Performance evaluation of the Sysmex XN-1000V in side-by-side comparison with the Siemens ADVIA 120 and manual methods for healthy CD Sprague–Dawley rats and CD-1 mice

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES Veterinary clinical pathology Pub Date : 2024-01-02 DOI:10.1111/vcp.13314

Jill M. Schroeder, Hsiu-Yung C. Lee, Albert E. Schultze

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Abstract

Background

The Sysmex XN-1000V automated hematology analyzer with multispecies software was released in June 2017 for use in research laboratories. Laser light, impedance, fluorescent staining, and fluorescent flow cytometry are used to analyze whole blood for CBC, reticulocyte counts, and WBC counts, including a 5-part differential leukocyte analysis.

Objectives

A side-by-side comparison of the Sysmex XN-1000V with the Siemens ADVIA 120 in analyzing blood from healthy mice and rats will provide insight into the performance of the new analyzer and its capabilities for use in drug development studies. Method correlation analyses on normal mouse and rat hematology data collected with both analyzers and manual reference methods will help determine the reliability of the data produced using the Sysmex XN-1000V analyzer.

Methods

Whole blood samples collected in K₂EDTA from healthy CD-1 mice and CD Sprague–Dawley rats were analyzed in parallel with the XN-1000V and ADVIA 120 analyzers. Male and female mice, approximately 6–9 weeks old, and male and female rats, approximately 7–9 weeks old, were included in this study. Manual reference methods for WBC differential leukocyte analysis and packed cell volume (PCV) measurements were also performed. EP Evaluator version 11.2 (Data Innovations LLC, South Burlington, VT, USA) was used for method comparison statistical analysis.

Results

Most hematologic parameters for naïve mice and rats achieved correlation in the fair to excellent range, with the majority showing very good to excellent correlation with low biases (<11.0%) for cohorts analyzed separately and when cohort data were combined.

Conclusions

The Sysmex XN-1000V Hematology Analyzer provided comparable results to those obtained from the Siemens ADVIA 120. We found the Sysmex XN-1000V Hematology Analyzer to be acceptable for use in drug development studies for rats and mice.

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Sysmex XN-1000V 与西门子 ADVIA 120 和手动方法并排比较，对健康 CD Sprague-Dawley 大鼠和 CD-1 小鼠进行性能评估。

背景介绍Sysmex XN-1000V 自动血液分析仪与多物种软件于 2017 年 6 月发布，供研究实验室使用。激光、阻抗、荧光染色和荧光流式细胞仪用于分析全血的 CBC、网织红细胞计数和白细胞计数，包括 5 部分白细胞差异分析：Sysmex XN-1000V 与西门子 ADVIA 120 在分析健康小鼠和大鼠血液时的并排比较将有助于深入了解新分析仪的性能及其在药物开发研究中的应用能力。对使用这两种分析仪和手动参考方法收集的正常小鼠和大鼠血液学数据进行方法相关性分析，将有助于确定使用 Sysmex XN-1000V 分析仪生成的数据的可靠性：用 XN-1000V 和 ADVIA 120 分析仪同时分析从健康的 CD-1 小鼠和 CD Sprague-Dawley 大鼠采集的 K2 EDTA 全血样本。研究对象包括约 6-9 周大的雌雄小鼠和约 7-9 周大的雌雄大鼠。还进行了白细胞差异分析和包装细胞体积（PCV）测量的手动参考方法。EP Evaluator 11.2 版（Data Innovations LLC，South Burlington，VT，USA）用于方法比较统计分析：结果：新手小鼠和大鼠的大多数血液学参数的相关性在一般到优秀之间，大多数参数的相关性在非常好到优秀之间，偏差较小（结论：Sysmex XN-1 是一种新型的血液学分析仪：Sysmex XN-1000V 血液分析仪提供的结果与西门子 ADVIA 120 的结果相当。我们认为 Sysmex XN-1000V 血液分析仪可用于大鼠和小鼠的药物开发研究。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.