首页 > 最新文献

Veterinary clinical pathology最新文献

英文 中文
Evaluation of the analytical performance of a point-of-care analyzer for the measurement of feline serum thyroxine concentration in comparison with a chemiluminescence enzyme immunoassay.
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-02-10 DOI: 10.1111/vcp.13416
Agnes C Gläsel, Kristina Weiler, Alexander Pankraz, Natali Bauer

Background: Total thyroxine (TT4) measurement is used to assess thyroid status in cats.

Objectives: The aim of the prospective study was to evaluate the analytical performance of the point-of-care analyzer (POCA) Immuno AU10V using the v-T4 test kit for feline TT4 measurement. Additionally, method comparison with a benchtop analyzer (IMMULITE 2000) was done.

Methods: Validation included linearity, inter- and intra-assay precision, precision near the lower limit of quantification (LloQ), and interference testing for hemoglobin, lipid, and bilirubin. Correlation and bias were assessed.

Results: Linearity was given within the dynamic range. Coefficients of variation (CV) were ≤4% near the LloQ as well as for intra-and inter-assay precision. No interference was observed for lipid and bilirubin, while hemoglobin caused a negative bias of 28%. Method comparison included 74 samples within three TT4 concentration ranges (0.5-3.7, >3.7-5.13, >5.13-8 μg/dL). Correlation between POCA and reference method was excellent (rs = 0.95) with a slight proportional bias of 4.5%. TEobs was between 7.0% and 9.8%. Despite substantial agreement, discordant results on thyroid status occurred in 15% of samples.

Conclusions: The analytical performance of the POCA was excellent, as was its correlation with the reference method. Except for the interferent effect of hemoglobin, the TEobs was a for all analyses. Analysis of severely hemolytic samples is not advised. However, the relatively small dynamic range of the POCA precludes quantitative analysis of samples with TT4 >8 μg/dL, and de novo reference intervals need to be established.

