B-2 lymphocytes and the balance of pro and anti-inflammatory cytokines in infectious and autoimmune phenotypes of common variable immune deficiency

Abstract

Objective: comparative characteristics of the subpopulation composition of B- lymphocytes and the cytokine spectrum of peripheral blood in infectious and non-infectious manifestations of CVID.Materials and methods: the results of observation of 10 people diagnosed with CVID have been presented. Six patients the clinical manifestation had an infectious phenotype of the disease of synopulmonary localization. Four patients were diagnosed with Crohn's disease, hemolytic anemia, and autoimmune hepatitis. The level of cytokines IL-4, IL-10, IL-17, TNF-α, IFN-γ in blood serum was determined by the ELISA, the phenotypic characteristic of B cells of peripheral blood cells was carried out by flow cytofluorimetry.Results: the proportion of memory B cells in the healthy donors is 30% of all B-lymphocytes, on infectious manifestation of CVID – 12%, with autoimmune – 14%. The number of switched memory B-lymphocytes relative to the total pool of B-2 cells in infectious manifestation is greater (2.3%) than in autoimmune ones (1.4%). In patients with infectious manifestations the content of IFN-γ and TNF-α was increased inrelation to healthy donors of the comparison group, there were no differences in the serum amount of IL-17, with autoimmune manifestations, the content of all these cytokines was increased, IFN-γ and TNF-α being to a greater extent.Conclusion: impaired formation of memory B-lymphocytes and cytokine dysregulation of immune processes are detected regardless of the variant of clinical manifestation of CVID. The nature and degree of changes differ depending on the clinical phenotype of the disease.