Anti-inflammatory activity test of extract and fraction of gaharu leaf (Aquilaria malaccensis Lamk.) against protein denaturation inhibition in vitro has been carried out. This study aims to determine the percent inhibition of protein denaturation and the strength of anti-inflammatory activity of the concentrated methanol extract, ethyl acetate fraction, and methanol-water fraction of gaharu leaves (Aquilaria malaccensis Lamk.). BSA (Bovine serum albumin) is used as the protein to be heated. Diclofenac sodium was used as the positive control, and the negative control used the appropriate solvent. Based on phytochemical screening tests, methanol extracts are known to contain secondary metabolites, including flavonoids, triterpenoids, steroids, and phenolics. The n-hexane fraction contains triterpenoid and steroid compounds. The ethyl acetate fraction contains flavonoid, triterpenoid, steroid, and phenolic compounds, while the methanol-water fraction contains flavonoid, triterpenoid and phenolic compounds. The results of the anti-inflammatory activity test of crude extract methanol and ethyl acetate fraction showed moderate antiinflammatory potential with IC50 values of 181.58- 168.87 ppm, respectively, while the methanol-water fraction has strong anti-inflammatory potential with IC50 value of 60.84 ppm.
{"title":"Anti-Inflammatory Effect Of Extract And Fractions Of Gaharu (Aquilaria malaccensis Lamk.) Leaves","authors":"Nur Hidayah, R. Ruga, Chairul Saleh","doi":"10.30872/jkm.v21i1.1022","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1022","url":null,"abstract":"Anti-inflammatory activity test of extract and fraction of gaharu leaf (Aquilaria malaccensis Lamk.) against protein denaturation inhibition in vitro has been carried out. This study aims to determine the percent inhibition of protein denaturation and the strength of anti-inflammatory activity of the concentrated methanol extract, ethyl acetate fraction, and methanol-water fraction of gaharu leaves (Aquilaria malaccensis Lamk.). BSA (Bovine serum albumin) is used as the protein to be heated. Diclofenac sodium was used as the positive control, and the negative control used the appropriate solvent. Based on phytochemical screening tests, methanol extracts are known to contain secondary metabolites, including flavonoids, triterpenoids, steroids, and phenolics. The n-hexane fraction contains triterpenoid and steroid compounds. The ethyl acetate fraction contains flavonoid, triterpenoid, steroid, and phenolic compounds, while the methanol-water fraction contains flavonoid, triterpenoid and phenolic compounds. The results of the anti-inflammatory activity test of crude extract methanol and ethyl acetate fraction showed moderate antiinflammatory potential with IC50 values of 181.58- 168.87 ppm, respectively, while the methanol-water fraction has strong anti-inflammatory potential with IC50 value of 60.84 ppm.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"47 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139205183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Siti Rusdiana Puspa Dewi, S. Sinulingga, Fatmawati Fatmawati, Maharani Natasya, Siti Devita Utami, Putra Ramadhan Algiffary Irianto Dunda
Ikan patin (Pangasius sp.) umumnya dijumpai pada perairan air tawar di Indonesia, seperti di Sumatera dan Kalimantan. Di Sumatera Selatan, ikan jenis ini digunakan sebagai salah satu kuliner seperti pindang dan brengkes. Tujuan dari penelitian ini adalah untuk mengetahui kandungan fitokimia metabolit sekunder dari ekstrak ikan patin (Pangasius sp.). Metode yang digunakan pada penelitian ini adalah studi deskriptif dengan menggunakan ekstrak yang diperoleh dari kulit, tulang, dan daging ikan patin. Ekstrak yang digunakan adalah ekstrak dari pelarut etanol, etil asetat, dan n-heksana dengan proses maserasi dan akudes dengan proses infusa. Hasil penelitian menunjukkan bahwa pada ekstrak etanol, kulit ikan mengandung alkaloid, saponin, dan steroid; pada tulang mengandung flavonoid dan saponin; pada daging mengandung alkaloid, saponin, dan flavonoid. Pada ekstrak etil asetat, kulit dan tulang ikan mengandung alkaloid; sedangkan pada daging mengandung flavonoid dan steroid. Pada ekstrak n-heksana, kulit ikan mengandung alkaloid dan steroid; tulang ikan mengandung alkaloid; dan daging ikan mengandung saponin dan terpenoid. Pada akuades, kulit ikan mengandung alkaloid, saponin, tanin, dan steroid; tulang ikan mengandung alkaloid; danging ikan mengandung alkaloid, flavoid, dan saponin. Kesimpulan dari penelitian ini bahwa Pangasius sp. memiliki metabolit sekunder alkaloid, flavonoid, saponin, tanin, terpenoid, dan steroid.