背景:总甲状腺素(TT4)测量用于评估猫的甲状腺状态:总甲状腺素(TT4)测定用于评估猫的甲状腺状态:这项前瞻性研究的目的是评估使用v-T4检测试剂盒测量猫TT4的护理点分析仪(POCA)Immuno AU10V的分析性能。此外,还与台式分析仪(IMMULITE 2000)进行了方法比较:验证包括线性、测定间和测定内精密度、接近定量下限(LloQ)的精密度以及血红蛋白、血脂和胆红素的干扰测试。对相关性和偏差进行了评估:结果:在动态范围内呈线性。在 LloQ 附近的变异系数(CV)小于 4%,测定内和测定间的精密度也小于 4%。血脂和胆红素未发现干扰,而血红蛋白的负偏差为 28%。方法比较包括三个 TT4 浓度范围(0.5-3.7、>3.7-5.13、>5.13-8 μg/dL)内的 74 份样本。POCA 与参考方法之间的相关性极佳(rs = 0.95),比例偏差为 4.5%。TEobs介于7.0%和9.8%之间。尽管两者的结果非常一致,但仍有15%的样本出现了甲状腺状态不一致的结果:POCA的分析性能优异,与参考方法的相关性也很好。除了血红蛋白的干扰效应外,TEobs 在所有分析中都表现良好。不建议对严重溶血的样本进行分析。不过,由于 POCA 的动态范围相对较小,无法对 TT4 >8 μg/dL 的样本进行定量分析,因此需要重新建立参考区间。
{"title":"Evaluation of the analytical performance of a point-of-care analyzer for the measurement of feline serum thyroxine concentration in comparison with a chemiluminescence enzyme immunoassay.","authors":"Agnes C Gläsel, Kristina Weiler, Alexander Pankraz, Natali Bauer","doi":"10.1111/vcp.13416","DOIUrl":"https://doi.org/10.1111/vcp.13416","url":null,"abstract":"<p><strong>Background: </strong>Total thyroxine (TT4) measurement is used to assess thyroid status in cats.</p><p><strong>Objectives: </strong>The aim of the prospective study was to evaluate the analytical performance of the point-of-care analyzer (POCA) Immuno AU10V using the v-T4 test kit for feline TT4 measurement. Additionally, method comparison with a benchtop analyzer (IMMULITE 2000) was done.</p><p><strong>Methods: </strong>Validation included linearity, inter- and intra-assay precision, precision near the lower limit of quantification (LloQ), and interference testing for hemoglobin, lipid, and bilirubin. Correlation and bias were assessed.</p><p><strong>Results: </strong>Linearity was given within the dynamic range. Coefficients of variation (CV) were ≤4% near the LloQ as well as for intra-and inter-assay precision. No interference was observed for lipid and bilirubin, while hemoglobin caused a negative bias of 28%. Method comparison included 74 samples within three TT4 concentration ranges (0.5-3.7, >3.7-5.13, >5.13-8 μg/dL). Correlation between POCA and reference method was excellent (r<sub>s</sub> = 0.95) with a slight proportional bias of 4.5%. TE<sub>obs</sub> was between 7.0% and 9.8%. Despite substantial agreement, discordant results on thyroid status occurred in 15% of samples.</p><p><strong>Conclusions: </strong>The analytical performance of the POCA was excellent, as was its correlation with the reference method. Except for the interferent effect of hemoglobin, the TE<sub>obs</sub> was <TE<sub>a</sub> for all analyses. Analysis of severely hemolytic samples is not advised. However, the relatively small dynamic range of the POCA precludes quantitative analysis of samples with TT4 >8 μg/dL, and de novo reference intervals need to be established.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
What is your diagnosis? Fine-needle aspirate from a subcutaneous preputial mass in a ferret.
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-02-10 DOI: 10.1111/vcp.13414
Caitlyn F Connor, Jesse Riker, Laura K Burns, Jörg Mayer, Jesse M Hostetter, Katie Metcalf
{"title":"What is your diagnosis? Fine-needle aspirate from a subcutaneous preputial mass in a ferret.","authors":"Caitlyn F Connor, Jesse Riker, Laura K Burns, Jörg Mayer, Jesse M Hostetter, Katie Metcalf","doi":"10.1111/vcp.13414","DOIUrl":"https://doi.org/10.1111/vcp.13414","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of hematologic reference intervals for free-living King vultures (Sarcoramphus papa).
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-02-02 DOI: 10.1111/vcp.13415
Enzo Basso, Diego Rolim Chulla, Eduardo Tubelli, Sarah Wicks, Eleanor Flatt, Christopher Beirne, Andrew Whitworth

Background: As obligate scavengers, New World vultures (Cathartiformes: Cathartidae) play a key role in carcass removal and disease control. Associated with this ecosystem service, vultures are exposed to the consumption of harmful substances such as poisons, heavy metals, antibiotics, or non-steroidal anti-inflammatory drugs. Monitoring the health status of vulture populations is a priority and an important conservation strategy, and hematologic analysis is a practical and effective method that can be useful for this purpose.

Objectives: Report hematologic reference intervals in free-living King vultures (Sarcoramphus papa), one of the least studied New World vulture species.

Methods: Red blood cell concentration, packed cell volume, hemoglobin (Hb) concentration, MCV, MCH, MCHC, WBC concentration, differential count and absolute WBC concentration, heterophil to lymphocyte ratio, and total protein concentration were determined. We used a non-parametric method to calculate the reference intervals. In addition, we compared our results with the hematologic measurands reported for the Black vulture (Coragyps atratus), the Andean condor (Vultur gryphus) and the California condor (Gymnogyps californianus).

Results: With the exception of Hb, monocyte, basophil, and eosinophil concentrations, the mean hematologic measurands observed in the King vulture were similar to those recorded for the other New World vulture species, and the heterophil to lymphocyte ratio was lower in the King vulture compared to the Black vulture.

