Immediate implantation of ultrafine fiber slow-release system based on cell electrospinning to induce osteogenesis of mesenchymal stem cells

IF 5.6 1区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Regenerative Biomaterials Pub Date : 2023-12-24 DOI:10.1093/rb/rbad113

Tao Lu, Long Yang, Zhuo-yang Li, Yin Liu, Shun-en Xu, Chuan Ye

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Abstract

This study presents the development and evaluation of a P34HB ultrafine fiber slow-release system for in vivo osteogenic induction of Human Umbilical Cord Mesenchymal Stem Cells (HUCMSCs). Utilizing dual-nozzle and cell electrospinning techniques, the system encapsulates L-ascorbic acid-2-phosphate magnesium (ASP), β-glycerophosphate sodium (GP), and dexamethasone (DEX) within the fibers, ensuring sustained osteogenic differentiation. The scaffold's morphology, characterization, hydrophilicity, mechanical properties, and cellular behavior were examined. Immediate subcutaneous implantation in rabbits was conducted to observe its ectopic osteogenic induction effect. Successfully fabricated P34HB ultrafine fiber slow-release system. Characterization confirmed the uniform distribution of HUCMSCs and inducing components within the scaffold, with no chemical reactions affecting the active components. In vitro tests showcased a prolonged release of DEX and ASP, while biocompatibility assays highlighted the scaffold's suitability for cellular growth. Alizarin Red, Type I Collagen, and Osteopontin (OPN) staining verified the scaffold's potent osteogenic induction effect on HUCMSCs. Notably, immediate implantation into New Zealand white rabbits led to significant new bone formation within 8 weeks. These findings underscore the system's potential for immediate in vivo implantation without prior in vitro induction, marking a promising advancement in bone tissue engineering.

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基于细胞电纺丝的超细纤维缓释系统即时植入，诱导间充质干细胞成骨

本研究介绍了用于体内诱导人脐间质干细胞（HUCMSCs）成骨的 P34HB 超细纤维缓释系统的开发和评估。该系统利用双喷嘴和细胞电纺技术，在纤维中封装了L-抗坏血酸-2-磷酸镁（ASP）、β-甘油磷酸钠（GP）和地塞米松（DEX），确保了持续的成骨分化。研究人员对支架的形态、特性、亲水性、机械性能和细胞行为进行了检测。在兔子皮下立即植入，观察其异位成骨诱导效果。成功制备了 P34HB 超细纤维缓释系统。表征证实，HUCMSCs 和诱导成分在支架内分布均匀，活性成分未受化学反应影响。体外测试表明，DEX 和 ASP 的释放时间延长，而生物相容性测试则强调了该支架对细胞生长的适用性。茜素红（Alizarin Red）、I 型胶原和骨生成素（Osteopontin，OPN）染色验证了该支架对 HUCMSCs 的强效成骨诱导作用。值得注意的是，立即植入新西兰白兔体内可在 8 周内形成大量新骨。这些发现强调了该系统无需事先进行体外诱导即可立即植入体内的潜力，标志着骨组织工程学取得了令人鼓舞的进展。

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来源期刊

Regenerative Biomaterials Materials Science-Biomaterials

CiteScore

7.90

自引率

16.40%

发文量

审稿时长

10 weeks

期刊介绍： Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.