{"title":"Determination of Secondary Compounds in Extracts from Catfish (Pangasius sp)","authors":"Siti Rusdiana Puspa Dewi, S. Sinulingga, Fatmawati Fatmawati, Maharani Natasya, Siti Devita Utami, Putra Ramadhan Algiffary Irianto Dunda","doi":"10.30872/jkm.v21i1.1009","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1009","url":null,"abstract":"Ikan patin (Pangasius sp.) umumnya dijumpai pada perairan air tawar di Indonesia, seperti di Sumatera dan Kalimantan. Di Sumatera Selatan, ikan jenis ini digunakan sebagai salah satu kuliner seperti pindang dan brengkes. Tujuan dari penelitian ini adalah untuk mengetahui kandungan fitokimia metabolit sekunder dari ekstrak ikan patin (Pangasius sp.). Metode yang digunakan pada penelitian ini adalah studi deskriptif dengan menggunakan ekstrak yang diperoleh dari kulit, tulang, dan daging ikan patin. Ekstrak yang digunakan adalah ekstrak dari pelarut etanol, etil asetat, dan n-heksana dengan proses maserasi dan akudes dengan proses infusa. Hasil penelitian menunjukkan bahwa pada ekstrak etanol, kulit ikan mengandung alkaloid, saponin, dan steroid; pada tulang mengandung flavonoid dan saponin; pada daging mengandung alkaloid, saponin, dan flavonoid. Pada ekstrak etil asetat, kulit dan tulang ikan mengandung alkaloid; sedangkan pada daging mengandung flavonoid dan steroid. Pada ekstrak n-heksana, kulit ikan mengandung alkaloid dan steroid; tulang ikan mengandung alkaloid; dan daging ikan mengandung saponin dan terpenoid. Pada akuades, kulit ikan mengandung alkaloid, saponin, tanin, dan steroid; tulang ikan mengandung alkaloid; danging ikan mengandung alkaloid, flavoid, dan saponin. Kesimpulan dari penelitian ini bahwa Pangasius sp. memiliki metabolit sekunder alkaloid, flavonoid, saponin, tanin, terpenoid, dan steroid.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139207210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant activity test showed that the antioxidant activity of the ethyl acetate extract was higher (IC50=18.23μg/ml) than the n-butanol extract (IC50=29.58μg/ml). The results of the toxicity test on the ethyl acetate extract were higher (LC50=101.84 g/ml) than the n-butanol extract (LC50=154.59 g/ml). TLC analysis results showed that the mobile phase for the best separation of chemical compounds in ethyl acetate extract was chloroform-methanol (10:1) and n-butanol extract was the mobile phase chloroform-methanol-water (5:5:1), while HPLC analysis showed that the mobile phase for the separation of chemical compounds in the ethyl acetate extract was n-hexane-ethyl acetate (10:1) and the n-butanol extract was methanol-water (10:1).
