Identification and characterization of sub-group 4 R2R3-MYB members and their functions in phenylpropanoid and flavonoid regulation in tobacco

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2023-12-28 DOI:10.1016/j.cpb.2023.100317
Yinchao Zhang , Xiuchun Li , Xiaoling Qu , Min Ren , Ying Tong , Xiuming Wu , Yangyang Sun , Jun Yang , Fengyan Wu , Aiguo Yang , Shuai Chen
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Abstract

Sub-group 4 R2R3-type MYB transcription factors (TFs) are involved in repression of the phenylpropanoid pathway. In this study, we identified and characterized three MYB transcriptional repressors in common tobacco, named NtMYB1, NtMYB2, and NtMYB4, which belong to sub-group 4 members. Tissue expression properties and specificity of stress-induced expression indicated that the NtMYB1, NtMYB2, and NtMYB4 may perform different functions in tobacco. Overexpression of NtMYB1, NtMYB2, and NtMYB4 reduced phenylpropanoids and flaonoid compounds, such as caffeic acid, chlorogenic acid, neochlorogenic acid, and rutin, respectively. Meanwhile, the expression of the most structural genes involved in phenylpropanoids and flavonoids were reduced in the overexpression transgenic lines. In addition, results based on co-expression and expression analyses revealed possible interactions between NtMYB1, NtMYB2, and NtMYB4 and multiple bHLH TFs. Our results showed that the three sub-group 4 R2R3 members exhibited different expression pattern in various tissues and under abiotic stress conditions. Meanwhile, we hypothesized that the three of them involved in the inhibition of phenylpropanoid and flavonoid biosynthesis might be interaction with bHLH TFs.

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第 4 亚群 R2R3-MYB 成员的鉴定和表征及其在烟草中调节苯丙类和黄酮类化合物的功能
第 4 亚群 R2R3 型 MYB 转录因子(TFs)参与抑制苯丙酮途径。本研究发现并鉴定了普通烟草中的三种 MYB 转录抑制因子,分别命名为 NtMYB1、NtMYB2 和 NtMYB4,它们属于亚群 4 成员。组织表达特性和胁迫诱导表达的特异性表明,NtMYB1、NtMYB2 和 NtMYB4 可能在烟草中发挥不同的功能。过量表达 NtMYB1、NtMYB2 和 NtMYB4 会分别减少咖啡酸、绿原酸、新绿原酸和芦丁等苯丙类和类黄酮化合物。同时,在过表达转基因品系中,参与苯丙酮类化合物和黄酮类化合物的大部分结构基因的表达量都有所降低。此外,基于共表达和表达分析的结果显示,NtMYB1、NtMYB2和NtMYB4与多个bHLH TFs之间可能存在相互作用。我们的研究结果表明,R2R3第4亚群的三个成员在不同组织和非生物胁迫条件下表现出不同的表达模式。同时,我们推测它们三者参与抑制苯丙类和黄酮类化合物的生物合成可能是与 bHLH TFs 相互作用的结果。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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