The KISS1 gene, which activates G protein-coupled receptor-54 (GPR54) and plays a crucial role in the neuroendocrine regulation of GnRH secretion, is known to encode a family of neuropeptides called kisspeptins. Therefore, the KISS1 gene is thought to be associated with sexual maturity, offspring development, and estrus. The g.2124T>A polymorphism of the KISS1 gene, is believed to affect the reproductive system, has been studied in Indonesian goat breeds, Damascus, Zarabi, Baladi, Gaddi, Kaligesing, Guanzong, Xianong Saanen and Boer goat breeds until now. These studies are generally related to determining polymorphism frequencies or the effect of genotype on litter size. No studies have been found on the effect of the KISS1 gene on luteal structures, especially in Saanen goats. The study aims to determine the allele and genotype frequencies of the KISS1 gene g.2124T>A SNP by the PCR-RFLP method and define the relationship between the genotypic structures and reproductive parameters such as luteal growth, maximum corpus luteum diameter, and mating rate in Saanen goats (n=30). According to the results, the genotype frequencies in Saanen goats for the KISS1 gene g.2124T>A were 10.35%, 55.17%, and 34.48% for AA, TT and AT, respectively. Moreover, the genotypic structure did not have a statistically significant effect on investigated fertility characteristics. The average values of expected heterozygosity (He), observed heterozygosity (Ho), the effective number of alleles (Ne), fixation index values (FIS), and the polymorphism information content (PIC) were 0.3996, 0.3448, 1.6655, 0.8629 and 0.855, respectively. The Hardy-Weinberg chi-square (χ2) value was found to be 0.5435. In conclusion, it was found necessary to study the KISS1 - g.2124T>A polymorphism in larger herds with different gene pools as a reason for the existence of genotypic variation and the narrow population size.
{"title":"Detection of the effect of the KISS1 gene on reproductive parameters in Saanen goats","authors":"D. Dinçel, Mehmed Berk Toker, I. Dogan","doi":"10.30782/jrvm.1449054","DOIUrl":"https://doi.org/10.30782/jrvm.1449054","url":null,"abstract":"The KISS1 gene, which activates G protein-coupled receptor-54 (GPR54) and plays a crucial role in the neuroendocrine regulation of GnRH secretion, is known to encode a family of neuropeptides called kisspeptins. Therefore, the KISS1 gene is thought to be associated with sexual maturity, offspring development, and estrus. The g.2124T>A polymorphism of the KISS1 gene, is believed to affect the reproductive system, has been studied in Indonesian goat breeds, Damascus, Zarabi, Baladi, Gaddi, Kaligesing, Guanzong, Xianong Saanen and Boer goat breeds until now. These studies are generally related to determining polymorphism frequencies or the effect of genotype on litter size. No studies have been found on the effect of the KISS1 gene on luteal structures, especially in Saanen goats. The study aims to determine the allele and genotype frequencies of the KISS1 gene g.2124T>A SNP by the PCR-RFLP method and define the relationship between the genotypic structures and reproductive parameters such as luteal growth, maximum corpus luteum diameter, and mating rate in Saanen goats (n=30). According to the results, the genotype frequencies in Saanen goats for the KISS1 gene g.2124T>A were 10.35%, 55.17%, and 34.48% for AA, TT and AT, respectively. Moreover, the genotypic structure did not have a statistically significant effect on investigated fertility characteristics. The average values of expected heterozygosity (He), observed heterozygosity (Ho), the effective number of alleles (Ne), fixation index values (FIS), and the polymorphism information content (PIC) were 0.3996, 0.3448, 1.6655, 0.8629 and 0.855, respectively. The Hardy-Weinberg chi-square (χ2) value was found to be 0.5435. In conclusion, it was found necessary to study the KISS1 - g.2124T>A polymorphism in larger herds with different gene pools as a reason for the existence of genotypic variation and the narrow population size.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"26 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141836008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryptosporidium parvum is a zoonotic protozoan that causes neonatal calf diarrhea common in the world. Buparvaquone, known to have very positive effects on oocysts, which are the central reserve in the spread of infection, has not yet been investigated for its hemato-biochemical aspect in calves with cryptosporidiosis. The present study investigated the effect of buparvaquone on renal and hepatic functions in naturally infected newborn calves with cryptosporidiosis. A group was formed for this study using only a total of ten calves (n=10) naturally infected with cryptosporidiosis. Buparvaquone 2.5 mg/kg intramuscular injection was administered as a single dose to all calves in the group. Laboratory analyses and statistical calculations of blood and serum samples taken on the specified days were made. According to the results we obtained in the study, it was observed that the renal and hepatic effects of the drug after a single dose injection of buparvaquone to calves with neonatal cryptosporidiosis remained within normal limits, similar to the methods safely applied in the treatment of cryptosporidiosis in the field.
