Pub Date : 2023-12-20DOI: 10.21886/2219-8075-2023-14-4-77-82
M. R. Kantsurova, A. N. Rymashevsky, V. V. Voloshin, N. Sapronova, S. S. Todorov, M. A. Rymashevsky, R. N. Kantsurov
Objective: to study in an experiment on animals pathomorphological changes in the tissues of the uterus, appendages and blood vessels after ligation of the internal iliac and ovarian arteries.Materials and methods: An experiment was conducted with 6 mature pigs. Group I (n=3) consisted of intact pigs without surgery, group II (n=3) included animals with ligation of the internal iliac and ovarian arteries on both sides. 12 months after the operation, slaughter was performed and the organo-complex of the uterus with appendages and iliac vessels was taken for electron microscopic pathomorphological examination.Results: comparative results of the study of the uterus, appendages found that the normal structure of the mature uterus was preserved, there were no changes in the structure of the fallopian tubes, tissues and vessels of the ovaries. All changes were found in the area of ligation of the ovarian and internal iliac arteries, although the arteries retained their lumen, but were deformed and surrounded by dense fibrous connective tissue. Conclusion: ligation of the ovarian and internal iliac arteries with absorbable suture material causes only local changes. The sufficiency of collateral blood flow is confirmed by the absence of atrophy of the uterus, fallopian tubes and ovaries, as well as organ deformities.
{"title":"Results of ligation the internal iliac and ovarian arteries in the experiment","authors":"M. R. Kantsurova, A. N. Rymashevsky, V. V. Voloshin, N. Sapronova, S. S. Todorov, M. A. Rymashevsky, R. N. Kantsurov","doi":"10.21886/2219-8075-2023-14-4-77-82","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-77-82","url":null,"abstract":"Objective: to study in an experiment on animals pathomorphological changes in the tissues of the uterus, appendages and blood vessels after ligation of the internal iliac and ovarian arteries.Materials and methods: An experiment was conducted with 6 mature pigs. Group I (n=3) consisted of intact pigs without surgery, group II (n=3) included animals with ligation of the internal iliac and ovarian arteries on both sides. 12 months after the operation, slaughter was performed and the organo-complex of the uterus with appendages and iliac vessels was taken for electron microscopic pathomorphological examination.Results: comparative results of the study of the uterus, appendages found that the normal structure of the mature uterus was preserved, there were no changes in the structure of the fallopian tubes, tissues and vessels of the ovaries. All changes were found in the area of ligation of the ovarian and internal iliac arteries, although the arteries retained their lumen, but were deformed and surrounded by dense fibrous connective tissue. Conclusion: ligation of the ovarian and internal iliac arteries with absorbable suture material causes only local changes. The sufficiency of collateral blood flow is confirmed by the absence of atrophy of the uterus, fallopian tubes and ovaries, as well as organ deformities.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"11 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.21886/2219-8075-2023-14-4-89-95
S. A. Tyo, O. S. Turkina
Hypospadias is one of the most common congenital malformations of the male external genitalia and urethra, the main manifestation of which is meatus dystopia. There is a steady increase in the frequency of its occurrence in many countries of the world. Hypospadias have various forms of severity, which significantly affects the patient's management tactics, the volume of surgical treatment and the further prognosis for the boy's life. The article describes a case of early prenatal diagnosis of the coronary form of hypospadias in the fetus at 15 weeks of gestation, followed by postnatal verification of the diagnosis. The possibilities of echography in the early detection of distal forms of hypospadias using an integrated approach in the assessment of external genitalia in a male fetus are shown. This complex of hypospadias markers includes an assessment of the size of the penis, the presence of a curvature of its body, the shape of the head of the penis and the presence of signs of cleavage of the foreskin, which are manifested by its rounding. The ineffectiveness of the long-used «tulip» sign for the early diagnosis of distal forms of hypospadias has been demonstrated. The comparison of the echographic picture of the structure of the genitals in normal and hypospadias at 12 and 15 weeks of pregnancy was carried out.
