Helminthologia最新文献

Infections with hookworms (Necator americanus and Ancylostoma duodenale) remain a major public health problem in low- and middle-income countries. However, the information about the distribution of each species is inaccurate in many countries since their traditional diagnosis is based only on the identification of eggs in stool under a microscope. We aimed to identify the prevalence of hookworm species using morphological stools to identify L3 larvae to gain insights into the distribution of both species in five regions of Cameroon. Samples were collected from schoolchildren in five regions and 34 subdivisions of Cameroon and examined using the Kato-Katz method. We randomly selected a total of 157 samples among hookworm's positive stool samples. They were cultured using the Harada-Mori test-tube technique. The morphological identification of a total of 8063 isolated hookworm filariform larvae L3 was conducted following established criteria. The sensitivity rate to the Harada-Mori technique was 58 %. Among the 8063 L3 larvae identified during this study, 230 (2.95 %) of L3 larvae were identified as A. duodenale, and 7833 (97.15 %) of L3 larvae were identified as N. americanus. A. duodenale was observed only in the Mouanko subdivision in the Littoral region. The complementary use of the Kato Katz and the Harada-Mori culture techniques to screen hookworm infections contributes to the differentiation of N. americanus and A. duodenale as the two hookworm species in Cameroon. An extended molecular study in the localities where only N. americanus has been identified is necessary to reach more conclusions on the distribution of hookworm species in Cameroon.

The development of turkey farming is significantly restrained by parasitic diseases, which have become widespread and cause significant economic losses to specialized- and homestead farms. This study aimed to determine the prevalence of endoparasites in turkeys of different age groups under different maintenance systems and the nature of patho-anatomical changes depending on the course of the disease. A total of 1,869 samples of fecal from turkeys were studied. From turkeys kept in poultry houses, 925 fecal samples and 944 fecal samples from free-range turkeys were examined. In turkeys that were kept in a poultry house, the total infection with endoparasites was 59.8 %, while in free range, it was 63.3 %. The most common in turkeys 30-60 days old from poultry houses are Histomonas meleagridis (21.5 %), Eimeria spp. (11.5 %) and Cryptosporidium baileyi (15.5 %) and in free-range turkeys - Histomonas meleagridis (15.6 %), Eimeria spp. (7.2 %) and Cryptosporidium baileyi (10.8 %). Cryptosporidium baileyi was not observed in turkeys 360 days old or older. Tetratrichomonas gallinarum was most frequently recorded in turkeys 90 - 120 days old from poultry houses (3.8 %) and turkeys 150 - 180 days old in free range (3.7 %). Ascaridia dissimilis is most common in free-range turkeys 90 - 120 days old (11.8 %) and turkeys 150 - 180 days old from poultry houses (9.5 %). Heterakis gallinarum was primarily recorded in turkeys 150 - 180 days old under both systems of keeping with a value range from 16.2 to 17.2 %. Infestation of 150 - 180-day-old free-range turkeys by cestodes Davainea meleagridis and Raillietina echinobothrida was 15.0 % and 12.9 %, respectively. Thus, the diversity and prevalence of gastrointestinal tract invasions depend on the age and housing system of the poultry.

A 25-year-old intensive monoculture of cucumber (Cucumis sativus 'Monolit F1') has been chosen for monitoring the effect of continuous fertilization, drip irrigation and various soil disinfection on free-living nematodes in soil. Densities, relative abundance of nematode genera, trophic groups and c-p classes, in addition, numerous nematological indices were determined besides certain soil properties. Concerning the nematological indices, all the values proved a stressed, depleted and unstable environment with degraded food web condition. The exaggerated application of fertilizers through irrigation caused high salinity values (0.62 %) which is much higher than the indicated toxicity threshold (0.4 %) for cultivated plants. The number of individuals of the bacterivore Acrobeloides and Cuticularia genera, which have a very high stress tolerance, was outstanding in the area. Besides juveniles, the appearance of Meloidogyne incognita males was also recorded which could lead to a change in the genetic composition of the population to adapt to environmental stressors. All these changes may result from salt stress indicated by intensive drip irrigation and the use of artificial fertilizers.