{"title":"Evaluation of the effect of buparvaquone used in the treatment of neonatal calves naturally infected with cryptosporidiosis on renal and hepatic functions","authors":"Fatma Zehra EVCİ GÜNEY, Sezgin Şentürk","doi":"10.30782/jrvm.1358532","DOIUrl":"https://doi.org/10.30782/jrvm.1358532","url":null,"abstract":"Cryptosporidium parvum is a zoonotic protozoan that causes neonatal calf diarrhea common in the world. Buparvaquone, known to have very positive effects on oocysts, which are the central reserve in the spread of infection, has not yet been investigated for its hemato-biochemical aspect in calves with cryptosporidiosis. The present study investigated the effect of buparvaquone on renal and hepatic functions in naturally infected newborn calves with cryptosporidiosis. A group was formed for this study using only a total of ten calves (n=10) naturally infected with cryptosporidiosis. Buparvaquone 2.5 mg/kg intramuscular injection was administered as a single dose to all calves in the group. Laboratory analyses and statistical calculations of blood and serum samples taken on the specified days were made. According to the results we obtained in the study, it was observed that the renal and hepatic effects of the drug after a single dose injection of buparvaquone to calves with neonatal cryptosporidiosis remained within normal limits, similar to the methods safely applied in the treatment of cryptosporidiosis in the field.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"115 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139134560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışma bir gıda sanayi artığı olan ay çekirdeği kabuğunun piliç eti üretiminde altlık materyali olarak kullanımını araştırmak amacıyla yapılmıştır. Çalışmada ay çekirdeği kabuğu ile piliç eti üretiminde yaygın olarak kullanılan büyük parçacıklı planya talaşı, ince toz talaş, çeltik kavuzu ve balya samanının büyüme performansı, altlık kalitesi ve hayvan refahı üzerine olan etkileri karşılaştırılmıştır. Her grupta 1000 adet günlük yaşta civciv olmak üzere denemede 5000 adet, günlük yaşta etlik civciv kullanılmıştır. Bütün gruplarda yer alan hayvanlar altlık hariç, etlik civciv/piliçler için eşdeğer standart koşullarda bakılmışlardır. Denemede yer alan hayvanlar deneme süresince ticari etlik civciv/piliç yemleri ile beslenmişlerdir. Farklı altlık materyallerinin dönem sonu canlı ağırlık ve ölüm oranı üzerine etkisi önemsiz bulunmuştur (P>0.05). Çalışma genelinde ayak tabanında değişik şiddette lezyon ve leke oluşumu gözlenen hayvan sayısı diz ekleminde lezyon şekillenen hayvan sayısına göre daha fazla bulunmuş, bütün gruplarda göğüs ve karın tüyleri kirlilik düzeyi en şiddetli (skor 7 ve 8) düzeyde olan hayvana rastlanmamıştır. Altlık pH ve rutubet değerleri ay çekirdeği kabuğu grubunda en düşük bulunurken, balya samanı altlık grubunda en yüksek bulunmuştur (P<0.001). Çalışmadan elde edilen veriler bütünüyle değerlendirildiğinde; ay çekirdeği kabuğunun büyüme performansını olumsuz etkilemeden piliç eti üretiminde altlık olarak kullanılabileceği sonucuna ulaşılmıştır.
