Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).20-24
Thanh Loan Pham
The active ingredient catalpol in the Rehmannia glutinosavariety 19 roots is an important chemical component. Catalpol has hypoglycemia, diuretic, and laxative effects; it is used to support the treatment of diabetes, ischemic cerebral, and dementia, and it is regulated by Vietnamese Pharmacopoeia V (2017) as a marker to check the quality of medicinal herbs. This study aimed to develop a procedure for quantifying catalpol in the root of Rehmannia glutinosa varieties 19 by high-performance liquid chromatography (HPLC) to evaluate the quality of medicinal herbs. The research results have selected suitable chromatographic conditions using the Gemini C18 column (250x4.6 mm, 5 μm), detector at 210 nm, mobile phase acetonitrile - phosphoric acid 0.1%, and flow rate at 0.6 ml/min. The peak area and catalpol concentration have a strong linear correlation with r=0.9999, Y = 21887X + 26213. The procedure has good accuracy and repeatability with a relative standard deviation (RSD) <2%. Quantitative results of catalpol in the root of the R. glutinosa varieties 19 reached 1.25%. The HPLC method is suitable for quantifying catalpol in the root of R. glutinosa varieties 19.
{"title":"Quantification of catalpol in root of Rehmannia glutinosa varieties 19 collected in Phu Tho province by high-performance liquid chromatography","authors":"Thanh Loan Pham","doi":"10.31276/vjst.66(3).20-24","DOIUrl":"https://doi.org/10.31276/vjst.66(3).20-24","url":null,"abstract":"The active ingredient catalpol in the Rehmannia glutinosavariety 19 roots is an important chemical component. Catalpol has hypoglycemia, diuretic, and laxative effects; it is used to support the treatment of diabetes, ischemic cerebral, and dementia, and it is regulated by Vietnamese Pharmacopoeia V (2017) as a marker to check the quality of medicinal herbs. This study aimed to develop a procedure for quantifying catalpol in the root of Rehmannia glutinosa varieties 19 by high-performance liquid chromatography (HPLC) to evaluate the quality of medicinal herbs. The research results have selected suitable chromatographic conditions using the Gemini C18 column (250x4.6 mm, 5 μm), detector at 210 nm, mobile phase acetonitrile - phosphoric acid 0.1%, and flow rate at 0.6 ml/min. The peak area and catalpol concentration have a strong linear correlation with r=0.9999, Y = 21887X + 26213. The procedure has good accuracy and repeatability with a relative standard deviation (RSD) <2%. Quantitative results of catalpol in the root of the R. glutinosa varieties 19 reached 1.25%. The HPLC method is suitable for quantifying catalpol in the root of R. glutinosa varieties 19.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140382208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).25-30
Minh Tu To, Thi Xuyen Do, Thi Tuyet Hoang, Van Truong Phan, Minh Tu Dang, Thanh Huyen Pham
Objectives: To describe the morphological characteristics, anatomy (stem, leaves), and quantify the corosolic acid in the leaves of Lagerstroemia calyculata Kurz collected in the Southeast region, Vietnam. Subjects and methods: Using the comparative morphological method to determine the scientific name of species. Studying the microscopic characteristics of Lagerstroemia calyculataKurz according to the research method of N. Ba (2009) [1] and quantifying the content of corosolic acid in the leaves according to the United States Pharmacopeia 2017 (USP 40), with adjustments to suit the conditions of the laboratory. Results: Lagerstroemia calyculata Kurz is tree, deciduous, height 30-35 m, smooth bark. Leaves lanceolate, lateral veins 9-11 pairs, hairy. Inflorescence paniculate, small white flowers. Capsules ovoid, 6-valved. Seeds are numerous, brown, and smooth. The stem has a bicollateral vascular bundle, conduction tissue arranged continuously, double phloem pillars and leaf veins exposed on both sides. The outer epidermis has multicellular stellate hairs. Scattered in soft tissue there are crystalline particles. Stomata are present only on the underside of leaves. The quantification of corosolic acid in three leaf samples of Lagerstroemia calyculata Kurz was from 0.05 to 0.12%. Conclusions: This study has detailed morphological, anatomical characteristics, and quantitative determination of corosolic acid in three leaf samples of Lagerstroemia calyculata Kurz collected in the Southeast region.