{"title":"Clinical observation of early prenatal diagnosis of the coronary of hypospadias","authors":"S. A. Tyo, O. S. Turkina","doi":"10.21886/2219-8075-2023-14-4-89-95","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-89-95","url":null,"abstract":"Hypospadias is one of the most common congenital malformations of the male external genitalia and urethra, the main manifestation of which is meatus dystopia. There is a steady increase in the frequency of its occurrence in many countries of the world. Hypospadias have various forms of severity, which significantly affects the patient's management tactics, the volume of surgical treatment and the further prognosis for the boy's life. The article describes a case of early prenatal diagnosis of the coronary form of hypospadias in the fetus at 15 weeks of gestation, followed by postnatal verification of the diagnosis. The possibilities of echography in the early detection of distal forms of hypospadias using an integrated approach in the assessment of external genitalia in a male fetus are shown. This complex of hypospadias markers includes an assessment of the size of the penis, the presence of a curvature of its body, the shape of the head of the penis and the presence of signs of cleavage of the foreskin, which are manifested by its rounding. The ineffectiveness of the long-used «tulip» sign for the early diagnosis of distal forms of hypospadias has been demonstrated. The comparison of the echographic picture of the structure of the genitals in normal and hypospadias at 12 and 15 weeks of pregnancy was carried out.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"56 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139169900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-19DOI: 10.21886/2219-8075-2023-14-4-44-57
A. A. Khamatova, I. Balmasova, T. A. Chebotareva
Objective: to investigate single nucleotide polymorphisms in IFNγ gene variant rs2430561 and in NKG2D gene variant rs2617160 as potential risk factors for mother-to-child viral transmission among HIV/HCV-coinfected women.Materials and methods: 65 mother-child pairs were examined, divided into 4 groups depending on the child's infection (HIV/ HCV infected, HIV or HCV infected, not infected). Methods: multiplex polymerase chain reaction (PCR), PCR for determining the viral load of HIV and HCV, flow cytometry for immunological studies, statistical analysis.Results: the single nucleotide polymorphism in IFNγ gene variant rs2430561 had no prognostic value when determined in the mothers. When recording the TT and AT genotypes of a single nucleotide polymorphism in this gene in a child, it was combined with the probability of the child's infection with HIV/HCV, HIV or HCV, depending on the magnitude of the viral load of HIV and HCV in the mother's blood in different trimesters of pregnancy. The presence of the AA and AT genotypes of the NKG2D gene variant rs2617160 in the mothers had a prognostic value as contributing tothe child infection with HIV/HCV or HIV alone. It was noted that the mechanisms of perinatal viral transmission included a certain level of decline in the absolute number of CD4+ lymphocytes in the woman blood in the second and third trimesters of pregnancy.Conclusions: the presence of AA and AT genotypes of the NKG2D gene variant rs2617160 in woman co-infected with HIV/HCV makes it possible to predict the risk of child infection not only during pregnancy, but also at the planning stage.
目的：研究IFNγ基因变异体rs2430561和NKG2D基因变异体rs2617160的单核苷酸多态性作为HIV/HCV共感染妇女母婴病毒传播的潜在危险因素。材料和方法：研究对象为65对母婴，根据儿童感染情况分为4组（HIV/HCV感染、HIV或HCV感染、未感染）。方法：多重聚合酶链反应（PCR）、用于确定 HIV 和 HCV 病毒载量的 PCR、用于免疫学研究的流式细胞术、统计分析。结果：IFNγ 基因变异体 rs2430561 的单核苷酸多态性在确定母亲时没有预后价值。在记录孩子体内该基因单核苷酸多态性的 TT 和 AT 基因型时，根据怀孕不同孕期母亲血液中 HIV 和 HCV 病毒载量的大小，结合孩子感染 HIV/HCV、HIV 或 HCV 的概率。母亲体内存在的 NKG2D 基因变异体 rs2617160 的 AA 和 AT 基因型对儿童感染 HIV/HCV 或仅感染 HIV 有预后价值。结论：合并感染艾滋病毒/HCV 的妇女体内存在 NKG2D 基因变异体 rs2617160 的 AA 和 AT 基因型，不仅可以在孕期预测儿童感染的风险，还可以在计划阶段预测儿童感染的风险。
{"title":"Polymorphisms of interferon γ and NKG2D receptor genes in predicting vertical transmission of HIV/HCV coinfection","authors":"A. A. Khamatova, I. Balmasova, T. A. Chebotareva","doi":"10.21886/2219-8075-2023-14-4-44-57","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-44-57","url":null,"abstract":"Objective: to investigate single nucleotide polymorphisms in IFNγ gene variant rs2430561 and in NKG2D gene variant rs2617160 as potential risk factors for mother-to-child viral transmission among HIV/HCV-coinfected women.Materials and methods: 65 mother-child pairs were examined, divided into 4 groups depending on the child's infection (HIV/ HCV infected, HIV or HCV infected, not infected). Methods: multiplex polymerase chain reaction (PCR), PCR for determining the viral load of HIV and HCV, flow cytometry for immunological studies, statistical analysis.Results: the single nucleotide polymorphism in IFNγ gene variant rs2430561 had no prognostic value when determined in the mothers. When recording the TT and AT genotypes of a single nucleotide polymorphism in this gene in a child, it was combined with the probability of the child's infection with HIV/HCV, HIV or HCV, depending on the magnitude of the viral load of HIV and HCV in the mother's blood in different trimesters of pregnancy. The presence of the AA and AT genotypes of the NKG2D gene variant rs2617160 in the mothers had a prognostic value as contributing tothe child infection with HIV/HCV or HIV alone. It was noted that the mechanisms of perinatal viral transmission included a certain level of decline in the absolute number of CD4+ lymphocytes in the woman blood in the second and third trimesters of pregnancy.Conclusions: the presence of AA and AT genotypes of the NKG2D gene variant rs2617160 in woman co-infected with HIV/HCV makes it possible to predict the risk of child infection not only during pregnancy, but also at the planning stage.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":" 791","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138960410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-11DOI: 10.21886/2219-8075-2023-14-4-29-34
R. T. Urazmambetov, S. A. Chebotov, T. V. Loboda