{"title":"Ay Çekirdeği Kabuğunun Piliç Eti Üretiminde Altlık Materyali Olarak Kullanımı Üzerine Bir Çalışma: Etlik Piliç Büyüme Performansı, Altlık Kalitesi ve Hayvan Refahı Üzerine Etkisi","authors":"M. Petek, İbrahim Eren Lapaci","doi":"10.30782/jrvm.1374018","DOIUrl":"https://doi.org/10.30782/jrvm.1374018","url":null,"abstract":"Bu çalışma bir gıda sanayi artığı olan ay çekirdeği kabuğunun piliç eti üretiminde altlık materyali olarak kullanımını araştırmak amacıyla yapılmıştır. Çalışmada ay çekirdeği kabuğu ile piliç eti üretiminde yaygın olarak kullanılan büyük parçacıklı planya talaşı, ince toz talaş, çeltik kavuzu ve balya samanının büyüme performansı, altlık kalitesi ve hayvan refahı üzerine olan etkileri karşılaştırılmıştır. Her grupta 1000 adet günlük yaşta civciv olmak üzere denemede 5000 adet, günlük yaşta etlik civciv kullanılmıştır. Bütün gruplarda yer alan hayvanlar altlık hariç, etlik civciv/piliçler için eşdeğer standart koşullarda bakılmışlardır. Denemede yer alan hayvanlar deneme süresince ticari etlik civciv/piliç yemleri ile beslenmişlerdir. Farklı altlık materyallerinin dönem sonu canlı ağırlık ve ölüm oranı üzerine etkisi önemsiz bulunmuştur (P>0.05). Çalışma genelinde ayak tabanında değişik şiddette lezyon ve leke oluşumu gözlenen hayvan sayısı diz ekleminde lezyon şekillenen hayvan sayısına göre daha fazla bulunmuş, bütün gruplarda göğüs ve karın tüyleri kirlilik düzeyi en şiddetli (skor 7 ve 8) düzeyde olan hayvana rastlanmamıştır. Altlık pH ve rutubet değerleri ay çekirdeği kabuğu grubunda en düşük bulunurken, balya samanı altlık grubunda en yüksek bulunmuştur (P<0.001). Çalışmadan elde edilen veriler bütünüyle değerlendirildiğinde; ay çekirdeği kabuğunun büyüme performansını olumsuz etkilemeden piliç eti üretiminde altlık olarak kullanılabileceği sonucuna ulaşılmıştır.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"50 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139180941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study is to examine the textural structure and organic functional groups of horse-donkey, cow, dog, and sheep bones by nitrogen gas adsorption-desorption and Fourier Transform Infrared (FT-IR) spectroscopic methods, respectively. For this purpose, textural properties such as BET (Brunauer-Emmett-Teller) surface area, total pore volume, average pore diameter, and surface organic functional groups were determined by nitrogen gas adsorption/desorption at -196 oC and FT-IR spectral analysis methods, respectively. It was observed that the nitrogen gas adsorption isotherms obtained from the BET analysis results were similar to Type-V, which indicates that they have a mesoporous and/or macroporous textural structure, in the IUPAC nitrogen gas isotherm classification. In addition, it was determined from the FT-IR analysis results that they mainly contain organic functional groups such as amine, alcohol, carboxylic acid, ester, ether.