Conclusions: In this study, we have reported for the first time the hematologic reference intervals in a free-living population of King vultures in Costa Rica. Future research should consider comparison among free-living New World vulture species.

{"title":"Determination of hematologic reference intervals for free-living King vultures (Sarcoramphus papa).","authors":"Enzo Basso, Diego Rolim Chulla, Eduardo Tubelli, Sarah Wicks, Eleanor Flatt, Christopher Beirne, Andrew Whitworth","doi":"10.1111/vcp.13415","DOIUrl":"https://doi.org/10.1111/vcp.13415","url":null,"abstract":"<p><strong>Background: </strong>As obligate scavengers, New World vultures (Cathartiformes: Cathartidae) play a key role in carcass removal and disease control. Associated with this ecosystem service, vultures are exposed to the consumption of harmful substances such as poisons, heavy metals, antibiotics, or non-steroidal anti-inflammatory drugs. Monitoring the health status of vulture populations is a priority and an important conservation strategy, and hematologic analysis is a practical and effective method that can be useful for this purpose.</p><p><strong>Objectives: </strong>Report hematologic reference intervals in free-living King vultures (Sarcoramphus papa), one of the least studied New World vulture species.</p><p><strong>Methods: </strong>Red blood cell concentration, packed cell volume, hemoglobin (Hb) concentration, MCV, MCH, MCHC, WBC concentration, differential count and absolute WBC concentration, heterophil to lymphocyte ratio, and total protein concentration were determined. We used a non-parametric method to calculate the reference intervals. In addition, we compared our results with the hematologic measurands reported for the Black vulture (Coragyps atratus), the Andean condor (Vultur gryphus) and the California condor (Gymnogyps californianus).</p><p><strong>Results: </strong>With the exception of Hb, monocyte, basophil, and eosinophil concentrations, the mean hematologic measurands observed in the King vulture were similar to those recorded for the other New World vulture species, and the heterophil to lymphocyte ratio was lower in the King vulture compared to the Black vulture.</p><p><strong>Conclusions: </strong>In this study, we have reported for the first time the hematologic reference intervals in a free-living population of King vultures in Costa Rica. Future research should consider comparison among free-living New World vulture species.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and cats.
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-01-26 DOI: 10.1111/vcp.13412
Candice P Chu, Mary B Nabity

MicroRNAs (miRNAs or miRs) are small, non-coding RNAs that play a crucial role in gene regulation, making them potential biomarkers for various diseases. In the field of veterinary medicine, there is a growing interest in exploring the diagnostic and therapeutic potential of miRNAs in kidney diseases affecting dogs and cats. This review focuses on the use of urinary miRNAs as biomarkers for chronic kidney disease (CKD) in these companion animals. We introduce miRNAs, their biogenesis, and their presence in biofluids, particularly within exosomes, and discuss studies investigating miRNAs in kidney tissue and urine. We acknowledge the challenges associated with miRNA studies, including preanalytical factors such as biological variation, sample collection/processing, storage conditions, and experimental design. We highlight the importance of technical considerations, such as sample pooling, sequencing depth, multiplexing, and the various steps of the miRNA experimental workflow. Furthermore, we discuss RNA isolation methods, small RNA sequencing data analysis, and the use of quantitative reverse transcription PCR (qRT-PCR) and droplet digital PCR for verification. We emphasize the importance of internal controls, spike-ins, and normalization methods to minimize technical variation and ensure reliable results in qRT-PCR analysis. This review concludes that while urinary miRNAs hold promise as non-invasive biomarkers for CKD in dogs and cats, addressing the challenges and standardization of protocols is vital for the successful translation of this research into clinical practice.

