developing as adaptable platforms for advanced biosensing applications, such as wearable sensors, neural interfaces and tissue engineering, due to their remarkable flexibility, mobility, ease of manufacture, and biocompatibility. The article presents a unique organic photoelectrochemical transistor (OPECT) sensor, combined with an Ag@Au nanozyme-mediated catalytic precipitation mechanism, creating an ultrasensitive detection platform for Ochratoxin A (OTA). The ZnO/ZnFe<sub>2</sub>O<sub>4</sub> heterostructure is established as a novel gating module. The ZnFe<sub>2</sub>O<sub>4</sub> layer may boost electrolyte interaction and light accessibility to the ZnO nanoarray, thereby modulating the response of the polymeric poly (3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) channel, which can be monitored through the channel current. In conjunction with aptamer sensing, the Ag@Au nanozyme, exhibiting peroxidase-mimicking activity, catalyzes the oxidation of 4-chloro-1-naphthol (4-CN), leading to the formation of an insoluble precipitate on the gate electrode surface, which diminishes the photocurrent and modifies the transistor response. The OPECT sensor demonstrates outstanding analytical capabilities for OTA, featuring a wide dynamic range from 10<sup>−5</sup> ng/mL to 10 ng/mL and a detection limit of 0.0206 pg/mL. The advancement of this OPECT sensor offers potential for employing organic photoelectrochemical transistors as a high-performance platform for OTA detection.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100612"},"PeriodicalIF":10.61,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput and label-free screening of red blood cell stiffness: A study of sickle cell disease

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-20 DOI: 10.1016/j.biosx.2025.100616

Saurabh Kaushik , Arkabrata Mishra , Roshan Ross , Sweta Srivastava , Cecil R. Ross , Gautam V. Soni

Understanding the morphological and mechanical changes in cells are important for diagnostic and treatment methods in various diseases. In sickle cell disease (SCD), the mutated hemoglobin (HbS) aggregates inside the red blood cells (RBCs), making them rigid and, in extreme cases, sickle-shaped, resulting in anemia, episodes of pain, and multiple organ damage. Existing techniques are too costly and insensitive since the effect of the HbS gene (heterozygous and homozygous) is variable both in prevalence and clinical manifestations. In this work, we present a label-free, cost-effective, high-throughput electro-fluidic technique to study changes in the mechanical and morphological characteristics of RBCs. We validate our device by quantitatively comparing the mechanical properties of RBCs as a function of stiffness-altering drug (Latrunculin-A) with measurements using AFM. We demonstrate the on-site application of our system by screening SCD patients based on their RBC stiffness changes. The signatures of patient-specific heterogeneity in the RBC mechanical properties may help in monitoring clinical variability and identification of high-risk patients along with targeted therapies. The versatility of our measurements opens the whole cell stiffness as a preliminary screening biomarker in other haematological conditions, tumor cell identification, in veterinary sciences as well as in evaluating hydrogel technologies.

{"title":"High-throughput and label-free screening of red blood cell stiffness: A study of sickle cell disease","authors":"Saurabh Kaushik , Arkabrata Mishra , Roshan Ross , Sweta Srivastava , Cecil R. Ross , Gautam V. Soni","doi":"10.1016/j.biosx.2025.100616","DOIUrl":"10.1016/j.biosx.2025.100616","url":null,"abstract":"<div><div>Understanding the morphological and mechanical changes in cells are important for diagnostic and treatment methods in various diseases. In sickle cell disease (SCD), the mutated hemoglobin (HbS) aggregates inside the red blood cells (RBCs), making them rigid and, in extreme cases, sickle-shaped, resulting in anemia, episodes of pain, and multiple organ damage. Existing techniques are too costly and insensitive since the effect of the HbS gene (heterozygous and homozygous) is variable both in prevalence and clinical manifestations. In this work, we present a label-free, cost-effective, high-throughput electro-fluidic technique to study changes in the mechanical and morphological characteristics of RBCs. We validate our device by quantitatively comparing the mechanical properties of RBCs as a function of stiffness-altering drug (Latrunculin-A) with measurements using AFM. We demonstrate the on-site application of our system by screening SCD patients based on their RBC stiffness changes. The signatures of patient-specific heterogeneity in the RBC mechanical properties may help in monitoring clinical variability and identification of high-risk patients along with targeted therapies. The versatility of our measurements opens the whole cell stiffness as a preliminary screening biomarker in other haematological conditions, tumor cell identification, in veterinary sciences as well as in evaluating hydrogel technologies.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100616"},"PeriodicalIF":10.61,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

2D nanomaterials in biosensing: Synthesis, characterization, integration in biosensors and their applications