Objective: comparative characteristics of indicators of psycho-emotional and immune statuses of first-year students in various conditions of the educational process.Materials and methods: 2nd groups of first-year students of Rostov State Medical University from the medical and preventive faculty (18 people) and the military training center (17 people) were examined. They were all comparable in age (19 ±1 and 19±2 years), gender (all men), physical condition (corresponded to the I-st health group). The immune status was assessed at the Research Institute of Clinical Immunology using standard methodological approaches. Psychological testing was carried out using the Spielberger-Khanin scale, A. Beck's depression diagnostic methodology, and K. Rogers and R. Diamond questionnaire.Results: the differences in the immune response system between the compared groups were revealed only in relation to the parameters characterizing innate immunity. In relation to Medical and Preventive Faculty students, the functional capabilities of natural killers in Military Training Centre students are reduced and the expression of one of the most indicative pattern-recognizing receptors by monocytes is diminished. The analysis of the obtained data of the psychological questionnaire showed that the students of the Military Training Centredemonstrate a lower degree of situational and personal anxiety with a lower degree of adaptation in comparison with the first-year students of the Medical and Preventive Faculty.Conclusion: the immune system indicators responsible for the processes of primary immune response are reduced in military medical students comparing with medical students, which in its turn forms possible prerequisites for the development of clinical manifestations of immune dysfunction. The period of psychological adaptation to the educational process in a medical university is less successful for first-year students of the Military Training Centre. It is necessary to develop measures aimed at increasing the sense of responsibility, psychoemotional and immune adaptation resources.
{"title":"Features of both immune and psycho-emotional factors in the adaptive reactions of first-year students","authors":"R. T. Urazmambetov, S. A. Chebotov, T. V. Loboda","doi":"10.21886/2219-8075-2023-14-4-29-34","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-29-34","url":null,"abstract":"Objective: comparative characteristics of indicators of psycho-emotional and immune statuses of first-year students in various conditions of the educational process.Materials and methods: 2nd groups of first-year students of Rostov State Medical University from the medical and preventive faculty (18 people) and the military training center (17 people) were examined. They were all comparable in age (19 ±1 and 19±2 years), gender (all men), physical condition (corresponded to the I-st health group). The immune status was assessed at the Research Institute of Clinical Immunology using standard methodological approaches. Psychological testing was carried out using the Spielberger-Khanin scale, A. Beck's depression diagnostic methodology, and K. Rogers and R. Diamond questionnaire.Results: the differences in the immune response system between the compared groups were revealed only in relation to the parameters characterizing innate immunity. In relation to Medical and Preventive Faculty students, the functional capabilities of natural killers in Military Training Centre students are reduced and the expression of one of the most indicative pattern-recognizing receptors by monocytes is diminished. The analysis of the obtained data of the psychological questionnaire showed that the students of the Military Training Centredemonstrate a lower degree of situational and personal anxiety with a lower degree of adaptation in comparison with the first-year students of the Medical and Preventive Faculty.Conclusion: the immune system indicators responsible for the processes of primary immune response are reduced in military medical students comparing with medical students, which in its turn forms possible prerequisites for the development of clinical manifestations of immune dysfunction. The period of psychological adaptation to the educational process in a medical university is less successful for first-year students of the Military Training Centre. It is necessary to develop measures aimed at increasing the sense of responsibility, psychoemotional and immune adaptation resources.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"5 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138980317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-04DOI: 10.21886/2219-8075-2023-14-4-114-121
A. M. Levchin, A. Lebedenko, I. B. Ershova, A. G. Rogovtsova
Objective: to study the indicators of the functional state of the body and working capacity in the dynamics of learning in elementary school children with different school success.Materials and methods: the study included 573 elementary school students. Group I – 82 students with excellent academic performance Group II – 491 children with good academic performance. The vegetative index of Kerdo (VIC), the Rufier test, the Genchi test, and the adaptive index (AP) were determined by the method of F. Halberg. The mental performance of younger schoolchildren was studied using Schulte tables.Results: the values of VIC in group I children were 2.5–3.5 times higher. The difference in IR between the groups increased, amounting to 32.88% in the 4th grade. The Gencha sample was characterized by lower values (by 1–2 seconds) in group I children. The difference in AP in the 1st grade was 4.73 sec., then in the 4th grade –6.86 sec. The Schulte tables showed higher values of work efficiency, workability and endurance coefficient along with a decrease in speed and strength indicators and physical endurance.Conclusions: by the end of primary school, the vegetative balance in group I children is between normo- and sympathicotonia. The Gencha test made it possible to establish insufficient resistance of the body to hypoxia. Children of group II demonstrated better adaptive capabilities of the CCC, speed and strength indicators and physical endurance. The data obtained must be taken into account in the pedagogical process with younger schoolchildren and require the development of differentiated recreational activities.
