Expression and clinical diagnostic value of CCHE1 in breast cancer.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-03-12 DOI:10.1093/mutage/gead038

Feng Zhao, Wenjuan Zhou, Ran Xin, Xin Miao

{"title":"Expression and clinical diagnostic value of CCHE1 in breast cancer.","authors":"Feng Zhao, Wenjuan Zhou, Ran Xin, Xin Miao","doi":"10.1093/mutage/gead038","DOIUrl":null,"url":null,"abstract":"Objective: Breast cancer is a malignant tumor in the epithelial tissue of the breast gland. This study aimed to unveil the expression and clinical diagnostic value of lncRNA cervical cancer high-expressed 1 (CCHE1) in breast cancer.Methods: CCHE1 expression in breast cancer tissues was evaluated by RT-qPCR. The relationship between the CCHE1 expression and clinicopathological features of breast cancer was analyzed with the chi-square test, and the survival of breast cancer patients was evaluated with the Kaplan-Meier method. The diagnostic value of CCHE1 expression for breast cancer was evaluated by using the receiver operating characteristics (ROC) curve. Breast cancer cell lines (SKBR3, T47D, BT474, and MCF-7) were cultured for detecting CCHE1 expression in the cells. MCF-7 cells were selected for the subsequent experiments, and the small interfering RNA of CCHE1 (si-CCHE1) and CCHE1 overexpression vector (pcDNA-CCHE1) were transfected into MCF-7 cells. The proliferation, migration, and invasive ability were assessed by CCK-8 and Transwell assays. The influence of CCHE1 on the growth of tumors was validated by nude mice xenograft assay.Results: CCHE1 was up-regulated in breast cancer tissues and breast cancer cells. The high expression level of CCHE1 in cancer tissues of breast cancer patients was correlated with larger tumor size, advanced TNM stage, Ki-67 status, and lymph node metastasis. The area under the ROC curve for CCHE1 in the diagnosis of breast cancer was 0.983 (95% CI: 0.966-1.000), with a sensitivity of 95.00% and a specificity of 91.70%. The 5-year survival rate was higher in patients with low CCHE1 expression than those with high CCHE1 expression. Furthermore, restrained CCHE1 impeded proliferation, invasion, and migration of MCF-7 cells, as well as tumor growth in mice.Conclusion: Our study highlights that elevated expression of CCHE1 in breast cancer tissues, which is closely related to clinicopathologic features, has some clinical value in the diagnosis of the disease.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/mutage/gead038","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: Breast cancer is a malignant tumor in the epithelial tissue of the breast gland. This study aimed to unveil the expression and clinical diagnostic value of lncRNA cervical cancer high-expressed 1 (CCHE1) in breast cancer.

Methods: CCHE1 expression in breast cancer tissues was evaluated by RT-qPCR. The relationship between the CCHE1 expression and clinicopathological features of breast cancer was analyzed with the chi-square test, and the survival of breast cancer patients was evaluated with the Kaplan-Meier method. The diagnostic value of CCHE1 expression for breast cancer was evaluated by using the receiver operating characteristics (ROC) curve. Breast cancer cell lines (SKBR3, T47D, BT474, and MCF-7) were cultured for detecting CCHE1 expression in the cells. MCF-7 cells were selected for the subsequent experiments, and the small interfering RNA of CCHE1 (si-CCHE1) and CCHE1 overexpression vector (pcDNA-CCHE1) were transfected into MCF-7 cells. The proliferation, migration, and invasive ability were assessed by CCK-8 and Transwell assays. The influence of CCHE1 on the growth of tumors was validated by nude mice xenograft assay.

Results: CCHE1 was up-regulated in breast cancer tissues and breast cancer cells. The high expression level of CCHE1 in cancer tissues of breast cancer patients was correlated with larger tumor size, advanced TNM stage, Ki-67 status, and lymph node metastasis. The area under the ROC curve for CCHE1 in the diagnosis of breast cancer was 0.983 (95% CI: 0.966-1.000), with a sensitivity of 95.00% and a specificity of 91.70%. The 5-year survival rate was higher in patients with low CCHE1 expression than those with high CCHE1 expression. Furthermore, restrained CCHE1 impeded proliferation, invasion, and migration of MCF-7 cells, as well as tumor growth in mice.

Conclusion: Our study highlights that elevated expression of CCHE1 in breast cancer tissues, which is closely related to clinicopathologic features, has some clinical value in the diagnosis of the disease.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

CCHE1 在乳腺癌中的表达和临床诊断价值。

目的：乳腺癌是乳腺上皮组织的恶性肿瘤。本研究旨在揭示lncRNA宫颈癌高表达1（CCHE1）在乳腺癌中的表达及临床诊断价值：方法：通过 RT-qPCR 评估 CCHE1 在乳腺癌组织中的表达。方法：通过 RT-qPCR 评估 CCHE1 在乳腺癌组织中的表达，采用卡方检验分析 CCHE1 表达与乳腺癌临床病理特征之间的关系，并采用 Kaplan-Meier 法评估乳腺癌患者的生存率。CCHE1表达对乳腺癌的诊断价值采用接收者操作特征曲线（ROC）进行评估。培养乳腺癌细胞系（SKBR3、T47D、BT474和MCF-7）以检测细胞中CCHE1的表达。选择 MCF-7 细胞进行后续实验，并将 CCHE1 小干扰 RNA（si-CCHE1）和 CCHE1 过表达载体（pcDNA-CCHE1）转染至 MCF-7 细胞。CCK-8和Transwell试验评估了细胞的增殖、迁移和侵袭能力。裸鼠异种移植试验验证了 CCHE1 对肿瘤生长的影响：结果：CCHE1在乳腺癌组织和乳腺癌细胞中上调。CCHE1在乳腺癌患者癌组织中的高表达水平与肿瘤体积增大、TNM分期晚期、Ki-67状态和淋巴结转移相关。CCHE1 诊断乳腺癌的 ROC 曲线下面积为 0.983（95% CI：0.966-1.000），灵敏度为 95.00%，特异度为 91.70%。CCHE1低表达患者的5年生存率高于CCHE1高表达患者。此外，抑制 CCHE1 会阻碍 MCF-7 细胞的增殖、侵袭和迁移以及小鼠的肿瘤生长：结论：我们的研究表明，CCHE1在乳腺癌组织中的高表达与临床病理特征密切相关，在疾病诊断中具有一定的临床价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