Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2023-12-01 DOI:10.18502/ijm.v15i6.14139

Ibiam Francis Amadi, Obasikene Catherine Nchedo, Ariom Thaddaeus Obaji, Monday Agbonifo, Egwu Eze, Iroha Chidinma Stacy, Moses Ikechukwu Benjamin, Iroha Ifeanyichukwu Romanus

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Abstract

Background and objectives: AmpC-producing Gram-negative bacterial (GNB) pathogens are distributed worldwide, especially in clinical settings. This study aimed to determine the antibiogram and the type of AmpC-β-lactamase gene harboured by GNB pathogens implicated in chronic suppurative otitis media (CSOM) cases.

Materials and methods: Ear swab samples (300) collected from patients with active CSOM were analysed using standard microbiological techniques. Phenotypic and molecular detection of AmpC β-lactamase production was done by cefoxitin/cloxacillin double-disk synergy test and PCR respectively. Antibiogram was determined by disk diffusion technique.

Results: Among the GNB pathogens isolated from CSOM patients, P. aeruginosa was the most predominant (36.3%); followed by K. pneumoniae (22.3%), and E. coli (13.7%). Patients with active CSOM showed increased bacteria isolation rate from bilateral ear discharges than unilateral ear discharges. E. coli and P. aeruginosa were more prevalent among patients with duration of discharge >2 weeks; recording 9.0% and 20.3% respectively. AmpC β-lactamase producers accounted for 14.0%; they were highly resistant (60%-100%) to cephalosporins, trimethoprim-sulfamethoxazole, ofloxacin, amoxicillin, and tetracycline, but very susceptible (70.4%-100%) to ciprofloxacin, imipenem, and amikacin. Multiple antibiotic resistance indices of isolates ranged from 0.7-0.8. FOX-AmpC-β-lactamase gene was detected in 3.9% of the isolates.

Conclusion: The detection of AmpC β-lactamase-producing multidrug-resistant GNB pathogens harbouring FOX-AmpC-β-lactamase gene among patients with CSOM infections in our study is a serious public health problem which needs urgent intervention.

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从尼日利亚慢性化脓性中耳炎患者中分离出的产 AmpC-β-内酰胺酶病原体的 FOX-AmpC-β-内酰胺酶基因检测和抗生素图谱。

背景和目的：产AmpC的革兰氏阴性细菌（GNB）病原体分布于世界各地，尤其是在临床环境中。本研究旨在确定慢性化脓性中耳炎（CSOM）病例中的革兰氏阴性菌病原体所携带的抗生素图谱和 AmpC-β- 内酰胺酶基因类型：采用标准微生物学技术分析从活动性 CSOM 患者处采集的耳拭子样本（300 份）。通过头孢西丁/氯唑西林双盘协同试验和聚合酶链式反应分别对 AmpC β-内酰胺酶的产生进行表型和分子检测。抗菌谱通过盘扩散技术进行测定：在从 CSOM 患者体内分离出的 GNB 病原菌中，铜绿假单胞菌占多数（36.3%），其次是肺炎双球菌（22.3%）和大肠杆菌（13.7%）。与单侧耳分泌物相比，活动性 CSOM 患者双侧耳分泌物的细菌分离率更高。大肠杆菌和铜绿假单胞菌在出院时间超过两周的患者中更为常见，分别占 9.0% 和 20.3%。AmpC β-内酰胺酶产生者占 14.0%；它们对头孢菌素、三甲双氨-磺胺甲噁唑、氧氟沙星、阿莫西林和四环素高度耐药（60%-100%），但对环丙沙星、亚胺培南和阿米卡星非常敏感（70.4%-100%）。分离物对多种抗生素的耐药性指数在 0.7-0.8 之间。3.9%的分离株检测到 FOX-AmpC-β-内酰胺酶基因：结论：在我们的研究中，CSOM感染患者中检出了携带FOX-AmpC-β-内酰胺酶基因的产AmpC β-内酰胺酶多重耐药GNB病原体，这是一个严重的公共卫生问题，亟需干预。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.