目的：研究不同学业成绩的小学生在学习动态中的身体机能状态和工作能力指标。第一组--82 名学习成绩优异的学生 第二组--491 名学习成绩良好的儿童。采用 F. Halberg 的方法测定了柯尔多植物性神经功能指数（VIC）、鲁菲尔测试、玄奇测试和适应指数（AP）。结果显示：第一组儿童的VIC值是第二组的2.5-3.5倍。各组之间的 IR 差异增大，四年级时达到 32.88%。Gencha 样本的特点是 I 组儿童的数值较低（1-2 秒）。一年级的 AP 差值为 4.73 秒，四年级为-6.86 秒。舒尔特表显示，工作效率、工作能力和耐力系数的数值较高，而速度和力量指标以及身体耐力则有所下降。根查试验可以确定身体对缺氧的抵抗力不足。第二组儿童的 CCC 适应能力、速度和力量指标以及体能耐力都较好。在对年龄较小的学龄儿童进行教学的过程中，必须考虑到所获得的数据，并需要开展有区别的娱乐活动。
{"title":"Dynamics of indicators of the functional state of the body and working capacity in young schoolchildren with different school success","authors":"A. M. Levchin, A. Lebedenko, I. B. Ershova, A. G. Rogovtsova","doi":"10.21886/2219-8075-2023-14-4-114-121","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-114-121","url":null,"abstract":"Objective: to study the indicators of the functional state of the body and working capacity in the dynamics of learning in elementary school children with different school success.Materials and methods: the study included 573 elementary school students. Group I – 82 students with excellent academic performance Group II – 491 children with good academic performance. The vegetative index of Kerdo (VIC), the Rufier test, the Genchi test, and the adaptive index (AP) were determined by the method of F. Halberg. The mental performance of younger schoolchildren was studied using Schulte tables.Results: the values of VIC in group I children were 2.5–3.5 times higher. The difference in IR between the groups increased, amounting to 32.88% in the 4th grade. The Gencha sample was characterized by lower values (by 1–2 seconds) in group I children. The difference in AP in the 1st grade was 4.73 sec., then in the 4th grade –6.86 sec. The Schulte tables showed higher values of work efficiency, workability and endurance coefficient along with a decrease in speed and strength indicators and physical endurance.Conclusions: by the end of primary school, the vegetative balance in group I children is between normo- and sympathicotonia. The Gencha test made it possible to establish insufficient resistance of the body to hypoxia. Children of group II demonstrated better adaptive capabilities of the CCC, speed and strength indicators and physical endurance. The data obtained must be taken into account in the pedagogical process with younger schoolchildren and require the development of differentiated recreational activities.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-28DOI: 10.21886/2219-8075-2023-14-4-58-65
E. Khalturina, I. V. Nesterova, V. V. Malinovskaya, M. S. Myandiev
Objective: to study the characteristics of the functioning of the IFN system, the presence of autoantibodies to INFα in patients suffering from atypical chronic active herpesvirus infections (ACA-HVI) in comparison with patients with a typical course of chronic herpesvirus infections (CHVI).Materials and methods: under our supervision were 485 patients of both sexes aged 23 to 70 years, suffering from chronic herpes virus infections, of which 335 patients suffered from AHA-HVI and 150 people suffered from CHVI. The comparison group was 250 conditionally healthy individuals (CG). The complex of the study included methods for detecting herpesviruses: serodiagnostics, PCR-RT. The IFN system (spontaneous and induced production, serum concentration) was tested by ELISA. The study was approved by the ethics board and informed consent was obtained from all patients.Results: the incidence of various mono-mixed herpesvirus infections in patients with ACA-HVI (mono – 26,6% and mixed – 73.4%) and CHVI (mono – 23.1% and mixed – 76.9%) was determined, with EBV dominance in patients of both groups. Serum IFNα deficiency was detected in 100% of cases in both groups, and IFNα in 67% in ACA-HVI and 57% in CHVI. At the same time, significant differences were found between the ACA-HVI and HGVI groups in the level of IFNα reduction: 10 and 5 times, respectively, and for IFNγ – 2.0 and 2.6 times, respectively. The induced IFNα production decreased by 89.1% in ACA-HVI and 47.2% in CHVI. A decrease in induced IFNγ production is characteristic of 50% of patients in both groups. At the same time, the level of induced production of IFN α in patients with ACA-HVI was 9 times lower than in the control group and 4.75 times lower than in the group of patients with CHVI. And the level of induced IFNγ production was 2 times lower compared to CHVI and the control group.Conclusions: when assessing the state of the IFN system in patients with various chronic herpes virus infections, significant differences were revealed. Thus, the most pronounced manifestations of interferonopathy, consisting in a significant decrease in serum IFNα and IFNγ and defects in induced IFN production of both types, are observed statistically significantly more often in the group of patients with an atypical course of the disease than in the group of patients with a typical course of CHVI. The most pronounced disorders in the IFN system and the lack of recovery of IFNα and IFNγ levels in the interrelational period cause atypicity of the course and active viral replication in patients with ACA-HVI.