{"title":"Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry","authors":"Vino Soaduon Hamonangan Simanjuntak, Endah Wydiastuti, Lina Marlina, Partomouan Simanjuntak, R. Kartika","doi":"10.30872/jkm.v21i1.1288","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1288","url":null,"abstract":"Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant activity test showed that the antioxidant activity of the ethyl acetate extract was higher (IC50=18.23μg/ml) than the n-butanol extract (IC50=29.58μg/ml). The results of the toxicity test on the ethyl acetate extract were higher (LC50=101.84 g/ml) than the n-butanol extract (LC50=154.59 g/ml). TLC analysis results showed that the mobile phase for the best separation of chemical compounds in ethyl acetate extract was chloroform-methanol (10:1) and n-butanol extract was the mobile phase chloroform-methanol-water (5:5:1), while HPLC analysis showed that the mobile phase for the separation of chemical compounds in the ethyl acetate extract was n-hexane-ethyl acetate (10:1) and the n-butanol extract was methanol-water (10:1).","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139198426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. M. Yuningsih, Dikdik Mulyadi, Siti Inayah, Sita Sopatul Marwah
Mother-in-law's tongue leaves (Sansevieria trifasciata P.) is a plant of the genus Sansevieria with flavonoid compound namely trifasciatin, as an antioxidant. Antioxidants can be used in cosmetics to protect the skin from free radicals. The purpose of this research was to analyze antioxidant activity of mother-in-law's tongue leaves extract and to obtain the best lotion formulation with various concentrations. Samples were extracted using 96% ethanol solvent with maceration method. Antioxidant activity was tested using DPPH method and intensity was measured at λ=517 nm by UV-Vis spectrophotometer. The IC50 value=49.72 ppm indicates a very strong antioxidant activity, compared to vitamin C=4.01 ppm (very strong). Extract was formulated in lotion with various concentrations of 0.5%, 1%, 3%, 6% and the comparison was vitamin C with same concentration as well as base without the addition of active substances. Based on observations for 4 weeks, 3% extract lotion is the best formulation. The organoleptic test results showed no change in shape, texture, color, odor, and pH so lotion was stable. In addition, the value of viscosity, spreadability, adhesion, irritation test according to established standards also has an antioxidant value 99.72% (strong category). So effective to ward off free radicals on the skin.
{"title":"Formulation And Physical Stability Test Of Mother-In-Law’s Tongue Leaves Extract Lotion As An Antioxidant","authors":"L. M. Yuningsih, Dikdik Mulyadi, Siti Inayah, Sita Sopatul Marwah","doi":"10.30872/jkm.v21i1.1235","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1235","url":null,"abstract":"Mother-in-law's tongue leaves (Sansevieria trifasciata P.) is a plant of the genus Sansevieria with flavonoid compound namely trifasciatin, as an antioxidant. Antioxidants can be used in cosmetics to protect the skin from free radicals. The purpose of this research was to analyze antioxidant activity of mother-in-law's tongue leaves extract and to obtain the best lotion formulation with various concentrations. Samples were extracted using 96% ethanol solvent with maceration method. Antioxidant activity was tested using DPPH method and intensity was measured at λ=517 nm by UV-Vis spectrophotometer. The IC50 value=49.72 ppm indicates a very strong antioxidant activity, compared to vitamin C=4.01 ppm (very strong). Extract was formulated in lotion with various concentrations of 0.5%, 1%, 3%, 6% and the comparison was vitamin C with same concentration as well as base without the addition of active substances. Based on observations for 4 weeks, 3% extract lotion is the best formulation. The organoleptic test results showed no change in shape, texture, color, odor, and pH so lotion was stable. In addition, the value of viscosity, spreadability, adhesion, irritation test according to established standards also has an antioxidant value 99.72% (strong category). So effective to ward off free radicals on the skin.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139235380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hypertension is a structural or functional change in the arteries or the organs it supplies caused by increased blood pressure. Angiotensin-converting enzyme (ACE) can increase blood pressure by converting inactive angiotensin I to active (angiotensin II). Captopril is a hypertension drug that can inhibit ACE activity. Sembung leaf (Blumea balsamifera DC) is a plant that can potentially have antihypertensive activity. This study aims to identify the interaction of active compounds in sembung leaves against ACE as antihypertensive drug candidates through an in silico test based on pharmacodynamic and pharmacokinetic parameters using two docking software, Autodock Vina and PyRx. The results showed that Luteolin was the best test ligand besides having lower ΔG and Ki than control ligands and higher Kd than control ligands. Luteolin was identified to interact with Zn and hydrogen bond interactions at the active site and met the criteria in Lipinski analysis, ADME, and toxicity, so this compound is relatively safe to be used as a drug candidate for the treatment of hypertension.
