Identification of Chlamydia abortus and Chlamydia pecorum in water buffaloes (Bubalus bubalis) and cows cohabitating the same herd

IF 0.2 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Buffalo Bulletin Pub Date : 2023-12-31 DOI:10.56825/bufbu.2023.4243989
Magdalena Limón–González, R. Hernández-Castro, Gabriela Palomares Reséndiz, Enrique Herrera López, Efrén Díaz Aparicio
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Abstract

The aim of this study was to determine the presence of Chlamydia spp. in a dual-purpose zebu and water buffalo-mixed herd cohabitating in a ranch located in southern Mexico. The zootechnical purpose of this herd is milk and cheese production. A total of 52 vaginal exudate samples were obtained from clinically healthy water buffaloes, less than one month after parturition, and two vaginal exudate samples were taken from cows that had recently aborted. L929 cells were used for bacterial isolation. Two of these cultures were infected, confirmed by direct immunofluorescence. Total DNA was extracted for analysis with two types of real-time PCR, a Chlamydiaceae-specific real-time PCR and another species-specific real-time PCR for C. abortus, C. psittaci and C. pecorum. Two Chlamydia isolates were obtained, one from a water buffalo positive to C. abortus and C. pecorum, the other from a cow positive to C. abortus. This is the first report of the presence of C. abortus and C. pecorum in water buffaloes in Mexico. C. abortus was also detected in one cow living in the same herd as the water buffaloes.
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鉴定同群水牛和奶牛体内的流产衣原体和啄木鸟衣原体
本研究的目的是确定墨西哥南部一个牧场中的斑马和水牛混群中是否存在衣原体。该牛群的饲养目的是生产牛奶和奶酪。从产后不到一个月的临床健康水牛身上共采集了 52 份阴道渗出物样本,并从最近流产的奶牛身上采集了两份阴道渗出物样本。使用 L929 细胞进行细菌分离。经直接免疫荧光法确认,其中两个培养物受到感染。提取总 DNA 进行两种实时 PCR 分析:一种是衣原体特异性实时 PCR,另一种是流产衣原体、鹦鹉热衣原体和啄木鸟衣原体的物种特异性实时 PCR。结果发现了两种衣原体分离物,一种来自对流产衣原体和啄木鸟衣原体呈阳性反应的水牛,另一种来自对流产衣原体呈阳性反应的奶牛。这是墨西哥首次报告水牛体内存在流产衣原体和啄木鸟衣原体。在与水牛生活在同一牛群中的一头奶牛身上也检测到了堕胎杆菌。
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来源期刊
Buffalo Bulletin
Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
0.50
自引率
0.00%
发文量
53
审稿时长
>12 weeks
期刊介绍: Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.
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