本研究的目的是通过氮气吸附-解吸法和傅立叶变换红外（FT-IR）光谱法分别研究驴骨、牛骨、狗骨和羊骨的纹理结构和有机官能团。为此，分别在 -196 oC 温度下用氮气吸附/解吸法和傅立叶变换红外光谱分析法测定了 BET（布鲁纳-艾美特-泰勒）表面积、总孔体积、平均孔直径和表面有机官能团等纹理特性。结果表明，根据 BET 分析结果得到的氮气吸附等温线类似于 IUPAC 氮气等温线分类中的 Type-V，这表明它们具有介孔和/或大孔质地结构。此外，根据傅立叶变换红外分析结果确定，它们主要含有有机官能团，如胺、醇、羧酸、酯、醚等。
{"title":"Investigation of textural and surface chemical properties of some animal bones","authors":"Barış Can Güzel","doi":"10.30782/jrvm.1381682","DOIUrl":"https://doi.org/10.30782/jrvm.1381682","url":null,"abstract":"The aim of the study is to examine the textural structure and organic functional groups of horse-donkey, cow, dog, and sheep bones by nitrogen gas adsorption-desorption and Fourier Transform Infrared (FT-IR) spectroscopic methods, respectively. For this purpose, textural properties such as BET (Brunauer-Emmett-Teller) surface area, total pore volume, average pore diameter, and surface organic functional groups were determined by nitrogen gas adsorption/desorption at -196 oC and FT-IR spectral analysis methods, respectively. It was observed that the nitrogen gas adsorption isotherms obtained from the BET analysis results were similar to Type-V, which indicates that they have a mesoporous and/or macroporous textural structure, in the IUPAC nitrogen gas isotherm classification. In addition, it was determined from the FT-IR analysis results that they mainly contain organic functional groups such as amine, alcohol, carboxylic acid, ester, ether.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"24 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu anket çalışması ile Türkiye’de ruminant sağlığı alanında çalışan veteriner hekimlerin mesleki uygulamalarının ve sahada karşılaşılan problemlerin ortaya konması amaçlanmıştır. Mail ile gönderilen ve 34 sorudan oluşan ankete 7 farklı coğrafi bölgedeki 56 ilden toplam 326 veteriner hekim katılmıştır. Çalışmanın sonuçları genel olarak değerlendirildikten sonra veriler; veteriner hekimlerin çalışma şekline göre 3 grup, yaşlarına göre 4 grup ve bölgelerine göre 3 grupta ele alınmıştır. Ankete katılan veteriner hekimlerin mezun olduktan sonra %54,3’ü ilk 6 ay içerisinde kalıcı olarak işe başlamışlardır. Mesleğe yeni başladıklarında en zorlandıkları alanlar şirurjikal operasyonlar (%32,5) ve hayvan besleme (%31,9) olmuştur. Çiftliklerde çalışan hekimlerin kendilerini en zayıf hissettikleri alan olarak hayvan besleme (%60,7) ön plana çıkmıştır. Meslek içi eğitim, kongre vb. etkinliklere %36,3’ün nadir katıldığı, hatta %65,0’inin yeni literatür ve kaynakları takip etmekten uzak olduğu görülmüştür. Veteriner hekimlerin sığırlarda gebelik muayenesinde %54,8’inin ultrasonu sık kullandığı, %24,9’unun ise hiç kullanmadığı gözlenmiştir. Sığırcılıkta en sık karşılaşılan problemler beslenme ve metabolizma hastalıkları (%32,3) ve buzağı hastalıkları (%26,8) olmuştur. Koyun keçi sağlığında ise kuzu-oğlak ölümleri (%51,1), abortlar (%21,7), enfeksiyon hastalıklar (%13,4) ve paraziter hastalıklar (%11,2) öne çıkmıştır. Hekimler bölgelerinde ilk bir aydaki buzağı ölüm oranlarının en çok %6-10 arasında ve kuzu-oğlaklardaki ölüm oranın %10’nun üzerinde olduğunu belirtmişlerdir. Sonuç olarak veteriner fakültelerinin eğitiminde el becerisine dayalı klinik uygulamalara ve ruminantların beslenmesi konularına önem verilmesi gerektiği, mesleki eğitim ve yeni kaynakların takibine ihtiyaç duyulduğu, koruyucu hekimliğe önem verilmesi gerektiği ileri sürülebilir.