研究目的描述在越南东南部地区采集的 Lagerstroemia calyculata Kurz 的形态特征、解剖结构（茎、叶），并量化叶片中的科罗索酸。研究对象和方法用形态比较法确定物种学名。根据 N. Ba（2009 年）[1] 的研究方法研究 Lagerstroemia calyculataKurz 的显微特征，并根据《美国药典 2017》（USP 40）对叶片中的科罗索酸含量进行定量，并根据实验室条件进行调整。结果Lagerstroemia calyculata Kurz 为乔木，落叶，高 30-35 米，树皮光滑。叶披针形，侧脉 9-11 对，有毛。花序圆锥状，小白花。蒴果卵球形，6瓣裂。种子多数，褐色，光滑。茎有双侧维管束，传导组织连续排列，双韧皮部支柱，叶脉两侧外露。外表皮有多细胞星状毛。软组织中散布着结晶颗粒。气孔只出现在叶片背面。在 Lagerstroemia calyculata Kurz 的三个叶片样本中，科罗索酸的定量为 0.05% 到 0.12%。研究结论本研究对在东南地区采集的三份 Lagerstroemia calyculata Kurz 叶片样本中的科罗索酸进行了详细的形态、解剖特征和定量测定。
{"title":"Morphology, anatomy, and quantitative determination of corosolic acid in Lagerstroemia calyculata Kurz in the Southeast region, Vietnam","authors":"Minh Tu To, Thi Xuyen Do, Thi Tuyet Hoang, Van Truong Phan, Minh Tu Dang, Thanh Huyen Pham","doi":"10.31276/vjst.66(3).25-30","DOIUrl":"https://doi.org/10.31276/vjst.66(3).25-30","url":null,"abstract":"Objectives: To describe the morphological characteristics, anatomy (stem, leaves), and quantify the corosolic acid in the leaves of Lagerstroemia calyculata Kurz collected in the Southeast region, Vietnam. Subjects and methods: Using the comparative morphological method to determine the scientific name of species. Studying the microscopic characteristics of Lagerstroemia calyculataKurz according to the research method of N. Ba (2009) [1] and quantifying the content of corosolic acid in the leaves according to the United States Pharmacopeia 2017 (USP 40), with adjustments to suit the conditions of the laboratory. Results: Lagerstroemia calyculata Kurz is tree, deciduous, height 30-35 m, smooth bark. Leaves lanceolate, lateral veins 9-11 pairs, hairy. Inflorescence paniculate, small white flowers. Capsules ovoid, 6-valved. Seeds are numerous, brown, and smooth. The stem has a bicollateral vascular bundle, conduction tissue arranged continuously, double phloem pillars and leaf veins exposed on both sides. The outer epidermis has multicellular stellate hairs. Scattered in soft tissue there are crystalline particles. Stomata are present only on the underside of leaves. The quantification of corosolic acid in three leaf samples of Lagerstroemia calyculata Kurz was from 0.05 to 0.12%. Conclusions: This study has detailed morphological, anatomical characteristics, and quantitative determination of corosolic acid in three leaf samples of Lagerstroemia calyculata Kurz collected in the Southeast region.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140382536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).45-54
Van Duy Le, Thai The Nguyen, Hoang Phuong Tran, Anh Mai Nguyen
In this study, ionic liquid immobilised onto silica gel material SiO2-(CH2)3-N(Oct)3Br was successfully synthesised and characterised by Fourier-transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). This material was applied as the stationary phase of solid-phase extraction for the enrichment of trace amounts of carbamate pesticides in water. The cartridge was eluted with 5 ml of acetonitrile (ACN), blow-dried with nitrogen gas, and re-dissolved in ACN:H2O (40:60, v/v) and analysed by high-performance liquid chromatography - ultraviolet (HPLC-UV) at 210 and 245 nm. Within this process, elution of the cartridge with 5 ml of acetonitrile (ACN) was followed by nitrogen gas blow-drying and subsequent re-dissolution in ACN:H2O (40:60, v/v), culminating in analysis by HPLC-UV at 210 and 245 nm. Optimisation of conditions facilitated the analysis of carbamate pesticides, with the limit of quantitation (LOQ)ranging from 0.36-2.00 μg/l for tap water and 0.4-3.5 μg/l for river water. Furthermore, the method exhibited favourable repeatability, with a relative standard deviation (RSD) of less than 10%, thereby meeting the Association of Official Analytical Collaboration (AOAC) standards.