{"title":"Active Compounds of Sembung Leaves (Blumea balsamifera DC) in Silico Screening as Antihypertensives","authors":"Ahmad Sjahriza, Fyrda Shellia, Dyah Iswantini","doi":"10.30872/jkm.v21i1.1263","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1263","url":null,"abstract":"Hypertension is a structural or functional change in the arteries or the organs it supplies caused by increased blood pressure. Angiotensin-converting enzyme (ACE) can increase blood pressure by converting inactive angiotensin I to active (angiotensin II). Captopril is a hypertension drug that can inhibit ACE activity. Sembung leaf (Blumea balsamifera DC) is a plant that can potentially have antihypertensive activity. This study aims to identify the interaction of active compounds in sembung leaves against ACE as antihypertensive drug candidates through an in silico test based on pharmacodynamic and pharmacokinetic parameters using two docking software, Autodock Vina and PyRx. The results showed that Luteolin was the best test ligand besides having lower ΔG and Ki than control ligands and higher Kd than control ligands. Luteolin was identified to interact with Zn and hydrogen bond interactions at the active site and met the criteria in Lipinski analysis, ADME, and toxicity, so this compound is relatively safe to be used as a drug candidate for the treatment of hypertension.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139236422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Teguh Wirawan, Ishak Wiradikara Az, Noor Hindryawati
Research on the adsorption of methylene blue by activated charcoal from Empty Fruit Bunch (EFB) has been carried out. This study aimed to synthesize activated charcoal from EFB, characterize it, and use it as an adsorbent for methylene blue. Activated charcoal was synthesized by carbonation in a furnace at 300oC for 1 hour. Chemically, activation was carried out by immersion in HCl solution for 24 hours. Characterization was carried out by proximate test, determination of functional groups using Fourier Transform Infrared (FT-IR), and determining material shape with X-Ray Diffraction (XRD). The study of activated charcoal adsorption as methylene blue adsorbent was carried out by treating variations in contact time, adsorbent weight, and concentration. The results of the proximate test with the parameters of surface area, moisture content, and ash content were 162.12 m2/g, 0.52%, and 5.27%, respectively. The results of the FT-IR characterization test showed an aromatic C–H, C=C and C–O. Based on the results of characterization with XRD giving a pattern at a diffraction angle of 2 20 - 30o, it was seen that there was a wide peak, which indicated that it had an amorphous structure. The optimum conditions for methylene blue adsorption occurred at a contact time of 40 minutes, and the adsorbent weight was 0.1 g for 10 mL of 50 ppm methylene blue. The adsorption of methylene blue followed the Freundlich isotherm adsorption pattern and had a maximum adsorption capacity of 8.92 mg/g.