{"title":"TÜRKİYE’DE RUMİNANT ALANINDA ÇALIŞAN VETERİNER HEKİMLERİN MESLEKİ UYGULAMALARININ DEĞERLENDİRİLMESİ – I","authors":"Hasan Batmaz, Onur Topal, Hakan Üstüner","doi":"10.30782/jrvm.1310947","DOIUrl":"https://doi.org/10.30782/jrvm.1310947","url":null,"abstract":"Bu anket çalışması ile Türkiye’de ruminant sağlığı alanında çalışan veteriner hekimlerin mesleki uygulamalarının ve sahada karşılaşılan problemlerin ortaya konması amaçlanmıştır. Mail ile gönderilen ve 34 sorudan oluşan ankete 7 farklı coğrafi bölgedeki 56 ilden toplam 326 veteriner hekim katılmıştır. Çalışmanın sonuçları genel olarak değerlendirildikten sonra veriler; veteriner hekimlerin çalışma şekline göre 3 grup, yaşlarına göre 4 grup ve bölgelerine göre 3 grupta ele alınmıştır. Ankete katılan veteriner hekimlerin mezun olduktan sonra %54,3’ü ilk 6 ay içerisinde kalıcı olarak işe başlamışlardır. Mesleğe yeni başladıklarında en zorlandıkları alanlar şirurjikal operasyonlar (%32,5) ve hayvan besleme (%31,9) olmuştur. Çiftliklerde çalışan hekimlerin kendilerini en zayıf hissettikleri alan olarak hayvan besleme (%60,7) ön plana çıkmıştır. Meslek içi eğitim, kongre vb. etkinliklere %36,3’ün nadir katıldığı, hatta %65,0’inin yeni literatür ve kaynakları takip etmekten uzak olduğu görülmüştür. Veteriner hekimlerin sığırlarda gebelik muayenesinde %54,8’inin ultrasonu sık kullandığı, %24,9’unun ise hiç kullanmadığı gözlenmiştir. Sığırcılıkta en sık karşılaşılan problemler beslenme ve metabolizma hastalıkları (%32,3) ve buzağı hastalıkları (%26,8) olmuştur. Koyun keçi sağlığında ise kuzu-oğlak ölümleri (%51,1), abortlar (%21,7), enfeksiyon hastalıklar (%13,4) ve paraziter hastalıklar (%11,2) öne çıkmıştır. Hekimler bölgelerinde ilk bir aydaki buzağı ölüm oranlarının en çok %6-10 arasında ve kuzu-oğlaklardaki ölüm oranın %10’nun üzerinde olduğunu belirtmişlerdir. Sonuç olarak veteriner fakültelerinin eğitiminde el becerisine dayalı klinik uygulamalara ve ruminantların beslenmesi konularına önem verilmesi gerektiği, mesleki eğitim ve yeni kaynakların takibine ihtiyaç duyulduğu, koruyucu hekimliğe önem verilmesi gerektiği ileri sürülebilir.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"223 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139260639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Davut Koca, A. Aktar, Ali Osman Turgut, H. Sagirkaya, S. Alçay
In this study, it was aimed to detect the effects of conventional semen, sexed-semen and embryo transfer on pregnancy rates in dairy Holstein cows. In the study, a total of 139 healthy cows with the serum progesterone > 8 ng/ml were used as animal material. Estrus synchronization protocol was applied and cows were divided into three different groups as conventional semen group (G1, n=46), sexed semen group (G2, n=47), and embryo transfer group (G3, n=46) considering age, body condition score, lactation number, and live weight. Cows in G1 and G2 were inseminated with conventional semen and sexed-semen respectively. Embryo transfer was performed to cows in Group 3 (n=46) 7th day after estrus. SPSS was used for statistical evaluation of results (SPSS 23, Chicago, IL, USA). Pregnancies were detected 30th and 60th day after artificial insemination and embryo transfer. Pregnancy rates were detected as %50, %46,8 and %69,56 in G1, G2, and G3 respectively on 30th day. On 60th day, however, pregnancy rates were %45.60, %42.55 and %67.39 in G1, G2, and G3 respectively due to embryonic losses. The pregnancy rate was significantly higher in the embryo transfer group (G3) compared to the conventional semen (G1) and sexed-semen (G2) group (p0.05). Findings led to the conclusion that higher serum progesterone level may increase pregnancy rate in cattle. In addition, embryo transfer may be more advantageous than conventional and sexed-semen to increase genetic progress in dairy cattle.