{"title":"Application of solid-phase extraction materials from ion liquids for the analysis of carbamate pesticide","authors":"Van Duy Le, Thai The Nguyen, Hoang Phuong Tran, Anh Mai Nguyen","doi":"10.31276/vjst.66(3).45-54","DOIUrl":"https://doi.org/10.31276/vjst.66(3).45-54","url":null,"abstract":"In this study, ionic liquid immobilised onto silica gel material SiO2-(CH2)3-N(Oct)3Br was successfully synthesised and characterised by Fourier-transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). This material was applied as the stationary phase of solid-phase extraction for the enrichment of trace amounts of carbamate pesticides in water. The cartridge was eluted with 5 ml of acetonitrile (ACN), blow-dried with nitrogen gas, and re-dissolved in ACN:H2O (40:60, v/v) and analysed by high-performance liquid chromatography - ultraviolet (HPLC-UV) at 210 and 245 nm. Within this process, elution of the cartridge with 5 ml of acetonitrile (ACN) was followed by nitrogen gas blow-drying and subsequent re-dissolution in ACN:H2O (40:60, v/v), culminating in analysis by HPLC-UV at 210 and 245 nm. Optimisation of conditions facilitated the analysis of carbamate pesticides, with the limit of quantitation (LOQ)ranging from 0.36-2.00 μg/l for tap water and 0.4-3.5 μg/l for river water. Furthermore, the method exhibited favourable repeatability, with a relative standard deviation (RSD) of less than 10%, thereby meeting the Association of Official Analytical Collaboration (AOAC) standards.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Faults are a type of geological structure that often leads to geological disasters in underground structures under normal conditions and especially when earthquakes occur, thereby requiring careful study.This article introduces some numerical simulation results on the seismic wave propagation process, on changes in stress and displacement states in the rock mass with a small fault of a dip angle of 45o, around a circular cross-section tunnel, using the discrete element method (Universal distinct element Code - UDEC). Three cases are simulated: a) The tunnel is located in the hanging wall rock, the fault is located 10m from the centre of the tunnel on the vertical axis; b) The fault runs through the centre of the tunnel and c) The tunnel is located in the footing wall rock, the fault is located 10m from the centre of the tunnel on the vertical axis. The results obtained show a very clear influence of the position and location of the fault on the geomechanical changes in the rock mass around the tunnel, contributing to the reasonable design of tunnels subjected to earthquakes.
