Determination of Antioxidant, Antimicrobial Activities, Total Phenolic and Flavonoid Contents of Allium rumelicum, Jurinea kilaea, Peucedanum obtusifolium

Abstract

A microwave-assisted extraction (MAE) process for polyphenols from Allium rumelicum Koçyigit & Özhatay, Jurinea kilaea Azn., Peucedanum obtusifolium Sibth. & Sm. was used. This research examined the methanolic extracts made from these three species' antioxidant, antimicrobial, total phenolic, and flavonoid contents. By using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical method (DPPH), ABTS/Persulfate, and Cupric reducing antioxidant capacity (CUPRAC) methods, the total antioxidant activities and capacities were examined. Additionally, the Folin-Ciocalteu and AlCl3/KAc techniques were used to calculate the total phenolic and flavonoid contents. In order to ascertain the antibacterial capabilities of plants, the disc diffusion method was applied. The Jurinea kilaea showed the greatest total antioxidant capacity/activity levels when measured using the CUPRAC and ABTS/Persulfate techniques. The Allium rumelicum was found to have the highest quercetin concentration, while Peucedanum obtusifolium had the lowest. In Jurinea kilaea, the gallic acid concentration was greatest.The highest antimicrobial activity values were obtained in Peucedanum obtusifolium