{"title":"The Effects of Conventional Semen, Sexed-Semen and Embryo Transfer on Pregnancy Rate in Dairy Cows","authors":"Davut Koca, A. Aktar, Ali Osman Turgut, H. Sagirkaya, S. Alçay","doi":"10.30782/jrvm.1361215","DOIUrl":"https://doi.org/10.30782/jrvm.1361215","url":null,"abstract":"In this study, it was aimed to detect the effects of conventional semen, sexed-semen and embryo transfer on pregnancy rates in dairy Holstein cows. In the study, a total of 139 healthy cows with the serum progesterone > 8 ng/ml were used as animal material. Estrus synchronization protocol was applied and cows were divided into three different groups as conventional semen group (G1, n=46), sexed semen group (G2, n=47), and embryo transfer group (G3, n=46) considering age, body condition score, lactation number, and live weight. Cows in G1 and G2 were inseminated with conventional semen and sexed-semen respectively. Embryo transfer was performed to cows in Group 3 (n=46) 7th day after estrus. SPSS was used for statistical evaluation of results (SPSS 23, Chicago, IL, USA). Pregnancies were detected 30th and 60th day after artificial insemination and embryo transfer. Pregnancy rates were detected as %50, %46,8 and %69,56 in G1, G2, and G3 respectively on 30th day. On 60th day, however, pregnancy rates were %45.60, %42.55 and %67.39 in G1, G2, and G3 respectively due to embryonic losses. The pregnancy rate was significantly higher in the embryo transfer group (G3) compared to the conventional semen (G1) and sexed-semen (G2) group (p0.05). Findings led to the conclusion that higher serum progesterone level may increase pregnancy rate in cattle. In addition, embryo transfer may be more advantageous than conventional and sexed-semen to increase genetic progress in dairy cattle.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139282711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehmet Mustafa Demi̇r, V. Ipek, H. Tutun, Harun Çinar, Leyla Elif Özgü Ayözger
This study aimed to evaluate the wound healing activity of the ointment containing hydroethonolic extract of Salvia tomentosa on a dermal wound model in rabbits. The total phenolic content and DPPH scavenging activity of the hydroethonolic extract of S. tomentosa were determined spectrophotometrically. 2.5% (w/v) and 5% (w/v) ointments were prepared with hydroethanolic extracts of Salvia tomentosa. An in vivo excisional wound model in New Zealand rabbits (n = 35) was used to assess the ointments' wound healing activity. The rabbits were randomly divided into 5 experimental groups, as negative (not receiving any treatment), vehicle (ointment vehicle), positive control (Fitokrem®), 2.5% (w/v) and 5% (w/v) S. tomentasa ointments. The wound diameters were measured using calipers on the 4th, 8th, and 14th days, and histopathological examinations were performed on the 3rd, 7th, and 14th days of treatment. The total phenolic content of S. tomentosa hydroethanolic extract was found to be 61.52 2.33 g of GAE/mg dry plant material. The DPPH scavenging activities of the extract at the concentrations of 10, 100 and 1000 μg/mL were 16.33 ± 3.49, 36.96 ± 7.22 and 72.71 ± 11.50%, respectively. In terms of wound closure, 2.5% and 5% Salvia tomentosa ointments have wound healing activity similar to Fitokrem®. As a conclusion, S. tomentosa has a positive dermal wound healing effect in rabbits.