{"title":"Prediction of geomechanical changes in faulted rock mass around underground structures subjected to earthquakes","authors":"Q. Nguyen, Ngoc Hue Nguyen, Quang Minh Nguyen, V. Nguyen, Tuan Minh Tran","doi":"10.31276/vjst.66(3).13-19","DOIUrl":"https://doi.org/10.31276/vjst.66(3).13-19","url":null,"abstract":"Faults are a type of geological structure that often leads to geological disasters in underground structures under normal conditions and especially when earthquakes occur, thereby requiring careful study.This article introduces some numerical simulation results on the seismic wave propagation process, on changes in stress and displacement states in the rock mass with a small fault of a dip angle of 45o, around a circular cross-section tunnel, using the discrete element method (Universal distinct element Code - UDEC). Three cases are simulated: a) The tunnel is located in the hanging wall rock, the fault is located 10m from the centre of the tunnel on the vertical axis; b) The fault runs through the centre of the tunnel and c) The tunnel is located in the footing wall rock, the fault is located 10m from the centre of the tunnel on the vertical axis. The results obtained show a very clear influence of the position and location of the fault on the geomechanical changes in the rock mass around the tunnel, contributing to the reasonable design of tunnels subjected to earthquakes.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).06-12
Doan Thanh Thuy Nguyen, De Nguyen, Huynh Trung Le Ly, Tuyet Phuong Nguyen
Raw TiO2 powder from a commercial source was used as a precursor in the hydrothermal process with deep eutectic solvent (DES) of choline chloride and urea with a molar ratio of 1:2 at different temperatures and interval times. The synthesised TiO2 was further utilised to produce diffracting TiO2 paste for application in dye-sensitised solar cells (DSCs) to improve the power conversation efficiency of devices. The TiO2 product analysed by X-ray diffraction (XRD) showed a similar phase and crystallinity to the raw material. However, scanning electron microscope (SEM) images showed that the hydrothermal TiO2 product had less particle aggregation than the raw one. The particle size was exceedingly 100 nm. The TiO2 pastes were prepared with a mixture of ethyl acetate and terpineol at volume ratios of 3:7 and 4:6. The paste was suitable to make thin films by the screen-printing method as a diffusion layer in cells. DSC devices using the TiO2 produced by the hydrothermal process with DES at 140oC for 18, 24, and 36 hours, respectively, enhanced the power conversion efficiency by 6.7-6.9%, which was higher than the ones using raw TiO2 by about 7%. Therefore, TiO2 powder modified by the hydrothermal process in DES solvent has great potential for application in DSC manufacture.
{"title":"Synthesis of TiO2 by hydrothermal method using deep eutectic solvent for application in dye-sensitised solar cell","authors":"Doan Thanh Thuy Nguyen, De Nguyen, Huynh Trung Le Ly, Tuyet Phuong Nguyen","doi":"10.31276/vjst.66(3).06-12","DOIUrl":"https://doi.org/10.31276/vjst.66(3).06-12","url":null,"abstract":"Raw TiO2 powder from a commercial source was used as a precursor in the hydrothermal process with deep eutectic solvent (DES) of choline chloride and urea with a molar ratio of 1:2 at different temperatures and interval times. The synthesised TiO2 was further utilised to produce diffracting TiO2 paste for application in dye-sensitised solar cells (DSCs) to improve the power conversation efficiency of devices. The TiO2 product analysed by X-ray diffraction (XRD) showed a similar phase and crystallinity to the raw material. However, scanning electron microscope (SEM) images showed that the hydrothermal TiO2 product had less particle aggregation than the raw one. The particle size was exceedingly 100 nm. The TiO2 pastes were prepared with a mixture of ethyl acetate and terpineol at volume ratios of 3:7 and 4:6. The paste was suitable to make thin films by the screen-printing method as a diffusion layer in cells. DSC devices using the TiO2 produced by the hydrothermal process with DES at 140oC for 18, 24, and 36 hours, respectively, enhanced the power conversion efficiency by 6.7-6.9%, which was higher than the ones using raw TiO2 by about 7%. Therefore, TiO2 powder modified by the hydrothermal process in DES solvent has great potential for application in DSC manufacture.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).38-44
Thi Diep Hoang, Thi Anh Duong Nguyen, Kien Thai Duong Nguyen, Duy Vu Nguyen, Thi Quynh Trang Luu, Thi Thu Phuong Tran, Minh Trien Pham
The rapid increase in fall armyworms (FAW, Spodoptera frugiperda) in recent years has posed major challenges to maize growers around the globe. To keep larval density below the economic threshold, we need interdisciplinary agricultural solutions like plant protection epidemiology, the Internet of Things, and scientific data techniques for early detection, monitoring, forecasting, and making informed decisions. Pest control should be planned ahead of time to prevent indiscriminate pesticide spraying, waste, and a negative effect on the environment. In this study, the authors plan to create a comprehensive iFAWcast software system that will automatically predict, alert, and gather research data on fall armyworms on maize crops in Vietnam. The system is comprised of three major components: (i) An automatic forecasting and alerting tool for fall armyworm outbreaks on the web platform; (ii) An agriculture reporting, forecasting, alerting, and user management tool on the web platform; and (iii) A mobile app that provides forecasting and alerting services on fall armyworms to farmers based on their geographical location. The iFAWcast system includes a central computation that dynamically updates weather forecasts from the Visual Crossing API and the OpenWeatherMap API, as well as a formula for the effective cumulative temperature based on the characteristics of fall armyworms on maize crops in Vietnam. The developed system was tested using data collected straight from the field, yielding extremely accurate and dependable results.