Background: Infections with intestinal parasites are the major cause of infectious disease globally and have been described as a public health issue in developing countries.

Objective: This study aimed to determine prevalence of intestinal parasitic infections and their associated factors among the population of Erbil province in Iraq over the period 2011-2021.

Methods: The results of 614455 stool examinations of all public health facilities of the province were collected from the register in the directorate of preventive health affairs and the data were analyzed.

Result: The overall prevalence of intestinal parasitic infections was 4.24 % in the province. The total number of reported cases of intestinal parasites decreased from 4352 for 2011 to 1728 for 2018 and then increased to 2014 cases for 2021. Nearly half of the intestinal parasitic infections were detected among age group of 15 to 44 years. The prevalence of intestinal parasitic infections was higher in males (65.73 %) than females (34.27 %). The prevalence of intestinal parasitic infections was 9 0.47 % for protozoan infections, while the rate of helminth infection was lower (9 .53 %). Entamoeba histolytica/dispar were the most common parasite (60.92 %) followed by Giardia lamblia (29.54 %) and Enterobius vermicularis (8.56 %). Prevalence of infection with intestinal parasites was lowest in March and highest in February.

Conclusion: In spite of that intestinal parasitic infection rates appear to be decreasing, intestinal parasitic infection remains an important health problem in the province. Therefore, there is still a need for prevention efforts in the community.

The brown marmorated stink bug (Halyomorpha halys), a native of Asia, has become an invasive pest in North America and Europe. Given the severity of H. halys and the need for better and environmentally-friendly control methods for this pest, we evaluated the virulence four entomopathogenic nematode species (Heterorhabditis indica, Oscheius myriophilus, Steinernema eapokense and Steinernema pakistanense) that occur naturally in soils in southern Việt Nam and compared them with that of a commercially available strain of S. carpocapsae. We report for the first time the pathogenicity of Oscheius myriophilus towards BMSB. The commercial strain of S. carpocapsae was most virulent against BMSB. However, the strain of S. pakistanense isolated from soil in southern Việt Nam has potential for the biological control of this pest. To achieve this potential, further work is needed including evaluation in the field and with the formulation of the nematode inoculum.

The search for hitherto undiscovered larvae of Anisakis sp. from marine habitat in the Indian sub-continent yielded A. typica (Dujardin) larvae hitherto unconfirmed. The present study is the maiden attempt to report 3^rd stage larvae of A. typica from the reef-associated Sillago sihama in Arabian Sea off the coast of Goa, which has been identified recently as reef-populated area within the maritime boundary of India. The morphometry of 3^rd stage larvae has been presented with a record of molecular characterization. In the context of current study, the natural prevalence of A. typica larvae in marine piscine hosts of Arabian Sea in India was 6.84 % and of co-occurring Rotundocollarette capoori (Yadav, Kapoor and Malhotra) in the same fish was 13.65 %. The roundworms were confirmed to be A. typica by application of the molecular and genetic characterization based on ITS1, ITS2 and 18S rDNA sequence analysis. The infestation of reef-associated fishes in this study by anisakid worms study provided an opportunity to explore mechanism of ecological associations of coral reefs with parasitization in future.

The recent publication entitled "Possible zoonotic implications of the discovery of the advanced third stage larva of Gnathostoma turgidum (Spirurida: Gnathostomatidae) in a Mexican fish species" by Mosqueda-Cabrera et al., appeared in Helminthologia, 2023; 60(1): 112-116. was extremely interesting and informative showing potential zoonotic natue of G. turgidum. However, discovery of only one larva from one out of 17 fish rather indicate that this fish is an accidental host of G. turgidum. Whether this and other fish act as the second intermediate host in the life cycle of G. turgidum requires more field survey using both compression and digestion methods. Also, many biological events of G. turgidum such as intrahepatic maturation in the final hosts and the seasonal changes with self cure are remained unsolved. More extensive survey on intermediate and paratenic hosts for G. turgidum and biological research on unique features of G. turgidum is necessary.