{"title":"Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and cats.","authors":"Candice P Chu, Mary B Nabity","doi":"10.1111/vcp.13412","DOIUrl":"https://doi.org/10.1111/vcp.13412","url":null,"abstract":"<p><p>MicroRNAs (miRNAs or miRs) are small, non-coding RNAs that play a crucial role in gene regulation, making them potential biomarkers for various diseases. In the field of veterinary medicine, there is a growing interest in exploring the diagnostic and therapeutic potential of miRNAs in kidney diseases affecting dogs and cats. This review focuses on the use of urinary miRNAs as biomarkers for chronic kidney disease (CKD) in these companion animals. We introduce miRNAs, their biogenesis, and their presence in biofluids, particularly within exosomes, and discuss studies investigating miRNAs in kidney tissue and urine. We acknowledge the challenges associated with miRNA studies, including preanalytical factors such as biological variation, sample collection/processing, storage conditions, and experimental design. We highlight the importance of technical considerations, such as sample pooling, sequencing depth, multiplexing, and the various steps of the miRNA experimental workflow. Furthermore, we discuss RNA isolation methods, small RNA sequencing data analysis, and the use of quantitative reverse transcription PCR (qRT-PCR) and droplet digital PCR for verification. We emphasize the importance of internal controls, spike-ins, and normalization methods to minimize technical variation and ensure reliable results in qRT-PCR analysis. This review concludes that while urinary miRNAs hold promise as non-invasive biomarkers for CKD in dogs and cats, addressing the challenges and standardization of protocols is vital for the successful translation of this research into clinical practice.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing artificial intelligence for enhanced veterinary diagnostics: A look to quality assurance, Part II External validation.
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-01-22 DOI: 10.1111/vcp.13407
Christina Pacholec, Bente Flatland, Hehuang Xie, Kurt Zimmerman

Artificial intelligence (AI) is emerging as a valuable diagnostic tool in veterinary medicine, offering affordable and accessible tests that can match or even exceed the performance of medical professionals in similar tasks. Despite the promising outcomes of using AI systems (AIS) as highly accurate diagnostic tools, the field of quality assurance in AIS is still in its early stages. Our Part I manuscript focused on the development and technical validation of an AIS. In Part II, we explore the next step in development: external validation (i.e., in silico testing). This phase is a critical quality assurance component for any AIS intended for medical use, ensuring that high-quality diagnostics remain the standard in veterinary medicine. The quality assurance process for evaluating an AIS involves rigorous: (1) investigation of sources of bias, (2) application of calibration methods and prediction of uncertainty, (3) implementation of safety monitoring systems, and (4) assessment of repeatability and robustness. Testing with unseen data is an essential part of in silico testing, as it ensures the accuracy and precision of the AIS output.