近年来，秋绵虫（FAW，Spodoptera frugiperda）的迅速增加给全球玉米种植者带来了重大挑战。为了将幼虫密度控制在经济阈值以下，我们需要跨学科的农业解决方案，如植物保护流行病学、物联网和科学数据技术，以实现早期检测、监测、预测和做出明智决策。病虫害防治应提前规划，以防止农药滥喷、浪费和对环境造成负面影响。在本研究中，作者计划创建一个综合 iFAWcast 软件系统，该系统将自动预测、预警并收集越南玉米作物上秋季军虫的研究数据。该系统由三个主要部分组成：(i) 网络平台上的自动预报和警报工具；(ii) 网络平台上的农业报告、预报、警报和用户管理工具；(iii) 移动应用程序，根据农民的地理位置向他们提供有关秋虫的预报和警报服务。iFAWcast 系统包括一个中央计算，可动态更新来自 Visual Crossing API 和 OpenWeatherMap API 的天气预报，以及一个基于越南玉米作物上秋季军团虫特征的有效积温公式。使用直接从田间收集的数据对所开发的系统进行了测试，结果非常准确可靠。
{"title":"Research and development of a predictive system for fall armyworm early warning on maize crop","authors":"Thi Diep Hoang, Thi Anh Duong Nguyen, Kien Thai Duong Nguyen, Duy Vu Nguyen, Thi Quynh Trang Luu, Thi Thu Phuong Tran, Minh Trien Pham","doi":"10.31276/vjst.66(3).38-44","DOIUrl":"https://doi.org/10.31276/vjst.66(3).38-44","url":null,"abstract":"The rapid increase in fall armyworms (FAW, Spodoptera frugiperda) in recent years has posed major challenges to maize growers around the globe. To keep larval density below the economic threshold, we need interdisciplinary agricultural solutions like plant protection epidemiology, the Internet of Things, and scientific data techniques for early detection, monitoring, forecasting, and making informed decisions. Pest control should be planned ahead of time to prevent indiscriminate pesticide spraying, waste, and a negative effect on the environment. In this study, the authors plan to create a comprehensive iFAWcast software system that will automatically predict, alert, and gather research data on fall armyworms on maize crops in Vietnam. The system is comprised of three major components: (i) An automatic forecasting and alerting tool for fall armyworm outbreaks on the web platform; (ii) An agriculture reporting, forecasting, alerting, and user management tool on the web platform; and (iii) A mobile app that provides forecasting and alerting services on fall armyworms to farmers based on their geographical location. The iFAWcast system includes a central computation that dynamically updates weather forecasts from the Visual Crossing API and the OpenWeatherMap API, as well as a formula for the effective cumulative temperature based on the characteristics of fall armyworms on maize crops in Vietnam. The developed system was tested using data collected straight from the field, yielding extremely accurate and dependable results.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).31-37
Duc Trung Tran, Thi Thu Hang Nguyen, Thu Huong Dao, Hong Linh Ta, Thi Lan Hoa Nguyen, Thanh Huyen Pham, Thi Hue Huong Tran, Hoang Nguyen
The chromosome-level genome assembly of passion fruit (Passiflora edulis Sims) has facilitated computational analyses in developing novel informative SSR markers for genetics and breeding research. In this study, in-silico analysis was conducted to discover potential microsatellite loci and to design specific primers from the sequences of the characterised nucleotide-binding site - leucine-rich repeats (NBS-LRR) gene family of passion fruit, and a genotyping experiment was performed to validate the novel simple sequence repeat (SSR) markers.In total, 497 microsatellite loci were detected from 161 passion fruit NBS-LRR genes, of which 383 loci were qualified for primer generation. Forty-six SSR markers were selected for polymorphism verification, targeting 35 NBS-LRR genes that showed potential gene expression in response to biotic and abiotic stresses. From that, 25 SSR markers showed consistent polymorphic alleles among different passion fruit genotypes via PCR-PAGE analysis. These novel NBS-LRR-specific SSR markers might provide valuable tools for fortifying research on germplasm evaluation, genetic mapping, and molecular breeding for disease-resistant passion fruit varieties.
