A model of pregnancy-associated malaria for inducing adverse pregnancy outcomes in ICR mouse

IF 1.4 4区医学 Q3 PARASITOLOGY Experimental parasitology Pub Date : 2023-12-27 DOI:10.1016/j.exppara.2023.108686

Yingying Zhang , Zhiming Liang , Haoyu Xing , Chuyi Yu , Jianming Liang , Qin Xu , Jianping Song , Zhouqing He

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Abstract

Background

Based on understanding of placental pathological features and safe medication in pregnancy-associated malaria (PAM), establishment of a stable pregnant mouse infection model with Plasmodium was urgently needed.

Methods

ICR mice with vaginal plugs detected were randomly divided into post-pregnancy infection (Malaria⁺) and uninfected pregnancy (Malaria⁻) cohorts. Age-matched mice that had not been mated were infected as pre-pregnancy infection group (Virgin control), which were subsequently mated with ICR males. All mice were inoculated with 1 × 10⁶ Plasmodium berghei ANKA-infected RBCs by intraperitoneal injection, and the same amount of saline was given to Malaria⁻ group. We recorded the incidence of adverse pregnancy outcomes and the amounts of offspring in each group.

Results

The Virgin group mice were unable to conceive normally, and vaginal bleeding, abortion, or stillbirth appeared in the Malaria⁺ group. The incidence of adverse pregnancy outcomes was extremely high and statistically significant compared with the control (Malaria⁻) group (P < 0.05), of which placenta exhibited pathological features associated with human gestational malaria.

Conclusions

The intraperitoneal injection of 1 × 10⁶ Plasmodium berghei ANKA-infected RBCs could establish a model of pregnancy-associated malaria in ICR mouse.

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诱导 ICR 小鼠不良妊娠结局的妊娠相关疟疾模型

背景基于对妊娠相关疟疾（PAM）胎盘病理特征和安全用药的了解，迫切需要建立稳定的妊娠小鼠疟原虫感染模型。未交配的年龄匹配小鼠作为孕前感染组（处女对照组），随后与ICR雄性小鼠交配。所有小鼠均腹腔注射 1×106 感染了伯格希氏疟原虫 ANKA 的红细胞，疟疾组则注射等量的生理盐水。结果处女组小鼠不能正常受孕，疟疾+组出现阴道出血、流产或死胎。结果处女组小鼠不能正常受孕，疟疾+组出现阴道出血、流产或死胎，不良妊娠结局发生率极高，与对照（疟疾-）组相比有统计学意义（P < 0.05），其中胎盘表现出与人类妊娠疟疾相关的病理特征。

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来源期刊

Experimental parasitology 医学-寄生虫学

CiteScore

3.10

自引率

4.80%

发文量

160

审稿时长

3 months

期刊介绍： Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.