Cycloisolongifolene-8,9-Dehydro-9-Formyl Inhibits Lipoxygenase and Might Play a Role in the Wound-Healing Property of Clerodendrum infortunatum and Tagetes erecta
Bhavana Sampath Kumar, Sandra Ravi M, Junaiha Kapoor, Atheene Joy, Elcey C. Daniel, Shinomol George K, Deepu Joy Parayil, Dileep Francis
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引用次数: 0
Abstract
Introduction
Clerodendrum infortunatum, and Tagetes erecta, are frequently used in traditional medicinal systems like Ayurveda and Unani for their medicinal properties. This study explores their wound-healing potential.
Methods
Ethanol extracts were obtained from C infortunatum leaves and T erecta flowers and subjected to qualitative phytochemical analyses. . The antioxidant properties were assessed through 2,2-diphenyl-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays, both individually and combined. Cytotoxicity was tested using the MTT assay. An in vitro lipoxygenase (LOX) inhibition assay was used to evaluate anti-inflammatory activity. Extracts were further fractionated using silica gel column chromatography and assayed for LOX-inibition. Metabolite profiling of the active fractions were performed using GC-MS. Molecular docking was employed to study the interactions of the identified metabolites with LOX.
Results
The plant extracts exhibited substantial scavenging activity (76–80% in the DPPH assay). FRAP values were 415.31 µg ascorbic acid/g DW for C infortunatum and 1185.86 µg ascorbic acid/g DW for T erecta. The extracts and fractions showed moderate cytotoxicity, with the highest non-toxic concentration being 25 µg/mL. LOX inhibition was 54% for C infortunatum, and 56% for T erecta, comparable to vanillin (55%). Purified fractions achieved 70% (C infortunatum) and 76% (T erecta) inhibition. Proliferation assays revealed significant wound-healing activities of 76% (C infortunatum) and 90% (T erecta) at 25 µg/mL, compared to the control (50%). GC-MS identified the key metabolite, cycloisolongifolene-8,9-dehydro-9-formyl, in both fractions. Molecular docking provided mechanistic insights into LOX inhibition.
Conclusions
C infortunatum (leaf) and T erecta (flower) extracts possess remarkable wound-healing potential. The identified metabolite, cycloisolongifolene-8,9-dehydro-9-formyl, likely contributes to wound healing. The findings highlight the therapeutic potential of the plant extracts in wound management and warrant further investigation into the active principle/s involved in the mechanism.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.