{"title":"Interferon system in typical chronic and atypically occurring chronic active herpes virus infections","authors":"E. Khalturina, I. V. Nesterova, V. V. Malinovskaya, M. S. Myandiev","doi":"10.21886/2219-8075-2023-14-4-58-65","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-58-65","url":null,"abstract":"Objective: to study the characteristics of the functioning of the IFN system, the presence of autoantibodies to INFα in patients suffering from atypical chronic active herpesvirus infections (ACA-HVI) in comparison with patients with a typical course of chronic herpesvirus infections (CHVI).Materials and methods: under our supervision were 485 patients of both sexes aged 23 to 70 years, suffering from chronic herpes virus infections, of which 335 patients suffered from AHA-HVI and 150 people suffered from CHVI. The comparison group was 250 conditionally healthy individuals (CG). The complex of the study included methods for detecting herpesviruses: serodiagnostics, PCR-RT. The IFN system (spontaneous and induced production, serum concentration) was tested by ELISA. The study was approved by the ethics board and informed consent was obtained from all patients.Results: the incidence of various mono-mixed herpesvirus infections in patients with ACA-HVI (mono – 26,6% and mixed – 73.4%) and CHVI (mono – 23.1% and mixed – 76.9%) was determined, with EBV dominance in patients of both groups. Serum IFNα deficiency was detected in 100% of cases in both groups, and IFNα in 67% in ACA-HVI and 57% in CHVI. At the same time, significant differences were found between the ACA-HVI and HGVI groups in the level of IFNα reduction: 10 and 5 times, respectively, and for IFNγ – 2.0 and 2.6 times, respectively. The induced IFNα production decreased by 89.1% in ACA-HVI and 47.2% in CHVI. A decrease in induced IFNγ production is characteristic of 50% of patients in both groups. At the same time, the level of induced production of IFN α in patients with ACA-HVI was 9 times lower than in the control group and 4.75 times lower than in the group of patients with CHVI. And the level of induced IFNγ production was 2 times lower compared to CHVI and the control group.Conclusions: when assessing the state of the IFN system in patients with various chronic herpes virus infections, significant differences were revealed. Thus, the most pronounced manifestations of interferonopathy, consisting in a significant decrease in serum IFNα and IFNγ and defects in induced IFN production of both types, are observed statistically significantly more often in the group of patients with an atypical course of the disease than in the group of patients with a typical course of CHVI. The most pronounced disorders in the IFN system and the lack of recovery of IFNα and IFNγ levels in the interrelational period cause atypicity of the course and active viral replication in patients with ACA-HVI.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139219886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-26DOI: 10.21886/2219-8075-2023-14-4-35-43
E. Frolov, F. I. Abdulaeva, U. A. Gornostaeva, T. Latysheva, E. Latysheva, G. E. Aminova
GATA2 deficiency is a rare disease belonging to the group of phagocyte birth defects, which is clinically manifested by four syndromes: MonoMac syndrome (myedysplasia and immunodeficiency associated with the development of infections caused by Mycobacterium avium complex); monocyte, dendritic cell, B- and NK-lymphocyte deficiency syndrome; Emberger syndrome, including primary lymphedema with myelodysplasia and sensorineural hearing loss, as well as familial myelodysplastic syndrome and acute myeloid leukemia. The disease is inherited by autosomal dominant type, but in most cases, mutations ofthe germ line of the GATA2 gene occur de novo. The first manifestations of the disease occur in early adulthood, the course of GATA2 deficiency is variable and may differ in individuals in the same family with similar genetic variants. The article presents a clinical case of manifestation of GATA2 deficiency at the age of seven years in the form of development of generalized verrucosis, lymphostasis of the lower limb, generalized tuberculosis with involvement of the abdominal cavity, small pelvis, and chest organs. The examination revealed deficiency of monocytes, B- and NK-lymphocytes, myelodysplastic syndrome with multilineage dysplasia. We present a detailed description of the clinical picture and peculiarities of the course of the primary immunodeficiency state, the results of the examination and treatment.