{"title":"Adsorption Of Methylene Blue Using Active Charcoal From Empty Fruit Bunch (EFB)","authors":"Teguh Wirawan, Ishak Wiradikara Az, Noor Hindryawati","doi":"10.30872/jkm.v21i1.1013","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1013","url":null,"abstract":"Research on the adsorption of methylene blue by activated charcoal from Empty Fruit Bunch (EFB) has been carried out. This study aimed to synthesize activated charcoal from EFB, characterize it, and use it as an adsorbent for methylene blue. Activated charcoal was synthesized by carbonation in a furnace at 300oC for 1 hour. Chemically, activation was carried out by immersion in HCl solution for 24 hours. Characterization was carried out by proximate test, determination of functional groups using Fourier Transform Infrared (FT-IR), and determining material shape with X-Ray Diffraction (XRD). The study of activated charcoal adsorption as methylene blue adsorbent was carried out by treating variations in contact time, adsorbent weight, and concentration. The results of the proximate test with the parameters of surface area, moisture content, and ash content were 162.12 m2/g, 0.52%, and 5.27%, respectively. The results of the FT-IR characterization test showed an aromatic C–H, C=C and C–O. Based on the results of characterization with XRD giving a pattern at a diffraction angle of 2 20 - 30o, it was seen that there was a wide peak, which indicated that it had an amorphous structure. The optimum conditions for methylene blue adsorption occurred at a contact time of 40 minutes, and the adsorbent weight was 0.1 g for 10 mL of 50 ppm methylene blue. The adsorption of methylene blue followed the Freundlich isotherm adsorption pattern and had a maximum adsorption capacity of 8.92 mg/g.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"39 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A study was conducted to determine the antibacterial activity of modified benzalkonium chloride (BKC) kaolin Capkala against Escherichia coli (E. coli) bacteria. Kaolin modification begins by melting kaolin with sodium hydroxide (NaOH) and reacting using a reflux device. Furthermore, the sample was reacted with BKC at a concentration variation of 0, 2.5×10-3; 5×10-3, and 2.5×10-2 M for 6 hours with a stirring speed of 150 rpm. The modified kaolin was characterized using Fourier Transformed Infrared (FTIR), and adsorption tests were performed on Cl-. The FTIR spectrum of the modified kaolin showed an absorption peak at 2926.19 cm-1 C-H symmetrical from CH2, which indicated the attachment of surfactant to the kaolin. BKC modified kaolin antibacterial activity test using the halo test method. The adsorption test on Cl- and the antibacterial activity test on kaolin and modified kaolin increased with increasing BKC concentration, namely 3545; 3828.6, 3970.4; 4183.1 mg/L and the inhibitory diameter were 2.1±0.14, 3.3±0.14, 4.5±0.14, and 6±0.14 mm, respectively. The results showed that BKC can increase the antibacterial activity of kaolin
{"title":"Modification Of Kaolin Capkala With Benzalkonium Chloride (Bkc) Surfactant And Antibacterial Activity Test Against Escherichia Coli","authors":"Jeny Clarita, P. Ardiningsih, Nelly Wahyuni","doi":"10.30872/jkm.v21i1.1021","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1021","url":null,"abstract":"A study was conducted to determine the antibacterial activity of modified benzalkonium chloride (BKC) kaolin Capkala against Escherichia coli (E. coli) bacteria. Kaolin modification begins by melting kaolin with sodium hydroxide (NaOH) and reacting using a reflux device. Furthermore, the sample was reacted with BKC at a concentration variation of 0, 2.5×10-3; 5×10-3, and 2.5×10-2 M for 6 hours with a stirring speed of 150 rpm. The modified kaolin was characterized using Fourier Transformed Infrared (FTIR), and adsorption tests were performed on Cl-. The FTIR spectrum of the modified kaolin showed an absorption peak at 2926.19 cm-1 C-H symmetrical from CH2, which indicated the attachment of surfactant to the kaolin. BKC modified kaolin antibacterial activity test using the halo test method. The adsorption test on Cl- and the antibacterial activity test on kaolin and modified kaolin increased with increasing BKC concentration, namely 3545; 3828.6, 3970.4; 4183.1 mg/L and the inhibitory diameter were 2.1±0.14, 3.3±0.14, 4.5±0.14, and 6±0.14 mm, respectively. The results showed that BKC can increase the antibacterial activity of kaolin","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"365 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of plants as a source of medicine has been increasing lately. The plants used generally have bioactivities such as anti-bacterial, antioxidant, and others. Plants that contain antioxidants are widely used because of their ability to capture free radicals so that they can protect the body from various diseases. One of the plants that is often used is plants from the Malvaceae family. In this study, the antioxidant and total phenolic contents of five types of plants were determined. These plants were extracted by infundation method and analyzed using MPM (Modified Phenanthroline Method) and Folin-Ciocalteu methods. The highest antioxidant and total phenolic contents were found in kapok randu and waru leaves.
