Changes in the germination and content of protein and gluten in the caryopses of hard and soft wheat under pulse pressure and prolonged storage conditions

Ya. I. Khramova, E. Nefed’eva, S. Belopukhov, I. Dmitrevskaya, V. Khramova
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Abstract

The study aims to identify the aging characteristics of hard and soft wheat caryopses without treatment and following treatment with pulse pressure created by a shock wave. The grain was treated with pulse pressures of 11 and 29 MPa. After one month and after three years, the caryopses were sorted into vitreous and floury types. The germination of control soft wheat caryopses decreased after storage, while after pulse pressure treatment and storage, it exceeded that of the control. The germination of control hard wheat caryopses with floury endosperm decreased after storage to a greater extent than that of caryopses with vitreous endosperm. Pulse pressure treatment reduced the germination of caryopses with vitreous endosperm. No change was observed in the protein content of control hard wheat caryopses following prolonged storage. In this case, pulse pressure contributed to the reduction in protein content during storage. The protein content of soft wheat caryopses with floury endosperm decreased after storage. In these caryopses, pulse pressure treatment contributed to better protein retention. Thus, the caryopses of hard wheat and soft wheat with vitreous endosperm were less susceptible to aging as compared to the caryopses of soft wheat and caryopses with floury endosperm, respectively. Pulse pressure damaged the vitreous endosperm, accelerating seed aging. It also contributed to the long-term storage of soft wheat caryopses without a significant loss of viability. Thus, it is possible to treat soft wheat grain to extend its shelf life.
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脉冲压力和长期储存条件下硬质小麦和软质小麦颖果的发芽及蛋白质和面筋含量的变化
这项研究旨在确定未经处理和经冲击波产生的脉冲压力处理后的硬质和软质小麦颖果的陈化特性。谷物分别以 11 和 29 兆帕的脉冲压力进行处理。一个月后和三年后,将麦粒分为玻璃质和絮状两种类型。对照组软质小麦核果在储存后发芽率下降,而经过脉冲加压处理和储存后,发芽率超过了对照组。胚乳为絮状的对照硬质小麦果穗在贮藏后的发芽率比胚乳为玻璃质的果穗的发芽率下降得更多。脉冲压力处理降低了具有玻璃质胚乳的硬粒小麦的发芽率。长期储存后,对照硬质小麦核果的蛋白质含量没有变化。在这种情况下,脉冲压力导致了贮藏过程中蛋白质含量的降低。胚乳呈绒毛状的软质小麦在贮藏后蛋白质含量下降。在这些小麦胚乳中,脉冲压力处理有助于更好地保留蛋白质。因此,硬质小麦和软质小麦胚乳玻璃质的子房分别比软质小麦和胚乳呈绒毛状的子房更不易老化。脉冲压力会破坏玻璃质胚乳,加速种子老化。脉冲压力还有助于软质小麦果仁的长期储藏,而不会明显降低其存活率。因此,对软麦粒进行处理以延长其货架期是可行的。
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