José Alcides Almeida de Arruda, Sicília Rezende Oliveira, Fernanda Vieira Heimlich, Bárbara Maria de Amorim-Santos, Ayda Henriques Schneider, Ana Carolina Velasco Pondé de Sena, Karla Emília de Sá Rodrigues, Soraia Macari, Daniele Glória Souza, Denise Vieira Travassos, Lucas Guimarães Abreu, Tarcília Aparecida Silva, Ricardo Alves Mesquita
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引用次数: 0
Abstract
Objective: This study investigated the concentrations of neutrophil extracellular traps (NET) and salivary cytokines (IL-1β, IL-6, IL-8/CXCL8, TNF, and TGF-β1) in patients undergoing chemotherapy and their associations with oral mucositis (OM) and Candida infection.
Materials and methods: This prospective longitudinal study performed at a Brazilian service included 60 adults diagnosed with hematolymphoid diseases. Saliva samples were collected on days D0, D3, D10, and D15. Cytokines were analyzed by ELISA and NET formation by identification of the myeloperoxidase-DNA complex. Oral Candida spp. was cultured.
Results: OM occurred in 43.3% of patients and oral candidiasis in 20%. However, 66% of individuals had positive cultures for C. albicans. Higher concentrations of IL-6, IL-8/CXCL8, and TNF and lower concentrations of TGF-β1 were observed in patients with OM. C. albicans infection contributed to the increase in IL-8/CXCL8, TGF-β1, and TNF. Individuals with OM or with oral candidiasis had significant reductions in NET formation. In contrast, individuals with C. albicans and with concomitant C. albicans and OM exhibited higher NET formation.
Conclusion: The kinetics of cytokine levels and NET formation in chemotherapy-induced OM appears to be altered by Candida infection, even in the absence of clinical signs of oral candidiasis.
研究目的本研究调查了化疗患者体内中性粒细胞胞外捕获物（NET）和唾液细胞因子（IL-1β、IL-6、IL-8/CXCL8、TNF和TGF-β1）的浓度及其与口腔粘膜炎（OM）和念珠菌感染的关系：这项前瞻性纵向研究是在巴西的一家医疗机构进行的，共纳入了 60 名被诊断患有血淋巴疾病的成年人。研究人员在 D0、D3、D10 和 D15 天采集了唾液样本。通过 ELISA 分析细胞因子，通过髓过氧化物酶-DNA 复合物鉴定 NET 的形成。对口腔念珠菌进行培养：结果：43.3%的患者患有口腔黏膜炎，20%的患者患有口腔念珠菌病。然而，66%的患者白念珠菌培养呈阳性。在 OM 患者中观察到较高浓度的 IL-6、IL-8/CXCL8 和 TNF，以及较低浓度的 TGF-β1。白念珠菌感染导致了 IL-8/CXCL8、TGF-β1 和 TNF 的增加。OM患者或口腔念珠菌感染者的NET形成显著减少。相比之下，白念珠菌感染者以及同时感染白念珠菌和 OM 的患者的 NET 形成率更高：结论：即使没有口腔念珠菌病的临床症状，化疗诱导的 OM 中细胞因子水平和 NET 形成的动力学似乎也会受到念珠菌感染的影响。
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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