百香果（Passiflora edulis Sims）染色体水平的基因组组装有助于进行计算分析，为遗传学和育种研究开发新的信息 SSR 标记。在这项研究中，研究人员从已鉴定的百香果核苷酸结合位点-富亮氨酸重复序列（NBS-LRR）基因家族的序列中，进行了发现潜在微卫星位点和设计特异性引物的海内分析，并进行了基因分型实验，以验证新型简单序列重复（SSR）标记。筛选出 46 个 SSR 标记进行多态性验证，目标是 35 个 NBS-LRR 基因，这些基因对生物和非生物胁迫有潜在的基因表达。通过 PCR-PAGE 分析，25 个 SSR 标记在不同百香果基因型中显示出一致的多态性等位基因。这些新型 NBS-LRR 特异性 SSR 标记可为加强抗病百香果品种的种质评估、基因图谱绘制和分子育种研究提供有价值的工具。
{"title":"Development of NBS-LRR gene-specific microsatellite markers for passion fruit Passiflora edulis Sims","authors":"Duc Trung Tran, Thi Thu Hang Nguyen, Thu Huong Dao, Hong Linh Ta, Thi Lan Hoa Nguyen, Thanh Huyen Pham, Thi Hue Huong Tran, Hoang Nguyen","doi":"10.31276/vjst.66(3).31-37","DOIUrl":"https://doi.org/10.31276/vjst.66(3).31-37","url":null,"abstract":"The chromosome-level genome assembly of passion fruit (Passiflora edulis Sims) has facilitated computational analyses in developing novel informative SSR markers for genetics and breeding research. In this study, in-silico analysis was conducted to discover potential microsatellite loci and to design specific primers from the sequences of the characterised nucleotide-binding site - leucine-rich repeats (NBS-LRR) gene family of passion fruit, and a genotyping experiment was performed to validate the novel simple sequence repeat (SSR) markers.In total, 497 microsatellite loci were detected from 161 passion fruit NBS-LRR genes, of which 383 loci were qualified for primer generation. Forty-six SSR markers were selected for polymorphism verification, targeting 35 NBS-LRR genes that showed potential gene expression in response to biotic and abiotic stresses. From that, 25 SSR markers showed consistent polymorphic alleles among different passion fruit genotypes via PCR-PAGE analysis. These novel NBS-LRR-specific SSR markers might provide valuable tools for fortifying research on germplasm evaluation, genetic mapping, and molecular breeding for disease-resistant passion fruit varieties.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.31276/vjst.66(3).01-05
Cong Cuong Do, D. L. Bui, Duc Ton Nguyen
This paper presents an overview of the experimental setup to study the structure of unstable nuclear isotopes in the region from 47Cl to 63V of the SEASTAR 3 project and the evolution of shell structure in neutron-rich nuclei N=32 and N=34. The first excited energies of Cl, Ar, and K isotopes around N=32, N=34 obtained from the SEASTAR 3 project were determined. The evolution of shell structure in neutron-rich nuclei N=32 và N=34 is explained by the contribution of tensor forces in addition to central and spin-orbit forces. The appearance of new magic neutron N=34 in 54Ca is explained due to proton-neutron tensor force that pulls neutron 0f5/2 lower than 1p1/2 level, leading to not only causes the spin inversion of neutrons at 1p1/2 and 0f5/2 levels and enlarges an energy gap between these levels. The change of proton and neutron energy levels in Cl, Ar, and K isotopes around N=32, N=34 driven by tensor force was also discussed in this article.