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-20 DOI: 10.1016/j.biosx.2025.100615

Desmond Lutomia , Renu Poria , Deepak Kala , Preeti Garg , Rupak Nagraik , Ankur Kaushal , Shagun Gupta , Deepak Kumar

Recent advances in the synthesis of functional nanomaterials and precisely engineered nanostructures have opened up new avenues for the fabrication of viable biosensors for field analysis. Two-dimensional (2D) nanomaterials provide unique hierarchical structures, high surface area, and layered configurations with multiple length scales and porosity, and the possibility to create functionalities for targeted recognition at their surface. In addition to providing extra features like structural color, ordered morphological features, and the capacity to detect and react to external stimuli, such hierarchical structures provide opportunities to tune the characteristics of materials. Combining these distinctive qualities of the various nanostructure types and using them as a foundation for bimolecular assemblies can yield biosensing platforms with enhanced robustness, sensitivity, and selectivity for the detection of a wide range of analytes, as well as targeted recognition and transduction properties that can have a positive impact on numerous fields. This review describes the classification, synthesis and characterization of 2D nanomaterials and their functionalization. In addition, the merits of the 2D nanomaterials and their applications in health, environmental monitoring and food safety and control are covered. The final part anticipates the advancement of 2D nanomaterials in biosensors, challenges and future directions of 2D nanomaterials in biosensors.

{"title":"2D nanomaterials in biosensing: Synthesis, characterization, integration in biosensors and their applications","authors":"Desmond Lutomia , Renu Poria , Deepak Kala , Preeti Garg , Rupak Nagraik , Ankur Kaushal , Shagun Gupta , Deepak Kumar","doi":"10.1016/j.biosx.2025.100615","DOIUrl":"10.1016/j.biosx.2025.100615","url":null,"abstract":"<div><div>Recent advances in the synthesis of functional nanomaterials and precisely engineered nanostructures have opened up new avenues for the fabrication of viable biosensors for field analysis. Two-dimensional (2D) nanomaterials provide unique hierarchical structures, high surface area, and layered configurations with multiple length scales and porosity, and the possibility to create functionalities for targeted recognition at their surface. In addition to providing extra features like structural color, ordered morphological features, and the capacity to detect and react to external stimuli, such hierarchical structures provide opportunities to tune the characteristics of materials. Combining these distinctive qualities of the various nanostructure types and using them as a foundation for bimolecular assemblies can yield biosensing platforms with enhanced robustness, sensitivity, and selectivity for the detection of a wide range of analytes, as well as targeted recognition and transduction properties that can have a positive impact on numerous fields. This review describes the classification, synthesis and characterization of 2D nanomaterials and their functionalization. In addition, the merits of the 2D nanomaterials and their applications in health, environmental monitoring and food safety and control are covered. The final part anticipates the advancement of 2D nanomaterials in biosensors, challenges and future directions of 2D nanomaterials in biosensors.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100615"},"PeriodicalIF":10.61,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An innovative and mass-sensitive quartz tuning fork (QTF) biosensor for GFAP detection: A novel approach for traumatic brain injury diagnosis

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-18 DOI: 10.1016/j.biosx.2025.100614

Burcu Özcan , İnci Uludağ Anıl , Mehmet Altay Ünal , Fikret Arı , Mustafa Kemal Sezgintürk , Sibel Ayşıl Özkan

The early diagnosis and management of traumatic brain injury (TBI) are dependent upon the early and precise detection of glial fibrillary acidic protein (GFAP). In this investigation, a novel biosensor based on quartz tuning forks (QTF) was introduced and functionalized with 11-mercaptoundecanoic acid (11-MUA). This biosensor is designed to facilitate the highly sensitive and selective detection of GFAP in human serum. In contrast to conventional neuroimaging methods, which are resource-intensive and frequently inaccessible in emergency situations, this innovative biosensor offers a portable, cost-effective, and efficient alternative for rapid GFAP measurement. The detection range of the system is 0.05 fg mL⁻¹ to 25 fg mL⁻¹. The Atomic Force Microscopy (AFM) was utilized to visualize the morphology of the QTF surface during the immobilization steps of the sensor. The developed biosensor presented advantages such as ability to determine GFAP concentrations at femtogram level, reproducibility and repeatability (standard deviation: ±0.0935966 Hz, and coefficient of variation: 7.91 %). This study highlights a significant progression in biosensing technology, providing an exceptionally sensitive and scalable platform for diagnosing neurological disorders, with potential uses in point-of-care environments.