{"title":"Features of the clinical picture and course of GATA2 deficiency complicated by generalized verrucosis with an outcome in myelodysplastic syndrome in adulthood","authors":"E. Frolov, F. I. Abdulaeva, U. A. Gornostaeva, T. Latysheva, E. Latysheva, G. E. Aminova","doi":"10.21886/2219-8075-2023-14-4-35-43","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-35-43","url":null,"abstract":"GATA2 deficiency is a rare disease belonging to the group of phagocyte birth defects, which is clinically manifested by four syndromes: MonoMac syndrome (myedysplasia and immunodeficiency associated with the development of infections caused by Mycobacterium avium complex); monocyte, dendritic cell, B- and NK-lymphocyte deficiency syndrome; Emberger syndrome, including primary lymphedema with myelodysplasia and sensorineural hearing loss, as well as familial myelodysplastic syndrome and acute myeloid leukemia. The disease is inherited by autosomal dominant type, but in most cases, mutations ofthe germ line of the GATA2 gene occur de novo. The first manifestations of the disease occur in early adulthood, the course of GATA2 deficiency is variable and may differ in individuals in the same family with similar genetic variants. The article presents a clinical case of manifestation of GATA2 deficiency at the age of seven years in the form of development of generalized verrucosis, lymphostasis of the lower limb, generalized tuberculosis with involvement of the abdominal cavity, small pelvis, and chest organs. The examination revealed deficiency of monocytes, B- and NK-lymphocytes, myelodysplastic syndrome with multilineage dysplasia. We present a detailed description of the clinical picture and peculiarities of the course of the primary immunodeficiency state, the results of the examination and treatment.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139235944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.21886/2219-8075-2023-14-4-22-28
N. Skripkina, D. Sizyakin, E. A. Antonova
Objective: to study the clinical and immunological parameters of patients with COVID-19 in moderate form with varying degrees of lung tissue damage.Materials and methods: 80 patients hospitalized with a diagnosis of «COVID-19, moderate form; complication: bilateral polysegmental interstitial pneumonia» were examined. The difference in laboratory parameters (general clinical, biochemical and immunological) was evaluated depending on the degree of lung damage (up to 25 and up to 50%). Comparison group: 20 healthy volunteers.Results: the differences between patients with different lung lesion areas consisted in higher levels of prognosis severity markers – CRP, LDH. In the immune status in the CT group 2, there was a decrease in CD3+ lymphocytes and their helper subpopulation, dysimmunoglobulinemia and a higher IL-6 content.Conclusions: CT examination is of great importance for monitoring the progression of the disease. The smaller area of lung lesion reflects a lighter course of the disease, which does not exclude the need for dispensary observation and rehabilitation after discharge.