{"title":"Evolution of nuclear shell structure in neutron-rich nuclei N=32 and N=34","authors":"Cong Cuong Do, D. L. Bui, Duc Ton Nguyen","doi":"10.31276/vjst.66(3).01-05","DOIUrl":"https://doi.org/10.31276/vjst.66(3).01-05","url":null,"abstract":"This paper presents an overview of the experimental setup to study the structure of unstable nuclear isotopes in the region from 47Cl to 63V of the SEASTAR 3 project and the evolution of shell structure in neutron-rich nuclei N=32 and N=34. The first excited energies of Cl, Ar, and K isotopes around N=32, N=34 obtained from the SEASTAR 3 project were determined. The evolution of shell structure in neutron-rich nuclei N=32 và N=34 is explained by the contribution of tensor forces in addition to central and spin-orbit forces. The appearance of new magic neutron N=34 in 54Ca is explained due to proton-neutron tensor force that pulls neutron 0f5/2 lower than 1p1/2 level, leading to not only causes the spin inversion of neutrons at 1p1/2 and 0f5/2 levels and enlarges an energy gap between these levels. The change of proton and neutron energy levels in Cl, Ar, and K isotopes around N=32, N=34 driven by tensor force was also discussed in this article.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).90-95
N. Giang, Nguyen Trong Ha, Nguyen Thi Xuan
Lymphoma represents a heterogeneous group of cancers affecting the lymphatic system, encompassing non-Hodgkin lymphoma (NHL) and Hodgkin lymphoma (HL). The majority of NHL cases originate from B cells. HL is characterized by the presence of rare Hodgkin and Reed-Sternberg cells. Within the context of the inflammatory response, two SH2 domain-containing protein tyrosine phosphatases (SHPs), namely SHP-1 and SHP-2, serve as negative regulators. Deubiquitinating enzymes (DUBs) play a crucial role in inhibiting NF-κB activation in response to various stimuli. This study focuses on the DUBs OTUB1, OTUB2, and Cezanne. Blood samples were collected from 50 NHL patients, 26 HL patients, and 50 healthy individuals. Quantitative RT-PCR was employed to assess the mRNA expression of OTUB1, OTUB2, Cezanne, SHP-1,and SHP-2, while ELISA was used to determine IL-6 and CA125 concentrations. The results revealed that the mRNA level of OTUB1 was significantly downregulated in NHL patients but not in HL patients. Notably, Cezanne expression was downregulated in lymphoma patients, with significantly lower levels observed in HL compared to NHL patients. Furthermore, SHP-1 mRNA expression was significantly lower in the NHL group compared to the HL group or healthy individuals. Conversely, SHP-2 gene expression was upregulated in NHL patients but remained unchanged in HL patients. In conclusion, these findings highlight significant differences in the expressions of DUB and SHP genes and the inflammatory response in lymphoma patients. This study provides a foundation for further investigation into the roles of DUBs and tyrosine phosphatases in regulating the functional activation of lymphoma cells.