{"title":"Determination of Total Antioxidant and Phenolic Content from Malvaceae Family Leaves Infusion by Spectrophotometry","authors":"Yefrida Yefrida, Silvia Detri Susanti, Refilda Refilda","doi":"10.30872/jkm.v21i1.1007","DOIUrl":"https://doi.org/10.30872/jkm.v21i1.1007","url":null,"abstract":"The use of plants as a source of medicine has been increasing lately. The plants used generally have bioactivities such as anti-bacterial, antioxidant, and others. Plants that contain antioxidants are widely used because of their ability to capture free radicals so that they can protect the body from various diseases. One of the plants that is often used is plants from the Malvaceae family. In this study, the antioxidant and total phenolic contents of five types of plants were determined. These plants were extracted by infundation method and analyzed using MPM (Modified Phenanthroline Method) and Folin-Ciocalteu methods. The highest antioxidant and total phenolic contents were found in kapok randu and waru leaves.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"43 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139248323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Analisa chlorampenicol pada udang windu secara spektrofotometri berbasis reaksi diazotasi telah dilakukan. sulfanilamid digunakan sebagai sumber garam diazo yang akan dikopling dengan chlorampenicol. Pada penelitian ini dilakukan beberapa optimasi seperti optimasi suhu, volume HCl, volume NaNO2, volume sulfanilamid dan waktu kopling. Senyawa yang dihasilkan yaitu senyawa azo sulfanilamid-chlorampenicol berwarna kuning jernih yang akan diukur dengan spektrofotometri visible. Dilakukan aplikasi pada sampel udang windu yang telah dipreparasi. Berdasarkan hasil pengujian chlorampenicol pada sampel udang windu, diperoleh nilai koefisien variasi pada konsentrasi chlorampenicol 20 µg/mL, 40 µg/mL dan 70 µg/mL berturut turut sebesar 4,0269 %, 1,9509 % dan 4,2771 %. dengan rata rata sebesar 3,4183 % lebih kecil 1/8,5 dibandingkan CV Horwitz sebesar 9,2777%. Nilai akurasi pada rentang 99,7143% sampai 100,8730%. Nilai limit deteksi sebesar 6,2411 mg/mL dan nilai limit kuantisasi sebesar 20,8036 mg/mL. Serta Nilai sensitivitas sebesar 0,0021 mL/mg.
{"title":"SPECTROPHOTOMETRIC ANALYSIS OF CHLORAMPENICOL IN TIGER PRAWNS (PENAEUS MONODON) BASED ON THE DIAZOTATION REACTION","authors":"Indra Kurniawan, M. Arif, Bohari Yusuf","doi":"10.30872/jkm.v20i2.1017","DOIUrl":"https://doi.org/10.30872/jkm.v20i2.1017","url":null,"abstract":"Analisa chlorampenicol pada udang windu secara spektrofotometri berbasis reaksi diazotasi telah dilakukan. sulfanilamid digunakan sebagai sumber garam diazo yang akan dikopling dengan chlorampenicol. Pada penelitian ini dilakukan beberapa optimasi seperti optimasi suhu, volume HCl, volume NaNO2, volume sulfanilamid dan waktu kopling. Senyawa yang dihasilkan yaitu senyawa azo sulfanilamid-chlorampenicol berwarna kuning jernih yang akan diukur dengan spektrofotometri visible. Dilakukan aplikasi pada sampel udang windu yang telah dipreparasi. Berdasarkan hasil pengujian chlorampenicol pada sampel udang windu, diperoleh nilai koefisien variasi pada konsentrasi chlorampenicol 20 µg/mL, 40 µg/mL dan 70 µg/mL berturut turut sebesar 4,0269 %, 1,9509 % dan 4,2771 %. dengan rata rata sebesar 3,4183 % lebih kecil 1/8,5 dibandingkan CV Horwitz sebesar 9,2777%. Nilai akurasi pada rentang 99,7143% sampai 100,8730%. Nilai limit deteksi sebesar 