{"title":"Clinical and immunological characteristics of patients with moderate COVID-19 with different lung lesion areas","authors":"N. Skripkina, D. Sizyakin, E. A. Antonova","doi":"10.21886/2219-8075-2023-14-4-22-28","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-22-28","url":null,"abstract":"Objective: to study the clinical and immunological parameters of patients with COVID-19 in moderate form with varying degrees of lung tissue damage.Materials and methods: 80 patients hospitalized with a diagnosis of «COVID-19, moderate form; complication: bilateral polysegmental interstitial pneumonia» were examined. The difference in laboratory parameters (general clinical, biochemical and immunological) was evaluated depending on the degree of lung damage (up to 25 and up to 50%). Comparison group: 20 healthy volunteers.Results: the differences between patients with different lung lesion areas consisted in higher levels of prognosis severity markers – CRP, LDH. In the immune status in the CT group 2, there was a decrease in CD3+ lymphocytes and their helper subpopulation, dysimmunoglobulinemia and a higher IL-6 content.Conclusions: CT examination is of great importance for monitoring the progression of the disease. The smaller area of lung lesion reflects a lighter course of the disease, which does not exclude the need for dispensary observation and rehabilitation after discharge.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"5 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139256274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-20DOI: 10.21886/2219-8075-2023-14-4-17-21
L. Sizyakina, I. I. Andreeva, M. Kharitonova
Objective: comparative characteristics of the subpopulation composition of B- lymphocytes and the cytokine spectrum of peripheral blood in infectious and non-infectious manifestations of CVID.Materials and methods: the results of observation of 10 people diagnosed with CVID have been presented. Six patients the clinical manifestation had an infectious phenotype of the disease of synopulmonary localization. Four patients were diagnosed with Crohn's disease, hemolytic anemia, and autoimmune hepatitis. The level of cytokines IL-4, IL-10, IL-17, TNF-α, IFN-γ in blood serum was determined by the ELISA, the phenotypic characteristic of B cells of peripheral blood cells was carried out by flow cytofluorimetry.Results: the proportion of memory B cells in the healthy donors is 30% of all B-lymphocytes, on infectious manifestation of CVID – 12%, with autoimmune – 14%. The number of switched memory B-lymphocytes relative to the total pool of B-2 cells in infectious manifestation is greater (2.3%) than in autoimmune ones (1.4%). In patients with infectious manifestations the content of IFN-γ and TNF-α was increased inrelation to healthy donors of the comparison group, there were no differences in the serum amount of IL-17, with autoimmune manifestations, the content of all these cytokines was increased, IFN-γ and TNF-α being to a greater extent.Conclusion: impaired formation of memory B-lymphocytes and cytokine dysregulation of immune processes are detected regardless of the variant of clinical manifestation of CVID. The nature and degree of changes differ depending on the clinical phenotype of the disease.
{"title":"B-2 lymphocytes and the balance of pro and anti-inflammatory cytokines in infectious and autoimmune phenotypes of common variable immune deficiency","authors":"L. Sizyakina, I. I. Andreeva, M. Kharitonova","doi":"10.21886/2219-8075-2023-14-4-17-21","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-17-21","url":null,"abstract":"Objective: comparative characteristics of the subpopulation composition of B- lymphocytes and the cytokine spectrum of peripheral blood in infectious and non-infectious manifestations of CVID.Materials and methods: the results of observation of 10 people diagnosed with CVID have been presented. Six patients the clinical manifestation had an infectious phenotype of the disease of synopulmonary localization. Four patients were diagnosed with Crohn's disease, hemolytic anemia, and autoimmune hepatitis. The level of cytokines IL-4, IL-10, IL-17, TNF-α, IFN-γ in blood serum was determined by the ELISA, the phenotypic characteristic of B cells of peripheral blood cells was carried out by flow cytofluorimetry.Results: the proportion of memory B cells in the healthy donors is 30% of all B-lymphocytes, on infectious manifestation of CVID – 12%, with autoimmune – 14%. The number of switched memory B-lymphocytes relative to the total pool of B-2 cells in infectious manifestation is greater (2.3%) than in autoimmune ones (1.4%). In patients with infectious manifestations the content of IFN-γ and TNF-α was increased inrelation to healthy donors of the comparison group, there were no differences in the serum amount of IL-17, with autoimmune manifestations, the content of all these cytokines was increased, IFN-γ and TNF-α being to a greater extent.Conclusion: impaired formation of memory B-lymphocytes and cytokine dysregulation of immune processes are detected regardless of the variant of clinical manifestation of CVID. The nature and degree of changes differ depending on the clinical phenotype of the disease.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"29 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-18DOI: 10.21886/2219-8075-2023-14-4-5-10
M. Levkovich, N. V. Ermolova, I. Krukier, V. Avrutskaya, Yu.A. Petrov, A. A. Grigoryants, A. V. Zabayrachnaya
Objective: to establish the features of cytokine production in patients of reproductive age with external genital endometriosis at various stages of the disease.Materials and methods: the study included 52 patients with external genital endometriosis (EGE). Group I consisted of 31 patients with stages I–II of EGE, group II — 21 patients with stages III–IV of the disease, control group — 20 women without endometriosis. Serum samples were obtained before anesthesia, and peritoneal fluid (PF) samples were obtained during endoscopic examination of patients. Determination of the content of cytokines in blood serum and peritoneal fluid was carried out by enzyme immunoassay using a multifunctional counter for enzyme immunoassays with Victor software (Finland). TNF-α, IL-10 — using reagents from BenderMed Systems (Austria). IL-13, IL-18 using reagents from Cloud-CloneCorp (USA) according to the manufacturer’s recommendations. Application software packages (Megastat and Statistica 10.0) were used to create a database and conduct statistical research. To determine statistical differences between groups, the Mann-Whitney test was used for independent groups and the Wilcoxon test for dependent groups. P values less than 0.05 were considered statistically significant. Data are presented as median and quartiles.Results: at stages I–II of EGE, an increase in the level of TNF-α, IL-18 was detected in the blood serum and peritoneal fluid, which can stimulate proliferation, invasion of endometrial cells and neovascularization; stage III–IV of the disease was characterized by an increase in the level of TNF-α, IL-10, IL-13 in the peritoneal fluid, which promotes disease progression by evading ectopic endometrial cells from immune control.Conclusion: the identified features of the production of pro- and anti-inflammatory cytokines at the systemic and local level at various stages of EGE confirm their importance in the development of the disease. Further research is needed to understand the mechanisms underlying the progression of NGE and to develop methods for early diagnosis.