{"title":"Expression of several deubiquitinase and tyrosine phosphatase genes involved in inflammatory response in lymphoma","authors":"N. Giang, Nguyen Trong Ha, Nguyen Thi Xuan","doi":"10.31276/vjste.66(1).90-95","DOIUrl":"https://doi.org/10.31276/vjste.66(1).90-95","url":null,"abstract":"Lymphoma represents a heterogeneous group of cancers affecting the lymphatic system, encompassing non-Hodgkin lymphoma (NHL) and Hodgkin lymphoma (HL). The majority of NHL cases originate from B cells. HL is characterized by the presence of rare Hodgkin and Reed-Sternberg cells. Within the context of the inflammatory response, two SH2 domain-containing protein tyrosine phosphatases (SHPs), namely SHP-1 and SHP-2, serve as negative regulators. Deubiquitinating enzymes (DUBs) play a crucial role in inhibiting NF-κB activation in response to various stimuli. This study focuses on the DUBs OTUB1, OTUB2, and Cezanne. Blood samples were collected from 50 NHL patients, 26 HL patients, and 50 healthy individuals. Quantitative RT-PCR was employed to assess the mRNA expression of OTUB1, OTUB2, Cezanne, SHP-1,and SHP-2, while ELISA was used to determine IL-6 and CA125 concentrations. The results revealed that the mRNA level of OTUB1 was significantly downregulated in NHL patients but not in HL patients. Notably, Cezanne expression was downregulated in lymphoma patients, with significantly lower levels observed in HL compared to NHL patients. Furthermore, SHP-1 mRNA expression was significantly lower in the NHL group compared to the HL group or healthy individuals. Conversely, SHP-2 gene expression was upregulated in NHL patients but remained unchanged in HL patients. In conclusion, these findings highlight significant differences in the expressions of DUB and SHP genes and the inflammatory response in lymphoma patients. This study provides a foundation for further investigation into the roles of DUBs and tyrosine phosphatases in regulating the functional activation of lymphoma cells.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).53-58
Thanh Tung Truong, John Nielsen
Fluorine holds a prominent position within the realm of drug discovery and development, substantiated by its presence in approximately 25% of drugs approved by the US Food and Drug Administration (FDA). Consequently, the advancement of new fluorination reactions stands as a pivotal area in medicinal chemistry. In particular, the monofluoro-, difluoromethyl-, and trifluoromethyl- are three groups that appear most frequently in drug structure. Quinoline, owing to its privileged structural status, plays a crucial role in drug design and synthesis. Various approaches have been documented for the direct difluoromethylation of the C-2 and C-4 positions of the quinoline ring. However, achieving direct C-3 difluoromethylation has remained an elusive objective. In this study, we introduce a novel method for effecting the direct difluoromethylation at the C-3 position of the quinoline ring.Comprehensive characterizations, including 1H-NMR, 13C-NMR, and 19F-NMR for all compounds are performed. We believe that this novel method will open a new way to access the hitherto untapped C-3-difluoromethylation active compounds.
{"title":"Development of direct C-3 difluoromethylation reaction for application in synthesis of quinoline-related drugs","authors":"Thanh Tung Truong, John Nielsen","doi":"10.31276/vjste.66(1).53-58","DOIUrl":"https://doi.org/10.31276/vjste.66(1).53-58","url":null,"abstract":"Fluorine holds a prominent position within the realm of drug discovery and development, substantiated by its presence in approximately 25% of drugs approved by the US Food and Drug Administration (FDA). Consequently, the advancement of new fluorination reactions stands as a pivotal area in medicinal chemistry. In particular, the monofluoro-, difluoromethyl-, and trifluoromethyl- are three groups that appear most frequently in drug structure. Quinoline, owing to its privileged structural status, plays a crucial role in drug design and synthesis. Various approaches have been documented for the direct difluoromethylation of the C-2 and C-4 positions of the quinoline ring. However, achieving direct C-3 difluoromethylation has remained an elusive objective. In this study, we introduce a novel method for effecting the direct difluoromethylation at the C-3 position of the quinoline ring.Comprehensive characterizations, including 1H-NMR, 13C-NMR, and 19F-NMR for all compounds are performed. We believe that this novel method will open a new way to access the hitherto untapped C-3-difluoromethylation active compounds.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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