{"title":"Effects of Salvia tomentosa ointment on dermal wound healing in a rabbit model","authors":"Mehmet Mustafa Demi̇r, V. Ipek, H. Tutun, Harun Çinar, Leyla Elif Özgü Ayözger","doi":"10.30782/jrvm.1217557","DOIUrl":"https://doi.org/10.30782/jrvm.1217557","url":null,"abstract":"This study aimed to evaluate the wound healing activity of the ointment containing hydroethonolic extract of Salvia tomentosa on a dermal wound model in rabbits. The total phenolic content and DPPH scavenging activity of the hydroethonolic extract of S. tomentosa were determined spectrophotometrically. 2.5% (w/v) and 5% (w/v) ointments were prepared with hydroethanolic extracts of Salvia tomentosa. An in vivo excisional wound model in New Zealand rabbits (n = 35) was used to assess the ointments' wound healing activity. The rabbits were randomly divided into 5 experimental groups, as negative (not receiving any treatment), vehicle (ointment vehicle), positive control (Fitokrem®), 2.5% (w/v) and 5% (w/v) S. tomentasa ointments. The wound diameters were measured using calipers on the 4th, 8th, and 14th days, and histopathological examinations were performed on the 3rd, 7th, and 14th days of treatment. The total phenolic content of S. tomentosa hydroethanolic extract was found to be 61.52 2.33 g of GAE/mg dry plant material. The DPPH scavenging activities of the extract at the concentrations of 10, 100 and 1000 μg/mL were 16.33 ± 3.49, 36.96 ± 7.22 and 72.71 ± 11.50%, respectively. In terms of wound closure, 2.5% and 5% Salvia tomentosa ointments have wound healing activity similar to Fitokrem®. As a conclusion, S. tomentosa has a positive dermal wound healing effect in rabbits.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139319752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The motion system consists of two separate parts, the passive motion system and the active motion system. While the passive movement system consists of bones and joints, the active movement system consists of muscles. The strongest connection between bones, joints and muscles is seen in the feet. Feet help different functions in poultry. One of these functions is the movement the perch. Perching is an instinctive behavior, especially in chickens. There are different opinions on the perch. Among these views, the mechanisms reported as TKM and ODFM are mechanisms that support each other. The cartilaginous protrusions of the MFDL and MFHL muscle tendons at the level of the metatarsophalangeal joint and the pits in the tendon sheaths form a zipper-like structure. With the intertwining of these formations, the foot digits are locked around the perch with the weight of the body, thanks to the flexor muscles. In our study, the muscles acting on the hind leg wrist and digit joints of quails and perching pigeons that do not have the ability to perch were examined macroscopically. For this purpose, hind legs of 20 adult quails and 20 adult pigeons were used. Live weights were determined with a precision scale. They were euthanized by cervical dislocation. After the right and left legs of the euthanized animals were separated from the body, their left legs were fixed in 30% formaldehyde solution for dissection. Dissection was carried out simultaneously for both poultries. As a result of the study, it was determined that MFDL and MFHL muscle tendons, cartilage protrusions at the level of the metatarsophalangeal joint and pits in the tendon sheaths were more prominent in pigeons than in quails. If these protrusions and pits perform the perching movement by locking together like a zipper, it can be interpreted that pigeons are more suitable for perching movement than quails anatomically.
{"title":"Macroscopic Investigation of Muscles Affecting The Hind Limb Wrist (Articulatio Tarsi) And Digit Joints (Articulationes Digiti Pedis) in Quail (Coturnix Coturnix) and Pigeon (Columba Livia)","authors":"Fatma Işbilir, İ. Arican","doi":"10.30782/jrvm.1274313","DOIUrl":"https://doi.org/10.30782/jrvm.1274313","url":null,"abstract":"The motion system consists of two separate parts, the passive motion system and the active motion system. While the passive movement system consists of bones and joints, the active movement system consists of muscles. The strongest connection between bones, joints and muscles is seen in the feet. Feet help different functions in poultry. One of these functions is the movement the perch. Perching is an instinctive behavior, especially in chickens. There are different opinions on the perch. Among these views, the mechanisms reported as TKM and ODFM are mechanisms that support each other. The cartilaginous protrusions of the MFDL and MFHL muscle tendons at the level of the metatarsophalangeal joint and the pits in the tendon sheaths form a zipper-like structure. With the intertwining of these formations, the foot digits are locked around the perch with the weight of the body, thanks to the flexor muscles. In our study, the muscles acting on the hind leg wrist and digit joints of quails and perching pigeons that do not have the ability to perch were examined macroscopically. For this purpose, hind legs of 20 adult quails and 20 adult pigeons were used. Live weights were determined with a precision scale. They were euthanized by cervical dislocation. After the right and left legs of the euthanized animals were separated from the body, their left legs were fixed in 30% formaldehyde solution for dissection. Dissection was carried out simultaneously for both poultries. As a result of the study, it was determined that MFDL and MFHL muscle tendons, cartilage protrusions at the level of the metatarsophalangeal joint and pits in the tendon sheaths were more prominent in pigeons than in quails. If these protrusions and pits perform the perching movement by locking together like a zipper, it can be interpreted that pigeons are more suitable for perching movement than quails anatomically.","PeriodicalId":510578,"journal":{"name":"Journal of Research in Veterinary Medicine","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139321202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：