The Effects of Conventional Semen, Sexed-Semen and Embryo Transfer on Pregnancy Rate in Dairy Cows

Davut Koca, A. Aktar, Ali Osman Turgut, H. Sagirkaya, S. Alçay
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Abstract

In this study, it was aimed to detect the effects of conventional semen, sexed-semen and embryo transfer on pregnancy rates in dairy Holstein cows. In the study, a total of 139 healthy cows with the serum progesterone > 8 ng/ml were used as animal material. Estrus synchronization protocol was applied and cows were divided into three different groups as conventional semen group (G1, n=46), sexed semen group (G2, n=47), and embryo transfer group (G3, n=46) considering age, body condition score, lactation number, and live weight. Cows in G1 and G2 were inseminated with conventional semen and sexed-semen respectively. Embryo transfer was performed to cows in Group 3 (n=46) 7th day after estrus. SPSS was used for statistical evaluation of results (SPSS 23, Chicago, IL, USA). Pregnancies were detected 30th and 60th day after artificial insemination and embryo transfer. Pregnancy rates were detected as %50, %46,8 and %69,56 in G1, G2, and G3 respectively on 30th day. On 60th day, however, pregnancy rates were %45.60, %42.55 and %67.39 in G1, G2, and G3 respectively due to embryonic losses. The pregnancy rate was significantly higher in the embryo transfer group (G3) compared to the conventional semen (G1) and sexed-semen (G2) group (p0.05). Findings led to the conclusion that higher serum progesterone level may increase pregnancy rate in cattle. In addition, embryo transfer may be more advantageous than conventional and sexed-semen to increase genetic progress in dairy cattle.
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常规精液、有性精液和胚胎移植对奶牛妊娠率的影响
本研究旨在检测常规精液、性别化精液和胚胎移植对荷斯坦奶牛怀孕率的影响。研究共使用了 139 头血清孕酮大于 8 纳克/毫升的健康奶牛作为动物材料。考虑到年龄、体况评分、泌乳数和活重,将奶牛分为三个不同的组,即传统精液组(G1,46 头)、性别精液组(G2,47 头)和胚胎移植组(G3,46 头)。G1和G2组的奶牛分别使用常规精液和性别精液进行人工授精。发情后第 7 天,对第 3 组奶牛(n=46)进行胚胎移植。统计结果采用 SPSS(SPSS 23,芝加哥,伊利诺斯州,美国)。人工授精和胚胎移植后第 30 天和第 60 天检测妊娠情况。第 30 天,G1、G2 和 G3 的妊娠率分别为 50%、46.8% 和 69.56%。但在第 60 天,由于胚胎损失,G1、G2 和 G3 的妊娠率分别为 45.60%、42.55% 和 67.39%。胚胎移植组(G3)的妊娠率明显高于传统精液组(G1)和性别化精液组(G2)(P0.05)。研究结果得出结论,较高的血清孕酮水平可提高牛的妊娠率。此外,胚胎移植可能比传统精液和性别化精液更有利于提高奶牛的遗传进展。
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