{"title":"An innovative and mass-sensitive quartz tuning fork (QTF) biosensor for GFAP detection: A novel approach for traumatic brain injury diagnosis","authors":"Burcu Özcan , İnci Uludağ Anıl , Mehmet Altay Ünal , Fikret Arı , Mustafa Kemal Sezgintürk , Sibel Ayşıl Özkan","doi":"10.1016/j.biosx.2025.100614","DOIUrl":"10.1016/j.biosx.2025.100614","url":null,"abstract":"<div><div>The early diagnosis and management of traumatic brain injury (TBI) are dependent upon the early and precise detection of glial fibrillary acidic protein (GFAP). In this investigation, a novel biosensor based on quartz tuning forks (QTF) was introduced and functionalized with 11-mercaptoundecanoic acid (11-MUA). This biosensor is designed to facilitate the highly sensitive and selective detection of GFAP in human serum. In contrast to conventional neuroimaging methods, which are resource-intensive and frequently inaccessible in emergency situations, this innovative biosensor offers a portable, cost-effective, and efficient alternative for rapid GFAP measurement. The detection range of the system is 0.05 fg mL<sup>−1</sup> to 25 fg mL<sup>−1</sup>. The Atomic Force Microscopy (AFM) was utilized to visualize the morphology of the QTF surface during the immobilization steps of the sensor. The developed biosensor presented advantages such as ability to determine GFAP concentrations at femtogram level, reproducibility and repeatability (standard deviation: ±0.0935966 Hz, and coefficient of variation: 7.91 %). This study highlights a significant progression in biosensing technology, providing an exceptionally sensitive and scalable platform for diagnosing neurological disorders, with potential uses in point-of-care environments.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100614"},"PeriodicalIF":10.61,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time monitoring of ssDNA binding using a fiber optic LSPR microfluidic platform

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-18 DOI: 10.1016/j.biosx.2025.100613

Vivek Semwal , Asbjørn Meldgaard Moltke , Ole Bang , Jakob Janting

In this paper, we present the development of a localized surface plasmon resonance (LSPR) sensor for the detection of single-stranded DNA (ssDNA). The LSPR chip was fabricated using gold nanoparticles (AuNPs) with a diameter of 80 nm. It was integrated with a microfluidic chamber to ensure stable measurements. We employed data processing techniques to fit the absorbance curve and extract the resonance wavelength, significantly reducing noise and achieving a 100-fold improvement in signal quality. The fabricated LSPR chips demonstrated a bulk refractive index sensitivity of approximately 85–90 nm/RIU. This paper outlines a robust methodology for reliable LSPR measurements based on cheap and readily accessible instruments. We have shown successfully real-time binding between Poly(T₂₀) and Poly(A₂₀), sensitive down to a concentration of 2 nM, while maintaining signal fluctuations 10 times lower than the shift in resonance wavelength without using any complex signal amplification technique. The sensor exhibits a limit of detection (LOD) of 0.75 nM. The proposed method shows potential for high-sensitivity and reliable real-time detection of smaller biomolecules, environmental pollutants, foodborne pathogens, toxins, and disease biomarkers.

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引用次数: 0

Hot or not: Quantifying isothiocyanates in plants, soil, and other media

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X

Pub Date : 2025-03-15 DOI: 10.1016/j.biosx.2025.100599

Octavia Hogaboam , Viola A. Manning , Catherine L. Reardon , Kristin M. Trippe

Isothiocyanates (ITCs) are bioactive compounds produced by plants in the Brassicales that serve as natural defense mechanisms against pests and pathogens, and provide sharp, hot, and pungent flavors to plants like wasabi, mustards, and horseradish. In agricultural settings, natural and synthetic ITCs are used to biofumigate soils prior to planting; however, because residual ITCs can inhibit germination or plant growth, (i.e. soils are “hot”), caution is necessary when fields are replanted. Current methodologies that measure ITCs are labor intensive and require expensive instrumentation. Therefore, there is a critical need for rapid, reliable, and inexpensive methods that detect ITCs in a variety of plant and soil matrices. This study describes the development and validation of the SaxAPIL biosensor, which uses an ITC-responsive promoter to drive expression of a bioluminescent reporter in Pseudomonas fluorescens SBW25 to quantify ITCs. Our results indicate that SaxAPIL can be used in a high throughput microplate-based assay to detect ITCs in a dose-dependent manner in solutions, plant- and seed meal-derived extracts, and soils amended with green manure or seed meals. Our results clearly demonstrate that SaxAPIL is a robust biosensor for the detection and quantification of aliphatic ITCs in plants and soil. The methodology presented here may be adapted to provide more efficient and less expensive methods to measure ITCs in industrial, health, and life science applications.

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引用次数: 0