材料与方法：研究包括 52 名外生殖器子宫内膜异位症（EGE）患者。第一组包括 31 名 I-II 期 EGE 患者，第二组--21 名 III-IV 期患者，对照组--20 名未患子宫内膜异位症的妇女。血清样本在麻醉前采集，腹腔液（PF）样本在对患者进行内窥镜检查时采集。血清和腹腔液中细胞因子含量的测定是通过酶免疫测定法进行的，使用的是带有维克多软件（芬兰）的酶免疫测定多功能计数器。TNF-α、IL-10--使用 BenderMed Systems 公司（奥地利）的试剂。IL-13、IL-18 根据制造商的建议使用 Cloud-CloneCorp（美国）公司的试剂。应用软件包（Megastat 和 Statistica 10.0）用于创建数据库和进行统计研究。为确定组间统计差异，对独立组采用曼-惠特尼检验，对因果组采用威尔科克逊检验。P 值小于 0.05 即为具有统计学意义。结果：在 EGE 的 I-II 期，血清和腹腔液中检测到 TNF-α、IL-18 水平升高，可刺激子宫内膜细胞增殖、侵袭和新生血管形成；疾病的 III-IV 期以腹腔液中 TNF-α、IL-10、IL-13 水平升高为特征，通过使异位子宫内膜细胞逃避免疫控制而促进疾病进展。结论：在 EGE 的不同阶段，全身和局部水平的促炎和抗炎细胞因子的产生特点证实了它们在疾病发展中的重要性。要了解 NGE 的发展机制并开发早期诊断方法，还需要进一步的研究。
{"title":"Features of cytokine production at the systemic and local level in patients of reproductive age with external genital endometriosis","authors":"M. Levkovich, N. V. Ermolova, I. Krukier, V. Avrutskaya, Yu.A. Petrov, A. A. Grigoryants, A. V. Zabayrachnaya","doi":"10.21886/2219-8075-2023-14-4-5-10","DOIUrl":"https://doi.org/10.21886/2219-8075-2023-14-4-5-10","url":null,"abstract":"Objective: to establish the features of cytokine production in patients of reproductive age with external genital endometriosis at various stages of the disease.Materials and methods: the study included 52 patients with external genital endometriosis (EGE). Group I consisted of 31 patients with stages I–II of EGE, group II — 21 patients with stages III–IV of the disease, control group — 20 women without endometriosis. Serum samples were obtained before anesthesia, and peritoneal fluid (PF) samples were obtained during endoscopic examination of patients. Determination of the content of cytokines in blood serum and peritoneal fluid was carried out by enzyme immunoassay using a multifunctional counter for enzyme immunoassays with Victor software (Finland). TNF-α, IL-10 — using reagents from BenderMed Systems (Austria). IL-13, IL-18 using reagents from Cloud-CloneCorp (USA) according to the manufacturer’s recommendations. Application software packages (Megastat and Statistica 10.0) were used to create a database and conduct statistical research. To determine statistical differences between groups, the Mann-Whitney test was used for independent groups and the Wilcoxon test for dependent groups. P values less than 0.05 were considered statistically significant. Data are presented as median and quartiles.Results: at stages I–II of EGE, an increase in the level of TNF-α, IL-18 was detected in the blood serum and peritoneal fluid, which can stimulate proliferation, invasion of endometrial cells and neovascularization; stage III–IV of the disease was characterized by an increase in the level of TNF-α, IL-10, IL-13 in the peritoneal fluid, which promotes disease progression by evading ectopic endometrial cells from immune control.Conclusion: the identified features of the production of pro- and anti-inflammatory cytokines at the systemic and local level at various stages of EGE confirm their importance in the development of the disease. Further research is needed to understand the mechanisms underlying the progression of NGE and to develop methods for early diagnosis.","PeriodicalId":18314,"journal":{"name":"Medical Herald of the South of Russia","volume":"61 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139262429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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