{"title":"Harnessing artificial intelligence for enhanced veterinary diagnostics: A look to quality assurance, Part II External validation.","authors":"Christina Pacholec, Bente Flatland, Hehuang Xie, Kurt Zimmerman","doi":"10.1111/vcp.13407","DOIUrl":"https://doi.org/10.1111/vcp.13407","url":null,"abstract":"<p><p>Artificial intelligence (AI) is emerging as a valuable diagnostic tool in veterinary medicine, offering affordable and accessible tests that can match or even exceed the performance of medical professionals in similar tasks. Despite the promising outcomes of using AI systems (AIS) as highly accurate diagnostic tools, the field of quality assurance in AIS is still in its early stages. Our Part I manuscript focused on the development and technical validation of an AIS. In Part II, we explore the next step in development: external validation (i.e., in silico testing). This phase is a critical quality assurance component for any AIS intended for medical use, ensuring that high-quality diagnostics remain the standard in veterinary medicine. The quality assurance process for evaluating an AIS involves rigorous: (1) investigation of sources of bias, (2) application of calibration methods and prediction of uncertainty, (3) implementation of safety monitoring systems, and (4) assessment of repeatability and robustness. Testing with unseen data is an essential part of in silico testing, as it ensures the accuracy and precision of the AIS output.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correspondence regarding the article, Sysmex XN-V hematology analyzer for feline specimens 关于文章的通信,Sysmex XN-V猫标本血液学分析仪。
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-01-15 DOI: 10.1111/vcp.13402
Hinpetch Daungsupawong, Viroj Wiwanitkit
{"title":"Correspondence regarding the article, Sysmex XN-V hematology analyzer for feline specimens","authors":"Hinpetch Daungsupawong,&nbsp;Viroj Wiwanitkit","doi":"10.1111/vcp.13402","DOIUrl":"10.1111/vcp.13402","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"383"},"PeriodicalIF":1.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response to Letter to the editor 回复给编辑的信。
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-01-15 DOI: 10.1111/vcp.13406
Fanny Granat, Jean-Pierre Braun, Nathalie Bourgès-Abella, Catherine Trumel
{"title":"Response to Letter to the editor","authors":"Fanny Granat,&nbsp;Jean-Pierre Braun,&nbsp;Nathalie Bourgès-Abella,&nbsp;Catherine Trumel","doi":"10.1111/vcp.13406","DOIUrl":"10.1111/vcp.13406","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"384"},"PeriodicalIF":1.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical atlas of small animal cytology and hematology ( 2nd ed.) by AG Burton (Ed.), Hoboken, NJ, USA: Wiley-Blackwell. 2024. ISBN: 978-1119985624. $114
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-01-15 DOI: 10.1111/vcp.13385
Francesco Cian
{"title":"Clinical atlas of small animal cytology and hematology ( 2nd ed.) by AG Burton (Ed.), Hoboken, NJ, USA: Wiley-Blackwell. 2024. ISBN: 978-1119985624. $114","authors":"Francesco Cian","doi":"10.1111/vcp.13385","DOIUrl":"https://doi.org/10.1111/vcp.13385","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"458-459"},"PeriodicalIF":1.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143252660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytauxzoon felis: An overlooked diagnostic test 猫胞虫:一种被忽视的诊断试验。
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2025-01-03 DOI: 10.1111/vcp.13411
Laura A. Cagle, Sarah Bosch, Rachel Whitman, Shir Gilor, Christopher Lanier
{"title":"Cytauxzoon felis: An overlooked diagnostic test","authors":"Laura A. Cagle,&nbsp;Sarah Bosch,&nbsp;Rachel Whitman,&nbsp;Shir Gilor,&nbsp;Christopher Lanier","doi":"10.1111/vcp.13411","DOIUrl":"10.1111/vcp.13411","url":null,"abstract":"","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":"53 4","pages":"381-382"},"PeriodicalIF":1.2,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrophoretic fractionations of lipoproteins in dogs: Intra- and inter-assay imprecision and effects of different storage conditions. 犬脂蛋白的电泳分离:测定内和测定间的不精确性和不同储存条件的影响。
IF 1.2 4区 农林科学 Q3 VETERINARY SCIENCES Pub Date : 2024-12-29 DOI: 10.1111/vcp.13405
Saverio Paltrinieri, Gabriele Rossi, Alessia Giordano

Background: Lipoprotein fractions are reported to be unstable in stored human samples, and there is a paucity of information on the analytical precision of electrophoretic separation of lipoproteins in canine serum samples.

Objective: The aim of this study was to assess the effects of intra- and inter-assay imprecision and of storage conditions on the electrophoretic separation of canine lipoproteins.

Methods: Imprecision was assessed by calculating the coefficient of variation (CV) of five replicates of six serum samples run in two sequential runs of agarose gel lipoprotein electrophoresis. The effect of storage was assessed with a Friedmann test by comparing the results of samples analyzed after sampling (T0) and after 24 and 48 h at room temperature or stored at 4°C and after 7, 14, 21 days, 1, 2, and 3 months at -20°C or at -80°C. Moreover, electrophoretograms obtained after storage were visually analyzed by two observers in a blind manner to assess whether storage alters the electrophoretic profile.

Results: The imprecision of high-density lipoproteins (HDL), low-density lipoproteins (LDL), very low-density lipoprotein (VLDL), and chylomicrons were respectively 0.8%-11.5%, 2.4%-22.7%, 2.3%-11.5%, and 12.5%-105.2%. Compared with T0, HDL significantly decreased, and LDL significantly increased over time in all the storage conditions, whereas VLDL significantly increased only in frozen samples, and chylomicrons did not significantly differ. In frozen samples, deviations from baseline values were lower than the imprecision of the method, and visual misclassifications of electrophoretograms were rare.