6,2411 mg/mL dan nilai limit kuantisasi sebesar 20,8036 mg/mL. Serta Nilai sensitivitas sebesar 0,0021 mL/mg.","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42961564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sintesis dietanolamida dari metil ester minyak biji wijen (Sesamum indicum L.) telah dilakukan dengan tahapan meliputi proses ekstraksi, transesterifikasi dan amidasi. Hasil penelitian menunjukkan pada proses ekstraksi secara maserasi diperoleh rendemen minyak biji wijen sebesar 36,14% dengan bilangan asam sebesar 1,1531 mg KOH/g, kadar ALB sebesar 0,5796% dan bilangan penyabunan sebesar 51,287 mg KOH/g. Pada proses konversi minyak biji wijen menjadi metil ester melalui transesterifikasi diperoleh bilangan asam sebesar 0,5126 mg KOH/g, kadar ALB turun menjadi 0,2704% dan bilangan penyabunan sebesar 50,049 mg KOH/g. Berdasarkan analisa GC-MS metil ester, komposisi yang paling dominan adalah metil oleat sebesar 39,13%. Berdasarkan spektrum FT-IR metil ester memberikan puncak serapan yang spesifik pada gugus C=O ester dengan bilangan gelombang 1743 cm-1. Pada proses konversi metil ester menjadi dietanolamida melalui proses amidasi menghasilkan nilai bilangan asam sebesar 0,3559 mg KOH/g, kadar ALB turun menjadi 0,2341% dan bilangan penyabunan sebesar 0,2749 mg KOH/g. Berdasarkan spektrum FT-IR dietanolamida memberikan puncak serapan spesifik untuk gugus C=O amida pada bilangan gelombang 1651 cm-1 dan C-N pada bilangan gelombang 1056 cm-1. Nilai HLB dietanolamida secara praktik sebesar 4,552 yang termasuk surfaktan golongan pengemulsi water in oil (W/O).
{"title":"KARAKTERISASI SIFAT FISIK KIMIA METIL ESTER MINYAK BIJI WIJEN (Sesamum indicum L.) DAN KOMPOSISINYA","authors":"Aji Zaldya Nmc, Daniel Daniel, I. A. Hiyahara","doi":"10.30872/jkm.v20i2.946","DOIUrl":"https://doi.org/10.30872/jkm.v20i2.946","url":null,"abstract":"Sintesis dietanolamida dari metil ester minyak biji wijen (Sesamum indicum L.) telah dilakukan dengan tahapan meliputi proses ekstraksi, transesterifikasi dan amidasi. Hasil penelitian menunjukkan pada proses ekstraksi secara maserasi diperoleh rendemen minyak biji wijen sebesar 36,14% dengan bilangan asam sebesar 1,1531 mg KOH/g, kadar ALB sebesar 0,5796% dan bilangan penyabunan sebesar 51,287 mg KOH/g. Pada proses konversi minyak biji wijen menjadi metil ester melalui transesterifikasi diperoleh bilangan asam sebesar 0,5126 mg KOH/g, kadar ALB turun menjadi 0,2704% dan bilangan penyabunan sebesar 50,049 mg KOH/g. Berdasarkan analisa GC-MS metil ester, komposisi yang paling dominan adalah metil oleat sebesar 39,13%. Berdasarkan spektrum FT-IR metil ester memberikan puncak serapan yang spesifik pada gugus C=O ester dengan bilangan gelombang 1743 cm-1. Pada proses konversi metil ester menjadi dietanolamida melalui proses amidasi menghasilkan nilai bilangan asam sebesar 0,3559 mg KOH/g, kadar ALB turun menjadi 0,2341% dan bilangan penyabunan sebesar 0,2749 mg KOH/g. Berdasarkan spektrum FT-IR dietanolamida memberikan puncak serapan spesifik untuk gugus C=O amida pada bilangan gelombang 1651 cm-1 dan C-N pada bilangan gelombang 1056 cm-1. Nilai HLB dietanolamida secara praktik sebesar 4,552 yang termasuk surfaktan golongan pengemulsi water in oil (W/O).","PeriodicalId":31725,"journal":{"name":"Jurnal Kimia Mulawarman","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43448304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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