Fabrication and evaluation of some characteristics of the skin's dermis gel direction to application in regenerative medicine

Abstract

Objective:The tight connection between the cell-extracellular matrix (ECM - Extracellular matrix) is the foundation of tissue development and repair. The extracellular framework simulates the biological properties of organism-based ECM. The ECM of the skin’s dermis is a natural material, containing ingredients ideal for creating products that help tissue heal. This study focuses on the production of gel products from human skin ECM. The skin ECM is gelled by dissolving in a pepsin enzyme solution.Method: 3D gel formation process using neutral enzyme solution and incubation under physiological conditions. Gel samples were evaluated for speed and formability. Additionally, the dermal gel preparation was evaluated for its effects on the survival and proliferation of human fibroblasts in vitro by toxicity testing and proliferation testing, for 10 days. Results: Direct and extract toxicity assessments showed that the ECM gel regimen was safe for fibroblast survival. At the same time, fibroblasts had proliferation properties after 10 days in culture conditions with gel extract.Conclusion: These results showed that the ECM gel product regimen has the ability to positively impact the biological properties of cells and has the potential for application in the field of regenerative medicine.