Conclusions: Despite minimal variation in the percentage of some fractions, freezing does not influence the interpretation of canine lipidograms.

背景:据报道,在储存的人类样品中,脂蛋白部分是不稳定的,并且在犬血清样品中脂蛋白电泳分离的分析精度方面缺乏信息。目的:研究犬类脂蛋白电泳分离过程中,测定内、测定间不精确度和储存条件对电泳分离的影响。方法:通过计算琼脂糖凝胶脂蛋白电泳5个重复的变异系数(CV)来评估不精确度。通过比较取样后(T0)、室温保存24和48小时、4℃保存和-20°C或-80°C保存7、14、21天、1、2和3个月后的结果,采用Friedmann试验评估贮藏效果。此外,储存后获得的电泳图由两名观察者以盲目的方式进行视觉分析,以评估储存是否改变了电泳谱。结果:高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、极低密度脂蛋白(VLDL)和乳糜微粒的不精确性分别为0.8% ~ 11.5%、2.4% ~ 22.7%、2.3% ~ 11.5%和12.5% ~ 105.2%。与T0相比,在所有贮藏条件下,随着时间的推移,HDL显著降低,LDL显著升高,而VLDL仅在冷冻条件下显著升高,乳糜微粒无显著差异。在冷冻样品中,与基线值的偏差低于方法的不精确性,电泳图的视觉错误分类是罕见的。结论:尽管某些组分的百分比变化很小,但冷冻并不影响犬脂质图的解释。
{"title":"Electrophoretic fractionations of lipoproteins in dogs: Intra- and inter-assay imprecision and effects of different storage conditions.","authors":"Saverio Paltrinieri, Gabriele Rossi, Alessia Giordano","doi":"10.1111/vcp.13405","DOIUrl":"https://doi.org/10.1111/vcp.13405","url":null,"abstract":"<p><strong>Background: </strong>Lipoprotein fractions are reported to be unstable in stored human samples, and there is a paucity of information on the analytical precision of electrophoretic separation of lipoproteins in canine serum samples.</p><p><strong>Objective: </strong>The aim of this study was to assess the effects of intra- and inter-assay imprecision and of storage conditions on the electrophoretic separation of canine lipoproteins.</p><p><strong>Methods: </strong>Imprecision was assessed by calculating the coefficient of variation (CV) of five replicates of six serum samples run in two sequential runs of agarose gel lipoprotein electrophoresis. The effect of storage was assessed with a Friedmann test by comparing the results of samples analyzed after sampling (T0) and after 24 and 48 h at room temperature or stored at 4°C and after 7, 14, 21 days, 1, 2, and 3 months at -20°C or at -80°C. Moreover, electrophoretograms obtained after storage were visually analyzed by two observers in a blind manner to assess whether storage alters the electrophoretic profile.</p><p><strong>Results: </strong>The imprecision of high-density lipoproteins (HDL), low-density lipoproteins (LDL), very low-density lipoprotein (VLDL), and chylomicrons were respectively 0.8%-11.5%, 2.4%-22.7%, 2.3%-11.5%, and 12.5%-105.2%. Compared with T0, HDL significantly decreased, and LDL significantly increased over time in all the storage conditions, whereas VLDL significantly increased only in frozen samples, and chylomicrons did not significantly differ. In frozen samples, deviations from baseline values were lower than the imprecision of the method, and visual misclassifications of electrophoretograms were rare.</p><p><strong>Conclusions: </strong>Despite minimal variation in the percentage of some fractions, freezing does not influence the interpretation of canine lipidograms.</p>","PeriodicalId":23593,"journal":{"name":"Veterinary clinical pathology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Veterinary clinical pathology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1