Modification Of Kaolin Capkala With Benzalkonium Chloride (Bkc) Surfactant And Antibacterial Activity Test Against Escherichia Coli

Jurnal Kimia Mulawarman Pub Date : 2023-11-22 DOI:10.30872/jkm.v21i1.1021

Jeny Clarita, P. Ardiningsih, Nelly Wahyuni

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Abstract

A study was conducted to determine the antibacterial activity of modified benzalkonium chloride (BKC) kaolin Capkala against Escherichia coli (E. coli) bacteria. Kaolin modification begins by melting kaolin with sodium hydroxide (NaOH) and reacting using a reflux device. Furthermore, the sample was reacted with BKC at a concentration variation of 0, 2.5×10-3; 5×10-3, and 2.5×10-2 M for 6 hours with a stirring speed of 150 rpm. The modified kaolin was characterized using Fourier Transformed Infrared (FTIR), and adsorption tests were performed on Cl-. The FTIR spectrum of the modified kaolin showed an absorption peak at 2926.19 cm-1 C-H symmetrical from CH2, which indicated the attachment of surfactant to the kaolin. BKC modified kaolin antibacterial activity test using the halo test method. The adsorption test on Cl- and the antibacterial activity test on kaolin and modified kaolin increased with increasing BKC concentration, namely 3545; 3828.6, 3970.4; 4183.1 mg/L and the inhibitory diameter were 2.1±0.14, 3.3±0.14, 4.5±0.14, and 6±0.14 mm, respectively. The results showed that BKC can increase the antibacterial activity of kaolin

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用苯扎氯铵（Bkc）表面活性剂改性 Capkala 高岭土并测试其对大肠杆菌的抗菌活性

本研究旨在确定改性苯扎氯铵（BKC）高岭土 Capkala 对大肠杆菌（E. coli）的抗菌活性。高岭土改性的第一步是用氢氧化钠（NaOH）熔化高岭土，并使用回流装置进行反应。然后，样品与浓度分别为 0、2.5×10-3、5×10-3 和 2.5×10-2 M 的 BKC 反应 6 小时，搅拌速度为 150 rpm。利用傅立叶变换红外光谱（FTIR）对改性高岭土进行了表征，并对 Cl- 进行了吸附测试。改性高岭土的傅立叶变换红外光谱在 2926.19 cm-1 处出现了一个与 CH2 对称的 C-H 吸收峰，表明表面活性剂附着在高岭土上。采用晕染试验法测试 BKC 改性高岭土的抗菌活性。随着 BKC 浓度的增加，对 Cl- 的吸附试验以及对高岭土和改性高岭土的抗菌活性试验均有所增加，分别为 3545、3828.6、3970.4 和 4183.1 mg/L，抑菌直径分别为 2.1±0.14、3.3±0.14、4.5±0.14 和 6±0.14 mm。结果表明，BKC 能提高高岭土的抗菌活性。

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Jurnal Kimia Mulawarman

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16 weeks