Characterization of Meloidogyne enterolobii intercepted from baobab (Adansonia digitata L.) seedlings from Thailand during Japanese import plant quarantine inspection.

IF 1.1 4区生物学 Q4 PARASITOLOGY Helminthologia Pub Date : 2023-12-26 eCollection Date: 2023-09-01 DOI:10.2478/helm-2023-0027

S Sekimoto, T Inaba

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Abstract

In April 2019, baobab (Adansonia digitata L.) seedlings from Thailand, exhibiting galls on the roots, were intercepted during an import plant quarantine inspection at Chubu Centrair International Airport, Japan. Root-knot nematodes (RKNs) were extracted from the galled roots of baobab seedlings and identified by morphological, morphometrical and molecular methods as the guava root-knot nematode, Meloidogyne enterolobii Yang & Eisenback. The morphology and morphometrics of the intercepted population were similar to those of the original and subsequent descriptions of M. enterolobii. The sequences of D2-D3 of 28S rRNA, mtDNA intergenic COII-16S rRNA and COI genes obtained in this study matched well (99-100% similarity) with each of the gene sequences of M. enterolobii deposited in GenBank. Phylogenetic analysis of these genes revealed that the intercepted population clustered with M. enterolobii and clearly differed from other RKN species. To the best of our knowledge, this is the first report of M. enterolobii from baobab.

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日本进口植物检疫检验期间从泰国猴面包树（Adansonia digitata L.）幼苗中截获的肠道黑僵菌的特征。

2019年4月，日本中部Centrair国际机场在一次进口植物检疫检查中截获了来自泰国的猴面包树（Adansonia digitata L.）幼苗，其根部出现虫瘿。从猴面包树幼苗有虫瘿的根部提取了根结线虫（RKNs），并通过形态学、形态计量学和分子学方法鉴定为番石榴根结线虫 Meloidogyne enterolobii Yang & Eisenback。截获种群的形态和形态计量学特征与最初和后来对 M. enterolobii 的描述相似。本研究获得的 28S rRNA、mtDNA 基因间 COII-16S rRNA 和 COI 基因的 D2-D3 序列与 GenBank 中保存的 M. enterolobii 各基因序列吻合良好（相似度为 99-100%）。这些基因的系统进化分析表明，截获的种群与 M. enterolobii 聚类，与其他 RKN 物种明显不同。据我们所知，这是第一份关于猴面包树肠杆菌的报告。

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来源期刊

Helminthologia 生物-动物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Helminthologia (HELMIN), published continuously since 1959, is the only journal in Europe that encompasses the individual and collaborative efforts of scientists working on a different topics of human, veterinary and plant helminthology. The journal responsibility is to enrich the theoretical and practical knowledge in very specific areas and thus contribute to the advancements in human and veterinary medicine and agronomy. Taking the advantage of comprehensive and multidisciplinary approaches journal still maintains its original spirit and is principal source of fresh scientific information regarding helminths, endoparasites and plant parasites. Addressing the most up-to date topics journal gained rightful and exceptional place next to the other high-quality scientific journals publishing in its field.